Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,841	A→G	Y512Y (TAT→TAC)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,841	0	A	G	100.0%	195.8 / NA	53	Y512Y (TAT→TAC)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/30);  total (23/30)

GTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCG  >  NC_000913/1245711‑1245976                                                                                                                                   |                                                                                                                                        gTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCa                                                                                                                                <  2:917734/140‑1 (MQ=255)  ttGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCAc                                                                                                                               >  1:711203/1‑140 (MQ=255)          ttGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGccc                                                                                                                       <  1:1024324/140‑1 (MQ=255)          ttGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGccc                                                                                                                       >  2:1024324/1‑140 (MQ=255)          ttGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGccc                                                                                                                       <  1:853290/140‑1 (MQ=255)           tGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGcccc                                                                                                                      <  2:934716/140‑1 (MQ=255)            gCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGccccc                                                                                                                     <  2:868561/140‑1 (MQ=255)             cTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGcccccc                                                                                                                    <  2:740641/140‑1 (MQ=255)              tGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCg                                                                                                                   <  2:672760/140‑1 (MQ=255)                aCTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGtt                                                                                                                 >  1:793508/1‑140 (MQ=255)                 cTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTc                                                                                                                <  1:420293/140‑1 (MQ=255)                  ttAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTcc                                                                                                               >  1:745039/1‑140 (MQ=255)                    aaCGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCgg                                                                                                             <  1:1123427/140‑1 (MQ=255)                     aCGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCggt                                                                                                            <  2:531121/140‑1 (MQ=255)                          cGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCgg                                                                                                             <  1:176085/134‑1 (MQ=255)                          cGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCgg                                                                                                             >  2:176085/1‑134 (MQ=255)                          cGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGc                                                                                                       <  1:352464/140‑1 (MQ=255)                          cGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGc                                                                                                       <  1:722277/140‑1 (MQ=255)                           ggACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCt                                                                                                      <  2:698946/140‑1 (MQ=255)                            gACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGccccc                                                                                                                     <  1:406496/124‑1 (MQ=255)                            gACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGccccc                                                                                                                     >  2:406496/1‑124 (MQ=255)                             aCGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGa                                                                                                    <  2:641505/140‑1 (MQ=255)                                   cGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCgg                                                                                                             <  1:242890/125‑1 (MQ=255)                                   cGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCgg                                                                                                             >  2:242890/1‑125 (MQ=255)                                             ttGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTccac                                                                                    <  2:711826/140‑1 (MQ=255)                                              tGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTccacc                                                                                   >  1:898/1‑140 (MQ=255)                                                tCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAg                                                                                 >  1:1119749/1‑140 (MQ=255)                                                   cacGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCtt                                                                              >  1:415535/1‑140 (MQ=255)                                                     cGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCtttt                                                                            >  1:344971/1‑140 (MQ=255)                                                       gTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTtc                                                                          >  1:160844/1‑140 (MQ=255)                                                         tGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTtctc                                                                        <  1:1115039/140‑1 (MQ=255)                                                         tGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTtctc                                                                        <  2:861260/140‑1 (MQ=255)                                                           ctctTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTccc                                                                      >  1:722048/1‑140 (MQ=255)                                                              tttCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGt                                                                   >  1:726776/1‑140 (MQ=255)                                                                   ggCAGATCAAACCCAGCATTTTCAGCGTCACCCCATTGGCCCACCAAAAGCCATCCTGAAATGGGTATTCGCCCCCGCCGCCCCCCTTTCCGGTGGTGCTGACATCATTTTTTTCCCCCAGCTTTTTCCCCCGGTCAAAg                                                              <  2:722048/140‑1 (MQ=255)                                                                     cAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCtttt                                                                            >  1:1124706/1‑124 (MQ=255)                                                                     cAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCtttt                                                                            <  2:1124706/124‑1 (MQ=255)                                                                     cAGATCAAATCCAACATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGgt                                                            <  2:1193804/140‑1 (MQ=255)                                                                        aTCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGtgtg                                                         >  2:484956/1‑140 (MQ=255)                                                                                cAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACAt                                                 <  2:160844/140‑1 (MQ=255)                                                                                  gCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTg                                               >  1:853892/1‑140 (MQ=255)                                                                                   cATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTgg                                              >  1:11369/1‑140 (MQ=255)                                                                                    aTTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGt                                             <  2:415535/140‑1 (MQ=255)                                                                                            cGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGt                                     <  2:898/140‑1 (MQ=255)                                                                                             gTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTg                                    <  2:344971/140‑1 (MQ=255)                                                                                             gTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTg                                    <  2:703044/140‑1 (MQ=255)                                                                                             gTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTg                                    <  2:726776/140‑1 (MQ=255)                                                                                                 cGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAg                                >  2:384629/1‑140 (MQ=255)                                                                                                      ttGGTCCAGCCAAAGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAAt                           >  1:196413/1‑140 (MQ=255)                                                                                                                  aaGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCAc               <  2:11369/140‑1 (MQ=255)                                                                                                                   aGCCATCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCAcc              >  2:1093648/1‑140 (MQ=255)                                                                                                                        tCCTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCGGCTAATGTCCATCGCCACCTCttt         >  1:675031/1‑140 (MQ=255)                                                                                                                         ccTGTAATGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCtttt        >  1:343719/1‑140 (MQ=255)                                                                                                                               aTGGGTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCg  <  1:1195584/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCG  >  NC_000913/1245711‑1245976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑