Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,464,087	T→C	R183R (CGT→CGC)	paaX →	DNA‑binding transcriptional repressor PaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,464,087	0	T	C	100.0%	139.6 / NA	40	R183R (CGT→CGC)	paaX	DNA‑binding transcriptional repressor PaaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/22);  total (18/22)

CTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATA  >  NC_000913/1463950‑1464220                                                                                                                                          |                                                                                                                                      cTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTgcgcgc                                                                                                                                     <  1:415191/140‑1 (MQ=255) cTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTgcgcgc                                                                                                                                     >  2:163847/1‑140 (MQ=255)     cACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCaga                                                                                                                                 >  1:360739/1‑140 (MQ=255)          aGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTaga                                                                                                                            >  1:328617/1‑140 (MQ=255)           gCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGGACAGACACTTTTGCATGAAGCGGGTGTGGCGGGGAACGCGATTTTTTTTGCAACGCAAAAACCACCGGCGCTTTCTCGCGCAGCACTGCGCGCCACCGTagaa                                                                                                                           >  2:415191/1‑140 (MQ=255)            ccTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTagaag                                                                                                                          >  1:622665/1‑140 (MQ=255)            ccTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTagaa                                                                                                                           <  2:436785/139‑1 (MQ=255)            ccTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTagaa                                                                                                                           >  1:436785/1‑139 (MQ=255)                 tGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGc                                                                                                                     <  1:886981/140‑1 (MQ=255)                 tGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGc                                                                                                                     <  2:360739/140‑1 (MQ=255)                     aTCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGc                                                                                                                 >  1:866512/1‑140 (MQ=255)                      tCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTTTTTTTAAGCGCAAATACCACTGGCGCTTTCTCGCGCCGCACTGCGCGGCAGAGCAGAAGAGTGCTGGCa                                                                                                                >  2:368821/1‑140 (MQ=255)                      tCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCa                                                                                                                <  2:622665/140‑1 (MQ=255)                           gTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTaa                                                                                                           <  2:328617/140‑1 (MQ=255)                            tCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAAc                                                                                                          >  1:556291/1‑140 (MQ=255)                                          cGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa                                                                                            >  2:84219/1‑140 (MQ=255)                                                 cAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGa                                                                                     <  2:14160/140‑1 (MQ=255)                                                   gACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGaaa                                                                                   <  1:163847/140‑1 (MQ=255)                                                      acTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCt                                                                                <  2:556291/140‑1 (MQ=255)                                                        ttttGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCttt                                                                              >  2:936312/1‑140 (MQ=255)                                                        ttttGCATGAAGCGGGTGTGGCGGATAACGGGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCttt                                                                              >  1:766160/1‑140 (MQ=255)                                                         tttGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTa                                                                             <  2:779585/140‑1 (MQ=255)                                                            gCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTc                                                                          <  1:1038049/140‑1 (MQ=255)                                                                                aTAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTg                                                      >  1:622772/1‑140 (MQ=255)                                                                                aTAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTg                                                      >  1:593483/1‑140 (MQ=255)                                                                                 tAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGc                                                     <  2:593483/140‑1 (MQ=255)                                                                                  aaCGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGcc                                                    <  1:936312/140‑1 (MQ=255)                                                                                    cGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGc                                                  <  2:940746/140‑1 (MQ=255)                                                                                          ttGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTaa                                            >  1:886532/1‑140 (MQ=255)                                                                                             ttttGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAag                                         <  2:359551/140‑1 (MQ=255)                                                                                             ttttGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAag                                         >  2:250913/1‑140 (MQ=255)                                                                                                tGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAgg                                      <  2:622772/140‑1 (MQ=255)                                                                                                       cAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                               <  1:368821/140‑1 (MQ=255)                                                                                                       cAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                               <  1:250913/140‑1 (MQ=255)                                                                                                       cAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                               >  2:1071389/1‑140 (MQ=255)                                                                                                            aCCACTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGt                          <  1:84219/140‑1 (MQ=255)                                                                                                               aCTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTaa                       >  2:1193266/1‑140 (MQ=255)                                                                                                               aCTGGCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTaa                       <  2:181769/140‑1 (MQ=255)                                                                                                                  ggCGCTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAAccc                    >  1:59161/1‑140 (MQ=255)                                                                                                                    cgcTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTaa                       >  2:596678/1‑135 (MQ=255)                                                                                                                    cgcTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTaa                       <  1:596678/135‑1 (MQ=255)                                                                                                                      cTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGa                >  1:331732/1‑140 (MQ=255)                                                                                                                      cTTTCTCGCGCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGa                <  2:979402/140‑1 (MQ=255)                                                                                                                            cgcgCAGCACTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGc          <  2:866512/140‑1 (MQ=255)                                                                                                                                    aCTGCGCGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCata  >  2:702808/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATA  >  NC_000913/1463950‑1464220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑