Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,882,687	G→A	G259D (GGC→GAC)	dmlA →	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,882,687	0	G	A	100.0%	137.9 / NA	42	G259D (GGC→GAC)	dmlA	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/21);  total (21/21)

GGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGC  >  NC_000913/1882553‑1882819                                                                                                                                       |                                                                                                                                     ggAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCa                                                                                                                                 >  1:689245/1‑140 (MQ=255)                  agcagcATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATcc                                                                                                                >  1:979357/1‑140 (MQ=255)                   gcagcaTATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCg                                                                                                               <  2:689245/140‑1 (MQ=255)                     agcaTATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGcc                                                                                                             <  2:848077/140‑1 (MQ=255)                     agcaTATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGcc                                                                                                             <  2:601991/140‑1 (MQ=255)                         tatTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAAcc                                                                                                         >  1:473962/1‑140 (MQ=255)                            tGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGa                                                                                                      >  2:955168/1‑140 (MQ=255)                                  tctcTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCgg                                                                                                >  1:664356/1‑140 (MQ=255)                                   ctctGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGa                                                                                               <  2:979357/140‑1 (MQ=255)                                    tctGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGTGGGTGGCGTACAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGaa                                                                                              >  2:1003805/1‑140 (MQ=255)                                    tctGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGaa                                                                                              >  1:102899/1‑140 (MQ=255)                                    tctGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGaa                                                                                              >  2:623807/1‑140 (MQ=255)                                     ctGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAA‑TTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACg                                                                                            >  1:421048/1‑140 (MQ=255)                                      tGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACg                                                                                            <  1:303908/140‑1 (MQ=255)                                      tGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACg                                                                                            >  2:562695/1‑140 (MQ=255)                                      tGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACg                                                                                            >  2:822695/1‑140 (MQ=255)                                      tGCGCGCGTTTTGTGATGCAGCC‑GAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGc                                                                                           >  2:663815/1‑140 (MQ=255)                                        cgcgcgTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACg                                                                                            <  2:398815/138‑1 (MQ=255)                                        cgcgcgTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACg                                                                                            >  1:398815/1‑138 (MQ=255)                                                   tgATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCg                                                                               <  2:982659/140‑1 (MQ=255)                                                        cAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCg                                                                               <  1:1057330/135‑1 (MQ=255)                                                        cAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCg                                                                               >  2:1057330/1‑135 (MQ=255)                                                        cAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCtt                                                                          <  1:1020468/140‑1 (MQ=255)                                                        cAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCtt                                                                          <  1:562695/140‑1 (MQ=255)                                                         aGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTc                                                                         >  1:811895/1‑140 (MQ=255)                                                          gCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCg                                                                        >  1:677709/1‑140 (MQ=255)                                                          agccgAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCg                                                                        <  1:663815/136‑1 (MQ=255)                                                           ccGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCg                                                                               >  1:850114/1‑132 (MQ=255)                                                           ccGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCg                                                                               <  2:850114/132‑1 (MQ=255)                                                            cGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAg                                                                      <  2:677709/140‑1 (MQ=255)                                                             ggAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGc                                                                     <  1:619834/140‑1 (MQ=255)                                                                    ttCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCa                                                              <  1:822695/140‑1 (MQ=255)                                                                    ttCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCa                                                              <  1:623807/140‑1 (MQ=255)                                                                               ggtggCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCgcgc                                                   <  1:17743/140‑1 (MQ=255)                                                                                gtggCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGcc                                                  <  1:970564/140‑1 (MQ=255)                                                                                   gCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGa                                               >  2:660276/1‑140 (MQ=255)                                                                                    cGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGat                                              <  2:102899/140‑1 (MQ=255)                                                                                                       tatCCTTTCCGATCTTGGCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTg                           >  2:341889/1‑140 (MQ=255)                                                                                                                       ggCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCAc           >  1:765712/1‑140 (MQ=255)                                                                                                                       ggCCCGGCCTGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCAc           >  2:293952/1‑140 (MQ=255)                                                                                                                                tGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGc  <  1:955168/140‑1 (MQ=255)                                                                                                                                tGCACCGACACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGc  <  2:421048/140‑1 (MQ=255)                                                                                                                                tGCACCGACACCATTGGCATTGCCCAATCCGCCAACCTAAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTAGCGCCACGATTTGGGc  <  1:430684/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     GGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGATTCGATGTGGTGGTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGCCAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATATTGCTAACCCTATCGCCACGATTTGGGC  >  NC_000913/1882553‑1882819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑