Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,065	T→C	Q144R (CAG→CGG)	fruB ←	fructose‑specific PTS multiphosphoryl transfer protein FruB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,065	0	T	C	100.0%	211.9 / NA	58	Q144R (CAG→CGG)	fruB	fructose‑specific PTS multiphosphoryl transfer protein FruB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/32);  total (26/32)

TTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGG  >  NC_000913/2262932‑2263194                                                                                                                                      |                                                                                                                                  tttGGTGACGAAAGGGGCGTCAACTCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCtttt                                                                                                                             <  1:571908/140‑1 (MQ=255)   tGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCg                                                                                                                           <  1:446025/140‑1 (MQ=255)   gggTGACGAAGGTGGCGCCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCg                                                                                                                           <  2:859115/139‑1 (MQ=255)        agaaaaGTGGCGCCAACAGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCAt                                                                                                                      <  2:47100/137‑1 (MQ=255)                 gCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCg                                                                                                                           >  1:674781/1‑126 (MQ=255)                 gCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCg                                                                                                                           <  2:674781/126‑1 (MQ=255)                           cccccGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGttct                                                                                                   <  1:358063/140‑1 (MQ=255)                               cGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCGCTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTctg                                                                                               <  2:1194106/140‑1 (MQ=255)                                gCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTctgc                                                                                              >  1:901905/1‑140 (MQ=255)                                        cAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGttgttg                                                                                      <  2:338434/140‑1 (MQ=255)                                              cgcAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGAc                                                                                 >  2:451230/1‑139 (MQ=255)                                              cgcAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGAc                                                                                 <  1:451230/139‑1 (MQ=255)                                              cgcAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACt                                                                                >  2:1112760/1‑140 (MQ=255)                                              cgcAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACt                                                                                >  1:422247/1‑140 (MQ=255)                                              cgcAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACt                                                                                >  2:1221880/1‑140 (MQ=255)                                              cgcAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACt                                                                                >  2:302346/1‑140 (MQ=255)                                               gcAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACtt                                                                               >  2:409671/1‑140 (MQ=255)                                                cAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTc                                                                              >  2:732456/1‑140 (MQ=255)                                                  gCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAg                                                                            <  1:302346/140‑1 (MQ=255)                                                  gCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAg                                                                            <  2:422247/140‑1 (MQ=255)                                                  gCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAg                                                                            <  2:4105/140‑1 (MQ=255)                                                     ttCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAgttg                                                                         >  2:636356/1‑140 (MQ=255)                                                       cAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAgttgtt                                                                       <  1:1221880/140‑1 (MQ=255)                                                       cAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAgttgtt                                                                       <  1:366250/140‑1 (MQ=255)                                                         gcgcCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCa                                                                     <  2:443655/140‑1 (MQ=255)                                                         gcgcCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCa                                                                     >  1:101875/1‑140 (MQ=255)                                                           gcCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGc                                                                   <  1:273847/140‑1 (MQ=255)                                                               gAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACg                                                               >  1:330367/1‑140 (MQ=255)                                                               gAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACg                                                               >  1:1065686/1‑140 (MQ=255)                                                                aaGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACgg                                                              <  2:101875/140‑1 (MQ=255)                                                                     tCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGAAACGGAatca                                                         <  1:732456/140‑1 (MQ=255)                                                                     tCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCAGACTTCAGTTGTTCAGCAACGGAatca                                                         <  2:473476/140‑1 (MQ=255)                                                                       agcagATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAatcatc                                                       >  2:120858/1‑140 (MQ=255)                                                                          agATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCt                                                    <  1:1112760/140‑1 (MQ=255)                                                                            aTCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCt                                                    <  1:583652/138‑1 (MQ=255)                                                                            aTCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCt                                                    >  2:583652/1‑138 (MQ=255)                                                                             tCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAg                                                 >  1:1196302/1‑140 (MQ=255)                                                                              cGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGt                                                >  1:537790/1‑140 (MQ=255)                                                                                     gacgaTATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTggg                                         <  2:901905/140‑1 (MQ=255)                                                                                     gacgaTATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGCAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTggg                                         <  2:1065686/140‑1 (MQ=255)                                                                                      acgaTATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGt                                        >  2:462622/1‑140 (MQ=255)                                                                                                 tgtCAGCATTTCGTTGTCGAGCTTCTTCTGCTCACTCCGCTTTTCGCCCATCAGCAATGCGCGTCGCTCTTCTGGTGTTGTTGCTTACTTCAGTTGTTCAGCAACGTAATCATCGCTCAGTACGTAAGTCAGCTgactta                             >  2:713225/1‑135 (MQ=255)                                                                                                 tgtCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTa                             >  1:461774/1‑140 (MQ=255)                                                                                                 tgtCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTa                             >  1:628653/1‑140 (MQ=255)                                                                                                 tgtCAGCATTTCGTTGACGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTa                             >  2:918634/1‑140 (MQ=255)                                                                                                  gtCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAg                            >  1:822269/1‑140 (MQ=255)                                                                                                  gtCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAg                            <  1:636356/140‑1 (MQ=255)                                                                                                  gtCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAg                            <  2:628653/140‑1 (MQ=255)                                                                                                   tCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGc                           <  2:822269/140‑1 (MQ=255)                                                                                                     aGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAg                         >  2:1221585/1‑140 (MQ=255)                                                                                                      gCATTTCGTTGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAgg                        >  2:634604/1‑140 (MQ=255)                                                                                                              ttGTCGAGCTTCAGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATg                <  1:403212/140‑1 (MQ=255)                                                                                                                          aGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCt    <  1:634604/140‑1 (MQ=255)                                                                                                                          aGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCt    <  2:1196302/140‑1 (MQ=255)                                                                                                                          aGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCt    <  1:1221585/140‑1 (MQ=255)                                                                                                                          aGCTGCTCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCt    <  2:330367/140‑1 (MQ=255)                                                                                                                           gctgctCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTg   >  2:1045443/1‑140 (MQ=255)                                                                                                                            ctgctCACTCCGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTgg  <  1:120858/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  TTTGGTGACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGG  >  NC_000913/2262932‑2263194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑