Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,070,892	T→C	pseudogene (107/426 nt)	yeeP →	CP4‑44 prophage; putative GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,070,892	0	T	C	100.0%	200.3 / NA	55	pseudogene (107/426 nt)	yeeP	CP4‑44 prophage; putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/30);  total (25/30)

CTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGTATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGAC  >  NC_000913/2070756‑2071023                                                                                                                                         |                                                                                                                                    cTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCAtt                                                                                                                                  <  2:81677/140‑1 (MQ=255)     aGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGt                                                                                                                              <  1:1168611/140‑1 (MQ=255)      gTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTc                                                                                                                             >  2:122650/1‑140 (MQ=255)        gCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTg                                                                                                                           >  1:694125/1‑140 (MQ=255)          ggACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGtt                                                                                                                         <  2:348960/140‑1 (MQ=255)             cATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCg                                                                                                                      <  1:1078113/140‑1 (MQ=255)             cATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCg                                                                                                                      <  1:598681/140‑1 (MQ=255)             cATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCg                                                                                                                      <  1:415325/140‑1 (MQ=255)               tCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGc                                                                                                                    <  2:1151308/140‑1 (MQ=255)                 agcagGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGccc                                                                                                                  <  1:202051/140‑1 (MQ=255)                   cagGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGt                                                                                                                <  1:285875/140‑1 (MQ=255)                        gCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATc                                                                                                           <  1:233955/140‑1 (MQ=255)                            tttGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGt                                                                                                       <  2:1038094/140‑1 (MQ=255)                                      cGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTa                                                                                             >  2:347720/1‑140 (MQ=255)                                              gACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCg                                                                                     <  1:169755/140‑1 (MQ=255)                                                      ggAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCt                                                                             <  2:694125/140‑1 (MQ=255)                                                          cccTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCt                                                                             >  2:1184163/1‑136 (MQ=255)                                                          cccTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCt                                                                             <  1:1184163/136‑1 (MQ=255)                                                           ccTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGaa                                                                        <  1:122650/140‑1 (MQ=255)                                                           ccTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGaa                                                                        <  1:347720/140‑1 (MQ=255)                                                               ccATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTgg                                                                    <  2:360494/140‑1 (MQ=255)                                                               ccATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTgg                                                                    <  2:631977/140‑1 (MQ=255)                                                                    aaTGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACg                                                               <  2:88345/140‑1 (MQ=255)                                                                         gATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGt                                                          >  2:929725/1‑140 (MQ=255)                                                                         gATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGt                                                          >  2:916534/1‑140 (MQ=255)                                                                         gATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGt                                                          >  2:827487/1‑140 (MQ=255)                                                                          atatGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTc                                                         >  1:889024/1‑140 (MQ=255)                                                                              ggCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGt                                                      >  2:903554/1‑139 (MQ=255)                                                                              ggCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGt                                                      <  1:903554/139‑1 (MQ=255)                                                                              ggCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTg                                                     >  2:153091/1‑140 (MQ=255)                                                                                ccGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTgcg                                                   >  1:910032/1‑140 (MQ=255)                                                                                ccGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTgcg                                                   >  1:754775/1‑140 (MQ=255)                                                                                      ttCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATg                                             <  1:156260/140‑1 (MQ=255)                                                                                          gCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAg                                         <  1:674823/140‑1 (MQ=255)                                                                                             ttCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGTTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAg                                         <  2:1081611/137‑1 (MQ=255)                                                                                             ttCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAg                                         >  1:1081611/1‑137 (MQ=255)                                                                                              tctcCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCt                                     >  1:1124369/1‑140 (MQ=255)                                                                                              tctcCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCt                                     >  2:76105/1‑140 (MQ=255)                                                                                                        aggcacagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCAt                           >  2:735307/1‑140 (MQ=255)                                                                                                          gcacagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgc                         >  2:1141531/1‑140 (MQ=255)                                                                                                          gcacagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgc                         >  2:169194/1‑140 (MQ=255)                                                                                                          gcacagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgc                         >  2:1035137/1‑140 (MQ=255)                                                                                                          gcacagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgc                         >  1:135417/1‑140 (MQ=255)                                                                                                          gcacagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgc                         >  1:148945/1‑140 (MQ=255)                                                                                                          gcacagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgc                         >  2:549165/1‑140 (MQ=255)                                                                                                          gcacagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgc                         >  1:396418/1‑140 (MQ=255)                                                                                                             cagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgccgc                      <  2:148945/140‑1 (MQ=255)                                                                                                             cagAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATgccgc                      <  1:827487/140‑1 (MQ=255)                                                                                                               gAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCa                    <  1:916534/140‑1 (MQ=255)                                                                                                               gAATATTCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCa                    <  1:929725/140‑1 (MQ=255)                                                                                                                    ttCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTccc               >  1:24126/1‑140 (MQ=255)                                                                                                                    ttCGCGAAAAAACGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTccc               >  2:1079404/1‑140 (MQ=255)                                                                                                                           aaaaaCGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCtgatga        <  1:76105/140‑1 (MQ=255)                                                                                                                             aaaCGGAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGAcc      <  2:889024/140‑1 (MQ=255)                                                                                                                                 ggAGGCGGCATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGAc  <  1:1141531/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATATTCGCGAAAAAACGGAGGCGGTATTCCGTCTGTTCCGGCCCGTACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGAC  >  NC_000913/2070756‑2071023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑