Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,298,691	A→G	I32V (ATC→GTC)	ychE →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,298,691	0	A	G	100.0%	256.3 / NA	69	I32V (ATC→GTC)	ychE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/40);  total (29/40)

TTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAA  >  NC_000913/1298554‑1298823                                                                                                                                          |                                                                                                                                     ttatttatGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTc                                                                                                                                    >  2:980678/1‑140 (MQ=255)  tatttatGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCa                                                                                                                                   >  1:849758/1‑140 (MQ=255)   atttatTGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTc                                                                                                                                    >  2:656059/1‑138 (MQ=255)   atttatGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTc                                                                                                                                    <  1:656059/138‑1 (MQ=255)    tttatGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGc                                                                                                                                 <  1:255678/140‑1 (MQ=255)      tatGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCAt                                                                                                                               <  1:980678/140‑1 (MQ=255)      tatGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCAt                                                                                                                               <  2:351433/140‑1 (MQ=255)         ggCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTGGGGATTATTCCCGTATTTGTCAGCATGAc                                                                                                                            <  2:315921/140‑1 (MQ=255)         ggCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGAc                                                                                                                            <  2:12681/140‑1 (MQ=255)          gCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTTAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGAcc                                                                                                                           <  2:1216192/140‑1 (MQ=255)          gCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGAcc                                                                                                                           <  2:849758/140‑1 (MQ=255)          gCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGAcc                                                                                                                           <  2:580215/140‑1 (MQ=255)          gCTAACATTATCCGGCTTTTGCTTCGGAGCCAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCTGGTAGGGATTATTCCCGTCTTTGTCAGCATGAcc                                                                                                                           <  1:688792/140‑1 (MQ=255)            tAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAg                                                                                                                         <  2:459568/140‑1 (MQ=255)             aaCATTATCCGGCTTTTGCTTCGGAGCTATCCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGt                                                                                                                        >  1:1075601/1‑140 (MQ=255)              aCATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGAcc                                                                                                                           <  1:1067060/136‑1 (MQ=255)              aCATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATATGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGAcc                                                                                                                           >  2:1067060/1‑136 (MQ=255)               cATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTa                                                                                                                      >  1:258862/1‑140 (MQ=255)                aTTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTAt                                                                                                                     <  1:335280/140‑1 (MQ=255)                         cTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCg                                                                                                            >  1:432356/1‑140 (MQ=255)                          ttttGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGAc                                                                                                                            >  1:995006/1‑123 (MQ=255)                          ttttGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGAc                                                                                                                            <  2:995006/123‑1 (MQ=255)                          ttttGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCgg                                                                                                           <  2:972753/140‑1 (MQ=255)                               cTTCGGAGCTAACCGTGATTCAACCCTTTTTTGAATTTCCCGTTTACGTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAgcg                                                                                                      <  2:865973/140‑1 (MQ=255)                                       cTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACaa                                                                                              >  1:25331/1‑140 (MQ=255)                                               gATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCACGAAACAAAACTAAcc                                                                                      >  1:1064113/1‑140 (MQ=255)                                                  tCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTa                                                                                   <  2:25331/140‑1 (MQ=255)                                                    aGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTa                                                                                         >  1:364078/1‑132 (MQ=255)                                                    aGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTa                                                                                         <  2:364078/132‑1 (MQ=255)                                                    aGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACa                                                                                 <  2:23355/140‑1 (MQ=255)                                                      aCCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGc                                                                               <  2:258862/140‑1 (MQ=255)                                                      aCCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGc                                                                               >  2:353348/1‑140 (MQ=255)                                                      aCCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGc                                                                               >  1:731832/1‑140 (MQ=255)                                                      aCCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGc                                                                               <  1:557618/140‑1 (MQ=255)                                                      aCCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGc                                                                               <  1:147376/140‑1 (MQ=255)                                                      aCCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGc                                                                               <  1:1126136/140‑1 (MQ=255)                                                           ttttGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACAAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAAcc                                                                          <  1:434596/140‑1 (MQ=255)                                                                     cccGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGc                                                                >  2:447842/1‑140 (MQ=255)                                                                      ccGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGcc                                                               <  1:447842/140‑1 (MQ=255)                                                                        gTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCAt                                                             <  1:39678/140‑1 (MQ=255)                                                                         tttACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCAtt                                                            <  2:357528/140‑1 (MQ=255)                                                                         tttACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCAtt                                                            <  2:151741/140‑1 (MQ=255)                                                                              ttCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCtt                                                       >  2:949131/1‑140 (MQ=255)                                                                                  aaTTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTgg                                                   >  2:316760/1‑140 (MQ=255)                                                                                    tttttCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGAt                                                 <  2:1075601/140‑1 (MQ=255)                                                                                        tCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCg                                             >  2:350363/1‑140 (MQ=255)                                                                                        tCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCg                                             >  2:714675/1‑140 (MQ=255)                                                                                        tCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCg                                             >  1:1060970/1‑140 (MQ=255)                                                                                                 tATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTtctc                                    <  2:688621/140‑1 (MQ=255)                                                                                                         cTGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGacac                            >  2:1044086/1‑140 (MQ=255)                                                                                                          tGGTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACg                           <  1:214526/140‑1 (MQ=255)                                                                                                            gTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGAt                         >  1:26590/1‑140 (MQ=255)                                                                                                            gTCAACCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGAt                         >  2:1004884/1‑140 (MQ=255)                                                                                                                aCCCGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTa                     <  1:1004884/140‑1 (MQ=255)                                                                                                                 cccGGTAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTAc                    >  1:957448/1‑140 (MQ=255)                                                                                                                      tAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAACACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGa                               <  2:1092983/124‑1 (MQ=255)                                                                                                                      tAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGa                               >  1:1092983/1‑124 (MQ=255)                                                                                                                      tAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACtt               <  1:714675/140‑1 (MQ=255)                                                                                                                      tAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACtt               >  2:302604/1‑140 (MQ=255)                                                                                                                      tAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACtt               <  1:350363/140‑1 (MQ=255)                                                                                                                      tAGGGATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACtt               >  1:1190881/1‑140 (MQ=255)                                                                                                                         ggATTATTCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACttttt            <  1:808123/140‑1 (MQ=255)                                                                                                                           attattCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTgg          >  2:558480/1‑140 (MQ=255)                                                                                                                           attattCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTgg          >  1:277872/1‑140 (MQ=255)                                                                                                                            ttattCCCGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGt         <  2:1190881/140‑1 (MQ=255)                                                                                                                                   cGTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCaa  <  1:425089/140‑1 (MQ=255)                                                                                                                                    gTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCaa  >  1:157618/1‑139 (MQ=255)                                                                                                                                    gTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCaa  <  2:797079/139‑1 (MQ=255)                                                                                                                                    gTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCaa  >  1:797079/1‑139 (MQ=255)                                                                                                                                    gTCTTTGTCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCaa  <  2:157618/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TTATTTATGGCTAACATTATCCGGCTTTTGCTTCGGAGCTAACCGTGATTCAGACCTTTTTTGATTTTCCCGTTTACTTCAAATTTTTCATCGGGTTATTTGCGCTGGTCAACCCGGTAGGGATTATTCCCGTCTTTATCAGCATGACCAGTTATCAGACAGCGGCAGCGCGAAACAAAACTAACCTTACAGCCAACCTGTCTGTGGCCATTATCTTGTGGATCTCGCTTTTTCTCGGCGACACGATTCTACAACTTTTTGGTATATCAA  >  NC_000913/1298554‑1298823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑