Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,807,950	T→C	L52P (CTT→CCT)	yniA →	putative kinase YniA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,950	0	T	C	100.0%	279.8 / NA	79	L52P (CTT→CCT)	yniA	putative kinase YniA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/39);  total (40/39)

TTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTC  >  NC_000913/1807820‑1808084                                                                                                                                   |                                                                                                                                       ttGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGttt                                                                                                                               >  2:454766/1‑140 (MQ=255)  tGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGtttt                                                                                                                              >  1:797609/1‑140 (MQ=255)  tGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGtttt                                                                                                                              <  2:663957/140‑1 (MQ=255)    agcgagcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTAc                                                                                                                            >  1:481143/1‑140 (MQ=255)    agcgagcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTAAc                                                                                                                            >  1:953970/1‑140 (MQ=255)      cgagcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTAccg                                                                                                                          <  1:545479/140‑1 (MQ=255)        agcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTc                                                                                                                                      >  2:24401/1‑126 (MQ=255)        agcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTAccgcc                                                                                                                        >  1:234713/1‑140 (MQ=255)        agcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTAccgcc                                                                                                                        >  2:1048447/1‑140 (MQ=255)        agcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTAccgcc                                                                                                                        >  2:1182562/1‑140 (MQ=255)           aGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGaa                                                                                                                     <  1:835467/140‑1 (MQ=255)               aGGTGAAGGCAAAATCGAACTGCTTAATGACCTGCCGGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCACCCGGTTTTACCGCCGAAGCCg                                                                                                                 <  2:589876/140‑1 (MQ=255)                  tGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGCCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGAcc                                                                                                              <  1:256067/140‑1 (MQ=255)                   gAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCa                                                                                                             <  1:454766/140‑1 (MQ=255)                    aaGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCa                                                                                                             <  1:438341/139‑1 (MQ=255)                    aaGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCa                                                                                                             >  2:438341/1‑139 (MQ=255)                     aGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAAc                                                                                                           >  2:175172/1‑140 (MQ=255)                     aGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAAc                                                                                                           >  1:589876/1‑140 (MQ=255)                     aGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAAc                                                                                                           >  2:991702/1‑140 (MQ=255)                      ggCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACt                                                                                                          >  1:915288/1‑140 (MQ=255)                       gCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTg                                                                                                         >  2:704905/1‑140 (MQ=255)                       gCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTg                                                                                                         >  1:1196251/1‑140 (MQ=255)                           aaTCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGt                                                                                                     >  2:313973/1‑140 (MQ=255)                           aaTCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGt                                                                                                     >  2:771512/1‑140 (MQ=255)                                aaCTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTg                                                                                                >  1:157609/1‑140 (MQ=255)                                   tGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTcg                                                                                             <  2:797609/140‑1 (MQ=255)                                   tGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTcg                                                                                             <  1:175172/140‑1 (MQ=255)                                     cGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTcgcg                                                                                           <  2:967180/140‑1 (MQ=255)                                         aTGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCgtagt                                                                                       <  1:560053/140‑1 (MQ=255)                                               tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTcgcg                                                                                           <  2:774192/130‑1 (MQ=255)                                               tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTcgcg                                                                                           >  1:774192/1‑130 (MQ=255)                                               tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAAcc                                                                                 >  1:305675/1‑140 (MQ=255)                                               tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAAcc                                                                                 >  1:906674/1‑140 (MQ=255)                                                    ggCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAc                                                                            <  2:1124943/140‑1 (MQ=255)                                                    ggCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAc                                                                            <  2:1196251/140‑1 (MQ=255)                                                    ggCGGAGAACTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATTTGAGGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAc                                                                            <  2:280481/140‑1 (MQ=255)                                                     gCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAcc                                                                           <  1:158989/140‑1 (MQ=255)                                                           aaGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCt                                                                     >  2:99874/1‑140 (MQ=255)                                                                 aTGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCt                                                                     <  1:500078/134‑1 (MQ=255)                                                                 aTGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCt                                                                     >  2:500078/1‑134 (MQ=255)                                                                   gCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTg                                                             >  2:1199558/1‑140 (MQ=255)                                                                        aTGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAg                                                        >  1:433099/1‑140 (MQ=255)                                                                        aTGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAg                                                        >  1:400430/1‑140 (MQ=255)                                                                              tttGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGgcg                                                  <  2:940635/140‑1 (MQ=255)                                                                                 gcgcTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTg                                               <  1:99874/140‑1 (MQ=255)                                                                                   gcTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGAc                                             <  1:704905/140‑1 (MQ=255)                                                                                      aTGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGAcc                                            >  1:134713/1‑138 (MQ=255)                                                                                      aTGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGAcc                                            <  2:134713/138‑1 (MQ=255)                                                                                      aTGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGt                                          >  1:533268/1‑140 (MQ=255)                                                                                       tGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTg                                         >  2:655883/1‑140 (MQ=255)                                                                                           ggACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTa                                     <  2:157609/140‑1 (MQ=255)                                                                                           ggACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTa                                     <  2:915288/140‑1 (MQ=255)                                                                                           ggACATGATTTTTTCGTCAAATGTGATGAAAGGGAACGGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTa                                     <  2:234713/140‑1 (MQ=255)                                                                                            gACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTAc                                    >  2:287020/1‑140 (MQ=255)                                                                                               aTGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGt                                 >  2:125721/1‑140 (MQ=255)                                                                                                 gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGttt                               >  1:40595/1‑140 (MQ=255)                                                                                                 gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGttt                               >  2:742332/1‑140 (MQ=255)                                                                                                 gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGttt                               >  2:280617/1‑140 (MQ=255)                                                                                                 gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGttt                               >  1:34752/1‑140 (MQ=255)                                                                                                 gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGttt                               >  2:1200584/1‑140 (MQ=255)                                                                                                  aTTTTTTCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGtttt                              <  1:287020/140‑1 (MQ=255)                                                                                                     ttttCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTg                           <  1:1200584/140‑1 (MQ=255)                                                                                                     ttttCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTg                           <  1:1199558/140‑1 (MQ=255)                                                                                                        tCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTg                        <  2:324969/140‑1 (MQ=255)                                                                                                        tCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTg                        <  2:462250/140‑1 (MQ=255)                                                                                                        tCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTg                        <  2:176843/140‑1 (MQ=255)                                                                                                        tCGTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTg                        <  1:1048447/140‑1 (MQ=255)                                                                                                          gTCAAATGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGAt                      <  1:771512/140‑1 (MQ=255)                                                                                                             aaaTGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGa                   <  1:991702/140‑1 (MQ=255)                                                                                                             aaaTGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGa                   <  1:742332/140‑1 (MQ=255)                                                                                                             aaaTGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGa                   <  1:655883/140‑1 (MQ=255)                                                                                                              aaTGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGTTGACCGTGACTACAGTTTTCTGGTGATGGAt                  <  1:279478/140‑1 (MQ=255)                                                                                                              aaTGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGAt                  <  2:533268/140‑1 (MQ=255)                                                                                                              aaTGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGAt                  <  2:736443/140‑1 (MQ=255)                                                                                                               aTGTGATGAAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGAtt                 <  1:375440/140‑1 (MQ=255)                                                                                                                      gaAAGGGAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTccc          <  2:400430/140‑1 (MQ=255)                                                                                                                        aaGGGAGCTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCAc        >  1:1090544/1‑140 (MQ=255)                                                                                                                           ggAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAGCCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTc     <  1:280617/140‑1 (MQ=255)                                                                                                                            gAACTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCg    >  2:1039807/1‑140 (MQ=255)                                                                                                                              aCTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCAc        <  2:521637/134‑1 (MQ=255)                                                                                                                              aCTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCAc        >  1:521637/1‑134 (MQ=255)                                                                                                                              aCTGCCTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTc  >  2:408553/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                       TTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTC  >  NC_000913/1807820‑1808084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑