Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,741,941	G→A	G177S (GGC→AGC)	cfa →	cyclopropane fatty acyl phospholipid synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,741,941	0	G	A	100.0%	233.6 / NA	65	G177S (GGC→AGC)	cfa	cyclopropane fatty acyl phospholipid synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/32);  total (33/32)

TTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCGGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGAT  >  NC_000913/1741804‑1742075                                                                                                                                          |                                                                                                                                       ttGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGc                                                                                                                                      <  1:56674/140‑1 (MQ=255)        cTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCg                                                                                                                               <  2:106451/140‑1 (MQ=255)         ttCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCgg                                                                                                                              >  1:1051896/1‑140 (MQ=255)         ttCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCgg                                                                                                                              >  1:619879/1‑140 (MQ=255)         ttCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCgg                                                                                                                              >  1:13999/1‑140 (MQ=255)          tCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGa                                                                                                                             >  2:47926/1‑140 (MQ=255)                aaTATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGca                                                                                                                       <  2:957058/140‑1 (MQ=255)                   atTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCacac                                                                                                                    <  2:987146/140‑1 (MQ=255)                      ccTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGTAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTAc                                                                                                                 >  1:1118893/1‑140 (MQ=255)                       cTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACa                                                                                                                <  1:139440/140‑1 (MQ=255)                       cTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACa                                                                                                                <  1:365673/140‑1 (MQ=255)                          cgcTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGca                                                                                                                       <  2:808630/130‑1 (MQ=255)                          cgcTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGca                                                                                                                       >  1:808630/1‑130 (MQ=255)                           gcTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGTAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGc                                                                                                            <  2:1118893/140‑1 (MQ=255)                            cTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCa                                                                                                           <  1:153759/140‑1 (MQ=255)                             ttACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCAt                                                                                                          <  1:775201/140‑1 (MQ=255)                              tACTGGAAAGATGTCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATc                                                                                                         >  2:346799/1‑140 (MQ=255)                              tACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGc                                                                                                                        >  2:203794/1‑125 (MQ=255)                              tACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGc                                                                                                                        <  1:203794/125‑1 (MQ=255)                              tACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATc                                                                                                         <  1:47926/140‑1 (MQ=255)                               aCTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCt                                                                                                        >  1:630574/1‑140 (MQ=255)                               aCTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCt                                                                                                        >  2:1222126/1‑140 (MQ=255)                               aCTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCt                                                                                                        >  1:1157228/1‑140 (MQ=255)                                  ggAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAAt                                                                                                     <  1:1222126/140‑1 (MQ=255)                                    aaaGATGTCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTa                                                                                                   <  1:346799/140‑1 (MQ=255)                                         tGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATg                                                                                                              <  1:595038/124‑1 (MQ=255)                                         tGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATg                                                                                                              >  2:703029/1‑124 (MQ=255)                                         tGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATg                                                                                                              <  1:703029/124‑1 (MQ=255)                                         tGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATg                                                                                                              >  2:595038/1‑124 (MQ=255)                                         tGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACg                                                                                              >  1:449611/1‑140 (MQ=255)                                         tGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACg                                                                                              >  1:736409/1‑140 (MQ=255)                                         tGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACg                                                                                              >  2:414918/1‑140 (MQ=255)                                         tGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACg                                                                                              >  2:484353/1‑140 (MQ=255)                                          gCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGt                                                                                             <  2:630574/140‑1 (MQ=255)                                             gATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAg                                                                                          <  2:619879/140‑1 (MQ=255)                                              aTAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGc                                                                                         <  2:1051896/140‑1 (MQ=255)                                                  tCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCgtgg                                                                                     >  2:790987/1‑140 (MQ=255)                                                         tCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGt                                                                              >  2:358704/1‑140 (MQ=255)                                                           tGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCa                                                                            >  2:817662/1‑140 (MQ=255)                                                           tGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCa                                                                            >  1:291651/1‑140 (MQ=255)                                                             cccAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCAcc                                                                          >  1:251431/1‑140 (MQ=255)                                                                       gAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCg                                                                >  1:1144986/1‑140 (MQ=255)                                                                           cTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACa                                                            <  1:1010992/140‑1 (MQ=255)                                                                               aaaTGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCaa                                                        >  1:612138/1‑140 (MQ=255)                                                                                     tttGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCg                                                                <  2:1209705/126‑1 (MQ=255)                                                                                     tttGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCg                                                                >  1:1209705/1‑126 (MQ=255)                                                                                        gtgAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCt                                               >  2:1182627/1‑140 (MQ=255)                                                                                        gtgAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCt                                               >  1:493036/1‑140 (MQ=255)                                                                                              aaTTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGaa                                         <  2:251431/140‑1 (MQ=255)                                                                                                ttGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACg                                       <  1:817662/140‑1 (MQ=255)                                                                                                     gTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCtgtg                                  <  2:493036/140‑1 (MQ=255)                                                                                                     gTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCtgtg                                  <  2:736409/140‑1 (MQ=255)                                                                                                      ttAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGa                                 <  2:1144986/140‑1 (MQ=255)                                                                                                      ttAAAACCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGa                                 <  2:612138/140‑1 (MQ=255)                                                                                                          aaCCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCtgtg                                  >  2:131068/1‑135 (MQ=255)                                                                                                          aaCCAGGGATGCGCGTACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCtgtg                                  <  1:131068/135‑1 (MQ=255)                                                                                                                         tACTGGATATTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCAtt              <  1:1182627/140‑1 (MQ=255)                                                                                                                               atatTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTtgctg        <  1:414918/140‑1 (MQ=255)                                                                                                                                 atTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGt                     <  2:202897/125‑1 (MQ=255)                                                                                                                                 atTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGt                     >  1:202897/1‑125 (MQ=255)                                                                                                                                 atTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCa      >  2:860927/1‑140 (MQ=255)                                                                                                                                 atTGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCa      <  1:358704/140‑1 (MQ=255)                                                                                                                                  ttGGCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCaa     >  2:1098962/1‑140 (MQ=255)                                                                                                                                    ggCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGa   >  2:566202/1‑140 (MQ=255)                                                                                                                                    ggCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGa   <  2:1157228/140‑1 (MQ=255)                                                                                                                                     gCTGCAGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGAt  >  1:415772/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGCGAAGCTCAAAATGATTTGTGAAAAATTGCAGTTAAAACCAGGGATGCGCGTACTGGATATTGGCTGCGGCTGGGGCGGACTGGCACACTACATGGCATCTAATTATGACGTAAGCGTGGTGGGCGTCACCATTTCTGCCGAACAGCAAAAAATGGCTCAGGAACGCTGTGAAGGCCTGGATGTCACCATTTTGCTGCAAGAT  >  NC_000913/1741804‑1742075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑