Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,563,067	T→C	T4A (ACC→GCC)	ddpX ←	D‑alanyl‑D‑alanine dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,563,067	0	T	C	100.0%	268.5 / NA	74	T4A (ACC→GCC)	ddpX	D‑alanyl‑D‑alanine dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/38);  total (36/38)

GCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTG  >  NC_000913/1562931‑1563201                                                                                                                                         |                                                                                                                                       gcgGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATc                                                                                                                                     <  2:908171/140‑1 (MQ=255)  cgGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATcc                                                                                                                                    <  2:1147057/140‑1 (MQ=255)          gCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATaaa                                                                                                                            >  2:57877/1‑140 (MQ=255)           cATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAAc                                                                                                                           <  2:715133/140‑1 (MQ=255)           cATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAAc                                                                                                                           <  2:96546/140‑1 (MQ=255)           cATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAAc                                                                                                                           <  1:1074990/140‑1 (MQ=255)           cATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAAc                                                                                                                           <  1:1059442/140‑1 (MQ=255)              ccTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGt                                                                                                                        <  2:480416/140‑1 (MQ=255)              ccTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGt                                                                                                                        <  2:433547/140‑1 (MQ=255)               cTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAAc                                                                                                                           >  1:187138/1‑136 (MQ=255)               cTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAAc                                                                                                                           <  2:187138/136‑1 (MQ=255)               cTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGtt                                                                                                                       >  1:232674/1‑140 (MQ=255)                ttGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTc                                                                                                                      <  1:1188822/140‑1 (MQ=255)                   tgtAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATaaa                                                                                                                            <  1:886541/131‑1 (MQ=255)                   tgtAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATaaa                                                                                                                            >  2:886541/1‑131 (MQ=255)                      aaCAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGtat                                                                                                                <  1:220667/140‑1 (MQ=255)                      aaCAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGtat                                                                                                                >  2:344054/1‑140 (MQ=255)                          gACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGa                                                                                                            >  2:898910/1‑140 (MQ=255)                           aCAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGAc                                                                                                           >  2:88772/1‑140 (MQ=255)                             aaCGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGt                                                                                                                        <  1:839423/125‑1 (MQ=255)                             aaCGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGt                                                                                                                        >  2:839423/1‑125 (MQ=255)                             aaCGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACaa                                                                                                         >  2:1059018/1‑140 (MQ=255)                               cgcgCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGtat                                                                                                                <  1:430261/131‑1 (MQ=255)                               cgcgCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGtat                                                                                                                >  2:430261/1‑131 (MQ=255)                               cgcgCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACaaaa                                                                                                       >  2:780945/1‑140 (MQ=255)                               cgcgCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACaaaa                                                                                                       >  1:719230/1‑140 (MQ=255)                               cgcgCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACaaaa                                                                                                       >  1:245415/1‑140 (MQ=255)                                     tGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATtgct                                                                                                 <  2:390288/140‑1 (MQ=255)                                     tGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATtgct                                                                                                 >  2:440577/1‑140 (MQ=255)                                           tAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCtt                                                                                           <  1:344054/140‑1 (MQ=255)                                              aTAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATtgctg                                                                                                <  1:1109933/132‑1 (MQ=255)                                              aTAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATtgctg                                                                                                >  2:1109933/1‑132 (MQ=255)                                              aTAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTcc                                                                                        <  1:898910/140‑1 (MQ=255)                                                aGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGc                                                                                      <  1:571818/140‑1 (MQ=255)                                                aGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGc                                                                                      <  1:499565/140‑1 (MQ=255)                                                  cTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCtt                                                                                    >  1:1046510/1‑140 (MQ=255)                                                    ttACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTAt                                                                                  >  1:408787/1‑140 (MQ=255)                                                    ttACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTAt                                                                                  >  2:855007/1‑140 (MQ=255)                                                    ttACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTAt                                                                                  >  1:730630/1‑140 (MQ=255)                                                      aCCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGc                                                                                <  2:1046510/140‑1 (MQ=255)                                                       ccTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCa                                                                               <  1:855007/140‑1 (MQ=255)                                                       ccTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGACCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCa                                                                               <  2:726483/140‑1 (MQ=255)                                                            gATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGAtct                                                                          >  2:195234/1‑140 (MQ=255)                                                                ttATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatg                                                                      <  1:1059018/140‑1 (MQ=255)                                                                  aTCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgcc                                                                    <  2:353309/140‑1 (MQ=255)                                                                  aTCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgcc                                                                    >  2:972165/1‑140 (MQ=255)                                                                     aGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgccatg                                                                 >  1:985970/1‑140 (MQ=255)                                                                       cgcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCatgccatgcc                                                               >  2:596124/1‑140 (MQ=255)                                                                        gcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCg                                                              <  2:259436/140‑1 (MQ=255)                                                                        gcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCg                                                              <  2:569574/140‑1 (MQ=255)                                                                          aGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCggg                                                            >  1:617256/1‑140 (MQ=255)                                                                            gCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCTGGGAt                                                          >  2:357879/1‑140 (MQ=255)                                                                            gCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGAt                                                          <  1:57877/140‑1 (MQ=255)                                                                            gCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGAt                                                          <  1:780945/140‑1 (MQ=255)                                                                             cGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCTGGGATa                                                         <  1:357879/140‑1 (MQ=255)                                                                                      aTTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGa                                                >  1:400305/1‑140 (MQ=255)                                                                                         cGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                             <  2:985970/140‑1 (MQ=255)                                                                                         cGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                             <  2:617256/140‑1 (MQ=255)                                                                                         cGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                             <  2:513695/140‑1 (MQ=255)                                                                                         cGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                             <  1:972165/140‑1 (MQ=255)                                                                                           aTCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCCGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCt                                           >  2:442303/1‑140 (MQ=255)                                                                                               ccAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCt                                       <  1:596124/140‑1 (MQ=255)                                                                                                        ggAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAGTTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTtat                              >  2:642291/1‑140 (MQ=255)                                                                                                        ggAAGATTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTtat                              >  1:499548/1‑140 (MQ=255)                                                                                                             aTTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtttt                         >  2:851546/1‑140 (MQ=255)                                                                                                             aTTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtttt                         >  1:218269/1‑140 (MQ=255)                                                                                                             aTTACGGCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtttt                         >  2:881452/1‑140 (MQ=255)                                                                                                                  ggCTAAATCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAAc                    >  2:63510/1‑140 (MQ=255)                                                                                                                       aaTCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACttt                 <  1:783461/138‑1 (MQ=255)                                                                                                                       aaTCAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACttt                 >  2:783461/1‑138 (MQ=255)                                                                                                                          cAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATc            <  2:1179607/140‑1 (MQ=255)                                                                                                                          cAACCAGTTCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATc            <  1:442303/140‑1 (MQ=255)                                                                                                                                 ttCGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACAttt     >  2:1171863/1‑140 (MQ=255)                                                                                                                                   cGGTGGCATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTtgt   >  1:95721/1‑140 (MQ=255)                                                                                                                                    ggtggcATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTtgtg  <  1:88772/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTGTG  >  NC_000913/1562931‑1563201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑