Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,327,734	T→C	intergenic (+7/+33)	rluB → / ← btuR	23S rRNA pseudouridine(2605) synthase/cobinamide/cobalamin adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,327,734	0	T	C	100.0%	105.5 / NA	31	intergenic (+7/+33)	rluB/btuR	23S rRNA pseudouridine(2605) synthase/cobinamide/cobalamin adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/23);  total (8/23)

CCAGCTCTAAAGTCGCTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGTATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCC  >  NC_000913/1327600‑1327863                                                                                                                                       |                                                                                                                                  ccAGCTCTAAAGTCGCTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGGAATAATAAAGGGTAATCAAAAAATGcc                                                                                                                              >  2:292027/1‑140 (MQ=255)  cAGCTCTAAAGTCGCTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCg                                                                                                                             <  1:500254/140‑1 (MQ=255)  cAGCTCTAAAGTCGCTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCg                                                                                                                             <  2:913029/140‑1 (MQ=255)   aGCTCTAAAGTCGCTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCgg                                                                                                                            <  1:489578/140‑1 (MQ=255)               gCTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGGCGTAATAAAAAAGGTTAATCAAAACAAGCCGGACGGCATATCCg                                                                                                                >  2:846711/1‑140 (MQ=255)               gCTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGGCGTTTTGGCGGGCGTAAAAAAAACGGGTAATTAAAGCATGCCGGGCGTCATATTCg                                                                                                                >  2:547699/1‑140 (MQ=255)                cTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCgg                                                                                                               <  1:648034/140‑1 (MQ=255)                        aaaGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTa                                                                                                       <  2:795382/140‑1 (MQ=255)                         aaGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTAc                                                                                                      <  2:565762/140‑1 (MQ=255)                         aaGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTAc                                                                                                      <  1:846711/140‑1 (MQ=255)                          aGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACa                                                                                                     <  2:477684/140‑1 (MQ=255)                             ccGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGAt                                                                                                  >  1:810153/1‑140 (MQ=255)                                tcgtcgCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTa                                                                                                       >  2:861370/1‑132 (MQ=255)                                tcgtcgCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTa                                                                                                       <  1:861370/132‑1 (MQ=255)                                tcgtcgCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATtaa                                                                                               <  1:356204/140‑1 (MQ=255)                                                 aTCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTg                                                                              <  1:80857/140‑1 (MQ=255)                                                           tcgtGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGCGGCTCTTGCGGGAGTAAAAAAAAAGGCTAAAAAAAACAAGCCGGGCGCGCTATTCCGGACTTTTTCAGATTAATAATCAAACCCTATTTGCGCTTTTaca                                                                    >  2:72554/1‑140 (MQ=255)                                                               gCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCgg                                                                <  1:400773/140‑1 (MQ=255)                                                                        cGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACg                                                       <  2:936218/140‑1 (MQ=255)                                                                          tCACAGTCAGGTGAGCGGCGGGCGTCGTTCTTGGGGGCGGAAAAATAACGGGTAATCAAAACATGCCGGGCGGCATATTCCGCCTTTTTTTAAATTTATAATCGATCCCCATCTTCGCTTTTTCACCCGCATCaaaacca                                                     >  2:944171/1‑136 (MQ=255)                                                                                  aGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGAc                                             >  1:707197/1‑140 (MQ=255)                                                                                       aGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGc                                        <  1:729987/140‑1 (MQ=255)                                                                                          ggcggTCGTCGTTCTGGCGGACGGAATAATAACGGGTAATCAAAGCATGCCGGGCGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCCGCATCAAACGCATGTTTTACGGGGCgga                                     >  2:664010/1‑138 (MQ=255)                                                                                           gcggTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTaa                                    <  1:314571/140‑1 (MQ=255)                                                                                            cggTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAAt                                   >  1:782718/1‑140 (MQ=255)                                                                                             ggTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAAtt                                  <  2:782718/140‑1 (MQ=255)                                                                                              gtcgtcGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACg                                            >  2:103248/1‑129 (MQ=255)                                                                                              gtcgtcGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACg                                            <  1:103248/129‑1 (MQ=255)                                                                                              gtcgtcGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGt                                      <  2:32671/135‑1 (MQ=255)                                                                                              gtcgtcGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGt                                      >  1:32671/1‑135 (MQ=255)                                                                                              gtcgtcGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTaa                                    <  2:1186894/137‑1 (MQ=255)                                                                                              gtcgtcGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTaa                                    >  1:1186894/1‑137 (MQ=255)                                                                                                   cgtTCTGGCGGACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTa                            <  1:547699/140‑1 (MQ=255)                                                                                                             gACGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGc                  <  1:664010/140‑1 (MQ=255)                                                                                                              aCGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTGATTCACTTACCGTATCTGcc                 <  2:707197/140‑1 (MQ=255)                                                                                                               cGTAATAATAACGGTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCa                >  2:647353/1‑140 (MQ=255)                                                                                                                         actgTTAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAat      <  1:753788/137‑1 (MQ=255)                                                                                                                             ttAATCAAAGCATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATcc  >  2:844639/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  CCAGCTCTAAAGTCGCTGTAGAAAAAGACCGTCGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGCGGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAATCAAAGTATGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCC  >  NC_000913/1327600‑1327863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑