Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,006,753	C→T	L200L (CTG→TTG)	amyA →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,006,753	0	C	T	100.0%	242.8 / NA	68	L200L (CTG→TTG)	amyA	alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/32);  total (36/32)

TCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATCTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACATATTCCAGCC  >  NC_000913/2006616‑2006881                                                                                                                                          |                                                                                                                                   tCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTg                                                                                                                                  >  2:1069176/1‑140 (MQ=255)  cTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGa                                                                                                                                 <  1:548966/140‑1 (MQ=255)    gggATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATg                                                                                                                               <  1:291197/140‑1 (MQ=255)     ggATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATgg                                                                                                                              >  1:38450/1‑140 (MQ=255)     ggATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATgg                                                                                                                              >  2:277719/1‑140 (MQ=255)         ttCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCga                                                                                                                          >  2:142834/1‑140 (MQ=255)         ttCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCga                                                                                                                          <  2:38450/140‑1 (MQ=255)         ttCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGGAATTTCGATTATTTGATGGGCga                                                                                                                          >  2:858128/1‑140 (MQ=255)           cAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATg                                                                                                                               >  1:446021/1‑133 (MQ=255)           cAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATg                                                                                                                               <  2:446021/133‑1 (MQ=255)                 ttttAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCg                                                                                                                  >  1:759836/1‑140 (MQ=255)                   ttAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGAt                                                                                                                >  2:942097/1‑140 (MQ=255)                    tAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGAtt                                                                                                               >  1:472677/1‑140 (MQ=255)                     aGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAGTATCGAttt                                                                                                              >  1:985378/1‑140 (MQ=255)                        ggTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCg                                                                                                           <  2:472677/140‑1 (MQ=255)                         gTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGc                                                                                                          >  1:582692/1‑140 (MQ=255)                          tATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCa                                                                                                         >  2:485091/1‑140 (MQ=255)                              gACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCa                                                                                                     <  1:942097/140‑1 (MQ=255)                                   tatCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCg                                                                                                >  1:475970/1‑140 (MQ=255)                                    atCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGt                                                                                               >  1:1217959/1‑140 (MQ=255)                                      cGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTACTTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGa                                                                                             >  1:866191/1‑140 (MQ=255)                                          aaCCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGa                                                                                         <  1:21462/140‑1 (MQ=255)                                          aaCCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGa                                                                                         >  2:1051804/1‑140 (MQ=255)                                          aaCCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGa                                                                                         >  1:1108829/1‑140 (MQ=255)                                            cccTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑ag                                                                                     <  1:1051804/140‑1 (MQ=255)                                            cccTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑ag                                                                                     <  1:1069176/140‑1 (MQ=255)                                            cccTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑ag                                                                                     <  2:472044/140‑1 (MQ=255)                                            cccTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑ag                                                                                     <  2:582692/140‑1 (MQ=255)                                            cccTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑ag                                                                                     <  2:866191/140‑1 (MQ=255)                                                gACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGAt                                                                                 <  1:363424/140‑1 (MQ=255)                                                      gATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATa                                                                           >  2:561838/1‑140 (MQ=255)                                                                     aTTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGAGGTGCGGGAATATCGATTTGCGCGGTCATGCCGTGACGGG‑‑AGAGATTAAATACTGGGCGCGATGGGt                                                            <  2:462131/140‑1 (MQ=255)                                                                         ttAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATg                                                        >  1:147206/1‑140 (MQ=255)                                                                          tAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGAAGAGAGATTAAATACTGGGCGCGCTGGGTGAt                                                         <  2:475970/140‑1 (MQ=255)                                                                             cGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAAc                                                    <  1:1138267/140‑1 (MQ=255)                                                                                   cacCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACg                                               <  1:1146043/139‑1 (MQ=255)                                                                                   cacCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACg                                               >  2:1146043/1‑139 (MQ=255)                                                                                        gCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGc                                         >  2:449999/1‑140 (MQ=255)                                                                                        gCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGc                                         >  1:992876/1‑140 (MQ=255)                                                                                        gCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACACAATGc                                         >  2:37839/1‑140 (MQ=255)                                                                                           aaGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGAc                                      >  1:790207/1‑140 (MQ=255)                                                                                             ggCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACgg                                    >  2:415394/1‑140 (MQ=255)                                                                                             ggCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACgg                                    >  2:524556/1‑140 (MQ=255)                                                                                                tGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGttt                                 <  2:147206/140‑1 (MQ=255)                                                                                                  gAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTc                               >  2:229044/1‑140 (MQ=255)                                                                                                        tCAGGTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTg                         >  1:1336/1‑140 (MQ=255)                                                                                                           ggTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATg                      <  1:229044/140‑1 (MQ=255)                                                                                                           ggTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATg                      <  1:524556/140‑1 (MQ=255)                                                                                                            gTTGATGATGAATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGc                     >  1:825827/1‑140 (MQ=255)                                                                                                               gatgatgaATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTAGTCTTGATGCGGt                  <  1:142834/140‑1 (MQ=255)                                                                                                                atgatgaATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTc                 <  2:992876/140‑1 (MQ=255)                                                                                                                atgatgaATTAGGTAATTTCGATTATTTGATGGGCGAGAATACCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTc                 <  2:1139749/140‑1 (MQ=255)                                                                                                                  gatgaATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTc                 <  2:1145567/138‑1 (MQ=255)                                                                                                                  gatgaATTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTc                 >  1:1145567/1‑138 (MQ=255)                                                                                                                    tgaATTAGGTAATTTCGATTATTTGATGGGCGAGAGTATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAAc             <  2:985378/140‑1 (MQ=255)                                                                                                                      aaTTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACat           >  2:983160/1‑140 (MQ=255)                                                                                                                      aaTTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACat           >  1:258687/1‑140 (MQ=255)                                                                                                                       aTTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATg                      <  2:967501/128‑1 (MQ=255)                                                                                                                       aTTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATg                      >  1:967501/1‑128 (MQ=255)                                                                                                                       aTTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTc                 >  2:775357/1‑133 (MQ=255)                                                                                                                       aTTAGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTc                 <  1:775357/133‑1 (MQ=255)                                                                                                                          aGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCaaa              >  2:258297/1‑133 (MQ=255)                                                                                                                          aGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCaaa              <  1:258297/133‑1 (MQ=255)                                                                                                                          aGGTAATTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACATATTc       <  2:825827/140‑1 (MQ=255)                                                                                                                               aTTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACATATTCCAGcc  <  1:277719/140‑1 (MQ=255)                                                                                                                               aTTTCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACATATTCCAGcc  <  1:415394/140‑1 (MQ=255)                                                                                                                                tttCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGt                  >  2:356011/1‑123 (MQ=255)                                                                                                                                tttCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGt                  <  1:356011/123‑1 (MQ=255)                                                                                                                                  tCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACATATTCCGGcc  >  2:748904/1‑137 (MQ=255)                                                                                                                                  tCGATTATTTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACATATTCCGGcc  <  1:748904/137‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   TCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACCCTGACGAAGATGGCATTTTTAAAATTGTTAACGACTACACCGGCGAAGGCTGGAACGATCAGGTTGATGATGAATTAGGTAATTTCGATTATCTGATGGGCGAGAATATCGATTTTCGCAATCATGCCGTGACGGA‑‑AGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGTTTTCGTCTTGATGCGGTCAAACATATTCCAGCC  >  NC_000913/2006616‑2006881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑