Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,763,242	A→G	A256A (GCT→GCC)	sufB ←	Fe‑S cluster scaffold complex subunit SufB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,763,242	0	A	G	100.0%	174.0 / NA	48	A256A (GCT→GCC)	sufB	Fe‑S cluster scaffold complex subunit SufB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

TGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAG  >  NC_000913/1763106‑1763375                                                                                                                                         |                                                                                                                                      tGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCg                                                                                                                                    >  1:597857/1‑140 (MQ=255)  gACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCgg                                                                                                                                   >  1:998611/1‑140 (MQ=255)  gACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCgg                                                                                                                                   >  1:554871/1‑140 (MQ=255)    cGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGaa                                                                                                                                 <  1:607367/140‑1 (MQ=255)     gAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAAc                                                                                                                                >  1:110572/1‑140 (MQ=255)     gAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAAc                                                                                                                                <  2:32960/140‑1 (MQ=255)     gAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAAc                                                                                                                                <  2:554871/140‑1 (MQ=255)       aGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAg                                                                                                                              >  1:618368/1‑140 (MQ=255)                          gttgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGt                                                                                                           <  1:261251/140‑1 (MQ=255)                                   gCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGa                                                                                                              >  2:134235/1‑128 (MQ=255)                                   gCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGa                                                                                                              <  1:134235/128‑1 (MQ=255)                                   gCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCtt                                                                                                  >  1:529929/1‑140 (MQ=255)                                             cAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccac                                                                                        <  2:618368/140‑1 (MQ=255)                                                      tccGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGt                                                                               <  2:110572/139‑1 (MQ=255)                                                       ccGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTg                                                                              <  2:597857/140‑1 (MQ=255)                                                                    cACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGcc                                                                 >  1:537915/1‑140 (MQ=255)                                                                       cTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGCCGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCgg                                                              >  2:199731/1‑140 (MQ=255)                                                                         cGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGc                                                                  >  2:956585/1‑134 (MQ=255)                                                                         cGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGc                                                                  <  1:956585/134‑1 (MQ=255)                                                                           gCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGtttt                                                          >  1:235001/1‑140 (MQ=255)                                                                                    tGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGtt                                                 <  1:781810/140‑1 (MQ=255)                                                                                           tgaCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGa                                          >  2:886756/1‑140 (MQ=255)                                                                                            gaCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGaa                                         <  1:886756/140‑1 (MQ=255)                                                                                                        tGCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGaaa                             >  2:7670/1‑140 (MQ=255)                                                                                                         gCCGCGTGTAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAg                            <  2:537915/140‑1 (MQ=255)                                                                                                               tgtAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAg                            >  1:1131110/1‑134 (MQ=255)                                                                                                               tgtAACTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCAt                      >  2:317849/1‑140 (MQ=255)                                                                                                               tgtAACTGATAGCTGTCACGCACCGGGGCGGAACAGACTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAg                            <  2:1131110/134‑1 (MQ=255)                                                                                                                   aCTGATAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCggg                  <  1:7670/140‑1 (MQ=255)                                                                                                                       aTAGCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAgc              >  2:1189473/1‑140 (MQ=255)                                                                                                                          gCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  2:1198278/140‑1 (MQ=255)                                                                                                                          gCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  2:1123631/140‑1 (MQ=255)                                                                                                                          gCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  1:1189473/140‑1 (MQ=255)                                                                                                                          gCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  1:317849/140‑1 (MQ=255)                                                                                                                          gCTGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  2:617710/140‑1 (MQ=255)                                                                                                                            tGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACg         >  1:982848/1‑140 (MQ=255)                                                                                                                            tGTCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACg         >  2:932827/1‑140 (MQ=255)                                                                                                                              tCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           >  2:538126/1‑136 (MQ=255)                                                                                                                              tCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  2:655962/136‑1 (MQ=255)                                                                                                                              tCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           >  1:471957/1‑136 (MQ=255)                                                                                                                              tCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  1:538126/136‑1 (MQ=255)                                                                                                                              tCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           >  1:655962/1‑136 (MQ=255)                                                                                                                              tCACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  2:471957/136‑1 (MQ=255)                                                                                                                               cACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           >  1:51208/1‑135 (MQ=255)                                                                                                                               cACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  2:51208/135‑1 (MQ=255)                                                                                                                               cACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           >  1:179413/1‑135 (MQ=255)                                                                                                                               cACGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCa           <  2:179413/135‑1 (MQ=255)                                                                                                                                 cGCACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCttt    >  2:5480/1‑140 (MQ=255)                                                                                                                                   cACCGGGGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAg  >  2:960930/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAG  >  NC_000913/1763106‑1763375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑