Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,453,613	A→G	G10G (GGT→GGC)	paaZ ←	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,453,613	0	A	G	100.0%	308.9 / NA	85	G10G (GGT→GGC)	paaZ	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/49);  total (36/49)

GCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATC  >  NC_000913/1453479‑1453739                                                                                                                                        |                                                                                                                               gggggggCCCTTTTTTAAATGCAAACCGGGCGGGGG‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGa                                                                                                                           <  2:460992/138‑1 (MQ=255)  ggggggCCCCTTTTTCAATGGCAAACGGGCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGAt                                                                                                                          <  2:486381/139‑1 (MQ=255)    ggggCACCTTTTTCAATGGCAAACTGGCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATaa                                                                                                                        >  1:578347/1‑140 (MQ=255)      ggCCCCTTTTTTAAAGGGAAACCGGGGGGGAGGCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTG‑CAGGTGCCGGATAAGa                                                                                                                      <  1:948843/140‑1 (MQ=255)          ccTTTTTCAATGGCAAACTGGCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACt                                                                                                                  >  1:198420/1‑140 (MQ=255)                    gggCAAACGGGCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAActgc                                                                                                        <  1:576268/139‑1 (MQ=255)                      gCAAACTGGCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAActgctg                                                                                                      <  1:204059/140‑1 (MQ=255)                      gCAAACTGGCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAActgctg                                                                                                      <  1:504028/140‑1 (MQ=255)                        aaaCTGGCGGGCA‑CCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCa                                                                                                    >  2:591088/1‑140 (MQ=255)                         accTGGGGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCAt                                                                                                   <  2:231718/138‑1 (MQ=255)                            gggggggCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGc                                                                                                <  2:664154/136‑1 (MQ=255)                              gCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTa                                                                                              <  2:1041831/140‑1 (MQ=255)                                gggCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCt                                                                                               <  1:1001909/137‑1 (MQ=255)                                gggCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCt                                                                                               >  2:1001909/1‑137 (MQ=255)                                   cA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTa                                                                                              <  1:286629/135‑1 (MQ=255)                                   cA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTa                                                                                              >  2:286629/1‑135 (MQ=255)                                       ccgccATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTa                                                                                              >  2:297995/1‑132 (MQ=255)                                       ccgccATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTa                                                                                              <  1:297995/132‑1 (MQ=255)                                         gccATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGAt                                                                                    >  1:225774/1‑140 (MQ=255)                                            atatCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGtt                                                                                 <  1:1024711/140‑1 (MQ=255)                                             tatCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGttt                                                                                <  2:500064/140‑1 (MQ=255)                                                  aGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACAt                                                                           >  1:1078663/1‑140 (MQ=255)                                                    aCCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACAttt                                                                         <  1:393847/140‑1 (MQ=255)                                                       ttCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTg                                                                      >  2:549714/1‑140 (MQ=255)                                                        tCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGtt                                                                                 <  2:198930/128‑1 (MQ=255)                                                        tCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGtt                                                                                 >  1:198930/1‑128 (MQ=255)                                                        tCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGt                                                                     >  2:994697/1‑140 (MQ=255)                                                         cACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGtt                                                                    >  2:894745/1‑140 (MQ=255)                                                         cACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGtt                                                                    >  2:466964/1‑140 (MQ=255)                                                         cACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGtt                                                                    >  2:377601/1‑140 (MQ=255)                                                            tGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt                                                                 <  1:861339/140‑1 (MQ=255)                                                            tGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt                                                                 <  2:1078663/140‑1 (MQ=255)                                                              gTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt                                                                 <  2:784361/138‑1 (MQ=255)                                                              gTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt                                                                 >  1:784361/1‑138 (MQ=255)                                                              gTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTaa                                                               >  2:474882/1‑140 (MQ=255)                                                               tCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAAt                                                              >  2:836045/1‑140 (MQ=255)                                                                    tCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt                                                                 >  1:697806/1‑132 (MQ=255)                                                                    tCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCt                                                                 <  2:697806/132‑1 (MQ=255)                                                                        aTAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATc                                                     >  1:175888/1‑140 (MQ=255)                                                                             gCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGaa                                                <  1:836045/140‑1 (MQ=255)                                                                               cTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATc                                              >  2:735170/1‑140 (MQ=255)                                                                               cTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATc                                              >  1:1085437/1‑140 (MQ=255)                                                                               cTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATc                                              >  2:1004382/1‑140 (MQ=255)                                                                                  gccgcTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATcgc                                                   <  1:591822/132‑1 (MQ=255)                                                                                  gccgcTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATcgc                                                   >  2:591822/1‑132 (MQ=255)                                                                                    cgcTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATaaa                                         <  2:744802/140‑1 (MQ=255)                                                                                    cgcTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATaaa                                         <  1:721029/140‑1 (MQ=255)                                                                                              gtggtgAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGa                               >  1:768958/1‑140 (MQ=255)                                                                                                ggtgAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATc                             >  2:434272/1‑140 (MQ=255)                                                                                                 gtgAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCt                            >  1:322621/1‑140 (MQ=255)                                                                                                  tgAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCtt                           >  1:470126/1‑140 (MQ=255)                                                                                                      tCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTaa                       <  1:591088/140‑1 (MQ=255)                                                                                                        aaaCGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACg                     <  1:377601/140‑1 (MQ=255)                                                                                                        aaaCGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACg                     <  1:434272/140‑1 (MQ=255)                                                                                                        aaaCGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACg                     <  1:474882/140‑1 (MQ=255)                                                                                                          aCGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGaa                   <  1:994697/140‑1 (MQ=255)                                                                                                          aCGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGaa                   >  1:906512/1‑140 (MQ=255)                                                                                                           cGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGaaa                  >  1:747514/1‑140 (MQ=255)                                                                                                           cGGCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGaaa                  >  1:577561/1‑140 (MQ=255)                                                                                                            ggCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAAt                 >  2:738561/1‑140 (MQ=255)                                                                                                            ggCTACGGCCCCGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAAt                 >  1:485353/1‑140 (MQ=255)                                                                                                                     cccGGCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTAt        <  2:768958/140‑1 (MQ=255)                                                                                                                        ggCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGa                    >  2:1084561/1‑125 (MQ=255)                                                                                                                        ggCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGa                    <  1:1084561/125‑1 (MQ=255)                                                                                                                        ggCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCg     <  2:906512/140‑1 (MQ=255)                                                                                                                        ggCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCg     <  1:466964/140‑1 (MQ=255)                                                                                                                        ggCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCg     <  2:995282/140‑1 (MQ=255)                                                                                                                         gCCAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGa    <  1:305463/140‑1 (MQ=255)                                                                                                                          ccAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGAt   <  1:1195070/140‑1 (MQ=255)                                                                                                                          ccAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGAt   <  2:747514/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  1:1004382/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  2:485353/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  2:470126/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  2:322621/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  2:225774/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  1:894745/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  1:738561/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  1:549714/140‑1 (MQ=255)                                                                                                                           cAGACTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  1:5145/140‑1 (MQ=255)                                                                                                                              aCTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGa    <  2:341843/135‑1 (MQ=255)                                                                                                                              aCTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGa    >  1:341843/1‑135 (MQ=255)                                                                                                                              aCTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  >  1:977679/1‑137 (MQ=255)                                                                                                                              aCTGCCAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  2:977679/137‑1 (MQ=255)                                                                                                                                  ccAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  >  1:937928/1‑133 (MQ=255)                                                                                                                                  ccAGGTGCCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATc  <  2:937928/133‑1 (MQ=255)                                                                                                                                        |                                                                                                                               GCGGGGGCACCTTTTTCAATGGCAAACTGGCGGGCA‑GCCGCCATATCAAGACCTTCACTGGTCACTTCCCATAACGCCTCGCCGCTAATAGCGTGGTGAATCAAACGGCTACGGCCCCGGCCAGACTGCCAGGTACCGGATAAGAAACTGGCTAACTGCTGCATCGCTACTCTCCAGATGTTTCACATTTCTGTTGCTAATAGTTAAATCGCGAATCATAAAAAGCAAAGGATCTTTTAACGAAATGTTAACTATGCGATC  >  NC_000913/1453479‑1453739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑