Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,877,189	T→C	L94P (CTC→CCC)	yeaO →	conserved protein YeaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,877,189	0	T	C	100.0%	181.5 / NA	50	L94P (CTC→CCC)	yeaO	conserved protein YeaO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/30);  total (20/30)

TGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTC  >  NC_000913/1877057‑1877322                                                                                                                                     |                                                                                                                                      tGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACt                                                                                                                                  >  1:589716/1‑138 (MQ=255) tGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACt                                                                                                                                  <  2:589716/138‑1 (MQ=255) tGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCa                                                                                                                                <  1:1202393/140‑1 (MQ=255) tGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCa                                                                                                                                >  1:199322/1‑140 (MQ=255)   cgcAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGc                                                                                                                              >  1:382027/1‑140 (MQ=255)        aGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCaaaaa                                                                                                                         >  1:67803/1‑140 (MQ=255)           cTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAaca                                                                                                                      <  1:1165859/140‑1 (MQ=255)           cTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAaca                                                                                                                      <  1:867335/140‑1 (MQ=255)           cTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAaca                                                                                                                      <  2:674812/140‑1 (MQ=255)              tCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAAcacca                                                                                                                   >  2:1040905/1‑140 (MQ=255)                aCGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACg                                                                                                                 <  1:1040905/140‑1 (MQ=255)                aCGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACg                                                                                                                 <  2:809372/140‑1 (MQ=255)                  ggCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCa                                                                                                               <  2:1058144/140‑1 (MQ=255)                     gAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGaa                                                                                                            <  1:853258/140‑1 (MQ=255)                      aaGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCGGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAaca                                                                                                                      >  2:567883/1‑129 (MQ=255)                      aaGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAaca                                                                                                                      <  1:567883/129‑1 (MQ=255)                                   tGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGc                                                                                              >  1:320541/1‑140 (MQ=255)                                          tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGa                                                                                       <  1:526741/140‑1 (MQ=255)                                                   cAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACg                                                                              >  2:298578/1‑140 (MQ=255)                                                   cAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACg                                                                              >  2:285883/1‑140 (MQ=255)                                                    aaTATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGt                                                                             <  2:67803/140‑1 (MQ=255)                                                           tGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtgt                                                                      >  2:936712/1‑140 (MQ=255)                                                           tGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtgt                                                                      <  1:500727/140‑1 (MQ=255)                                                              aGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAt                                                                   <  2:320541/140‑1 (MQ=255)                                                              aGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAt                                                                   <  2:199322/140‑1 (MQ=255)                                                                aaCTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtgt                                                                      >  1:501866/1‑135 (MQ=255)                                                                aaCTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtgt                                                                      >  1:236202/1‑135 (MQ=255)                                                                aaCTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtgt                                                                      <  2:501866/135‑1 (MQ=255)                                                                aaCTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTtgt                                                                      <  2:236202/135‑1 (MQ=255)                                                                aaCTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAttt                                                                 >  1:8827/1‑140 (MQ=255)                                                                  cTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTa                                                               >  1:1093040/1‑140 (MQ=255)                                                                  cTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTa                                                               >  1:726269/1‑140 (MQ=255)                                                                  cTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTa                                                               >  1:1134908/1‑140 (MQ=255)                                                                  cTGGCGCAACACGAGCAAGAAAGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTa                                                               >  2:454493/1‑140 (MQ=255)                                                                     gcgcAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTa                                                            >  2:535659/1‑140 (MQ=255)                                                                               aGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCcgg                                                  <  1:630465/140‑1 (MQ=255)                                                                                   agaagGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCcg                                                   <  2:173526/135‑1 (MQ=255)                                                                                   agaagGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCcg                                                   >  1:173526/1‑135 (MQ=255)                                                                                   agaaagaaAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGt                                              <  1:454493/140‑1 (MQ=255)                                                                                             cGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCAc                                    <  2:726269/140‑1 (MQ=255)                                                                                             cGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTCGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCAc                                    <  2:1093040/140‑1 (MQ=255)                                                                                              ggCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCAcc                                   <  1:936712/140‑1 (MQ=255)                                                                                               gCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCa                                  >  1:1222061/1‑140 (MQ=255)                                                                                               gCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCa                                  <  2:1222061/140‑1 (MQ=255)                                                                                                         aTCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGt                        <  1:285883/140‑1 (MQ=255)                                                                                                                aaaaaCAGCCGCTGCCCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCAcgccg                 <  1:280151/140‑1 (MQ=255)                                                                                                                 aaaaCAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCAcgccgc                <  2:1134908/140‑1 (MQ=255)                                                                                                                       gCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAAt          <  2:112524/140‑1 (MQ=255)                                                                                                                         cGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGc        <  1:397805/140‑1 (MQ=255)                                                                                                                               cccTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATtc  <  1:862046/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      TGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTC  >  NC_000913/1877057‑1877322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑