Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,524	T→C	V114A (GTT→GCT)	speG →	spermidine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,524	0	T	C	100.0%	280.3 / NA	76	V114A (GTT→GCT)	speG	spermidine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/49);  total (27/49)

GGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAA  >  NC_000913/1656391‑1656656                                                                                                                                      |                                                                                                                                     ggTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCaa                                                                                                                                <  2:801830/140‑1 (MQ=255) ggTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCaa                                                                                                                                <  2:287997/140‑1 (MQ=255) ggTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCaa                                                                                                                                <  2:1098321/140‑1 (MQ=255) ggTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCaa                                                                                                                                <  1:693544/140‑1 (MQ=255) ggTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCaa                                                                                                                                <  1:91308/140‑1 (MQ=255) ggTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCaa                                                                                                                                <  2:425137/140‑1 (MQ=255)  gTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAAt                                                                                                                               <  1:937279/140‑1 (MQ=255)   tCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAAtc                                                                                                                              <  1:146795/140‑1 (MQ=255)   tCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCCCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAAtc                                                                                                                              <  2:60879/140‑1 (MQ=255)       gtggAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCta                                                                                                                          <  2:897340/140‑1 (MQ=255)       gtggAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCta                                                                                                                          >  2:1053010/1‑140 (MQ=255)        tggAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCtat                                                                                                                         <  2:447832/140‑1 (MQ=255)         ggAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCtata                                                                                                                        <  1:1132478/140‑1 (MQ=255)         ggAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCtata                                                                                                                        >  2:1201529/1‑140 (MQ=255)           ggCTGGTGGAAATTAACCATGTTCATCGGCGCGGAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAg                                                                                                                      <  2:773867/139‑1 (MQ=255)           aGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAg                                                                                                                      <  2:489547/140‑1 (MQ=255)           aGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAg                                                                                                                      <  1:1053010/140‑1 (MQ=255)           aGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAg                                                                                                                      <  2:65114/140‑1 (MQ=255)             cTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCt                                                                                                                    >  1:1092189/1‑140 (MQ=255)             cTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCt                                                                                                                    >  1:1197821/1‑140 (MQ=255)                 tggAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACAGCTCTCAATCTCtat                                                                                                                         <  2:963995/131‑1 (MQ=255)                 tggAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACAGCTCTCAATCTCtat                                                                                                                         >  1:963995/1‑131 (MQ=255)                      aTTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGAt                                                                                                           <  1:117571/140‑1 (MQ=255)                        tAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCg                                                                                                         <  2:716836/140‑1 (MQ=255)                        tAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGACGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCg                                                                                                         <  2:1092189/140‑1 (MQ=255)                                ttCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATaaa                                                                                                 <  1:843817/140‑1 (MQ=255)                                     cgccgcGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATaaa                                                                                                 >  2:816908/1‑135 (MQ=255)                                     cgccgcGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATaaa                                                                                                 <  1:816908/135‑1 (MQ=255)                                     cgccgcGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATa                                                                                                   <  2:1039090/133‑1 (MQ=255)                                     cgccgcGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATa                                                                                                   >  1:1039090/1‑133 (MQ=255)                                      gccgcGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAAt                                                                                           >  2:800179/1‑140 (MQ=255)                                      gccgcGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAAt                                                                                           >  2:367128/1‑140 (MQ=255)                                          cgcAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAaga                                                                                               <  1:38886/132‑1 (MQ=255)                                          cgcAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAaga                                                                                               >  2:38886/1‑132 (MQ=255)                                            cAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGAGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGaaaaa                                                                                     <  1:367128/140‑1 (MQ=255)                                             aGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAg                                                                                    <  1:95059/140‑1 (MQ=255)                                                aTTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGa                                                                                 <  1:479688/140‑1 (MQ=255)                                                         aaTTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGAt                                                                                <  1:577296/132‑1 (MQ=255)                                                         aaTTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGAt                                                                                >  2:577296/1‑132 (MQ=255)                                                           ttATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTAc                                                                      >  2:980043/1‑140 (MQ=255)                                                                          aTCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCttt                                                       >  1:626742/1‑140 (MQ=255)                                                                           tCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCtttt                                                      >  2:935597/1‑140 (MQ=255)                                                                            cAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTc                                                     >  1:1033708/1‑140 (MQ=255)                                                                               gggAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGt                                                  <  1:844883/140‑1 (MQ=255)                                                                                ggAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGtt                                                 <  1:1201529/140‑1 (MQ=255)                                                                                   aaGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGaa                                              >  1:1137448/1‑140 (MQ=255)                                                                                   aaGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGaa                                              >  1:274477/1‑140 (MQ=255)                                                                                   aaGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGaa                                              >  2:712513/1‑140 (MQ=255)                                                                                    aGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAg                                             <  2:626742/140‑1 (MQ=255)                                                                                                  gTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCAc                               <  1:712513/140‑1 (MQ=255)                                                                                                   tGCCGCCAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACg                              >  1:360153/1‑140 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAAt                                       <  2:860064/129‑1 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAAt                                       <  2:820085/129‑1 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAAt                                       >  1:860064/1‑129 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAg                            >  1:1030495/1‑140 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAg                            <  1:377272/140‑1 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAg                            >  2:451232/1‑140 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAg                            <  1:980043/140‑1 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAg                            >  2:138131/1‑140 (MQ=255)                                                                                                     ccgccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGCATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAAt                                       >  1:820085/1‑129 (MQ=255)                                                                                                       gccAAATTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGtt                          <  2:789863/140‑1 (MQ=255)                                                                                                          aaaTTAGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCtt                       >  2:696303/1‑140 (MQ=255)                                                                                                               aGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTttatta                  <  2:360974/140‑1 (MQ=255)                                                                                                               aGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTttatta                  <  2:1182565/140‑1 (MQ=255)                                                                                                               aGCAATGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTttatta                  <  1:138131/140‑1 (MQ=255)                                                                                                                    tGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGt             <  1:1160565/140‑1 (MQ=255)                                                                                                                    tGGACTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGt             <  2:274477/140‑1 (MQ=255)                                                                                                                       aCTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCaa          <  1:800179/140‑1 (MQ=255)                                                                                                                       aCTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCaa          <  2:1030495/140‑1 (MQ=255)                                                                                                                       aCTATGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCaa          <  1:935597/140‑1 (MQ=255)                                                                                                                           tGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAat         <  1:1067434/137‑1 (MQ=255)                                                                                                                           tGGCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAat         >  2:1067434/1‑137 (MQ=255)                                                                                                                            ggCTTTACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCg     <  1:1140888/140‑1 (MQ=255)                                                                                                                               tttACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTaa  <  1:283319/140‑1 (MQ=255)                                                                                                                                 tACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTttatta                  >  1:318793/1‑122 (MQ=255)                                                                                                                                 tACCGCTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTttatta                  <  2:318793/122‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GGTCTGGTGGAGCTGGTGGAAATTAACCATGTTCATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAGTATCAGGGGAAAGGTCTGGCAACCCGTGCCGCCAAATTAGCAATGGACTATGGCTTTACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTTGATAAAGAGAATGAAAAAGCGATTCACATTTACCGCAAGCTTGGCTTTTCGGTTGAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAA  >  NC_000913/1656391‑1656656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑