Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,648	T→C	L50L (TTA→TTG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,648	0	T	C	100.0%	201.7 / NA	55	L50L (TTA→TTG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

AGCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGA  >  NC_000913/2329513‑2329781                                                                                                                                        |                                                                                                                                      aGCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATg                                                                                                                                   >  2:838371/1‑140 (MQ=255)  gCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGc                                                                                                                                  <  2:59759/140‑1 (MQ=255)   ccTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCt                                                                                                                                 <  1:996895/140‑1 (MQ=255)   ccTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCt                                                                                                                                 >  1:39771/1‑140 (MQ=255)    cTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTg                                                                                                                                <  2:948980/140‑1 (MQ=255)    cTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTg                                                                                                                                >  1:562976/1‑140 (MQ=255)      gACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTgag                                                                                                                              >  2:116560/1‑140 (MQ=255)        caacaaTAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTTAGGCAATGCTGAGAc                                                                                                                            >  2:38308/1‑140 (MQ=255)         aacaaTAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACt                                                                                                                           <  1:838371/140‑1 (MQ=255)             aTAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTgg                                                                                                                       >  1:714105/1‑140 (MQ=255)                 aCCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCAc                                                                                                                   >  2:919711/1‑140 (MQ=255)                   ctctTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGc                                                                                                                 <  2:714105/140‑1 (MQ=255)                      ttCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCtg                                                                                                              >  1:477086/1‑140 (MQ=255)                            ggAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTTAGGCAATGCTGAGACTTTGGTCACGCCTGATgacg                                                                                                        <  1:38308/140‑1 (MQ=255)                            ggAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATgacg                                                                                                        <  1:769241/140‑1 (MQ=255)                             gAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCtgat                                                                                                            <  1:810079/135‑1 (MQ=255)                             gAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCtgat                                                                                                            >  2:810079/1‑135 (MQ=255)                              aGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATgacgac                                                                                                      <  2:195527/140‑1 (MQ=255)                                tttCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAg                                                                                                    >  2:321750/1‑140 (MQ=255)                                 ttCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGc                                                                                                   <  2:477086/140‑1 (MQ=255)                                        acacTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACa                                                                                            <  1:1213223/140‑1 (MQ=255)                                          acTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGt                                                                                          >  2:60181/1‑140 (MQ=255)                                            tGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGAt                                                                                               <  2:502755/133‑1 (MQ=255)                                            tGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGAt                                                                                               >  1:502755/1‑133 (MQ=255)                                                  ggCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGtg                                                                                  <  1:293864/140‑1 (MQ=255)                                                  ggCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTAAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGtg                                                                                  <  2:1009231/140‑1 (MQ=255)                                                        tCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTgcgc                                                                            >  1:189369/1‑140 (MQ=255)                                                        tCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTgcgc                                                                            >  1:149189/1‑140 (MQ=255)                                                               gATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCgcgc                                                                     <  2:39771/140‑1 (MQ=255)                                                                aTGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgca                                                                    <  1:116560/140‑1 (MQ=255)                                                                     tATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCAcc                                                               <  1:321750/140‑1 (MQ=255)                                                                      aTCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCa                                                              >  2:1003137/1‑140 (MQ=255)                                                                            cagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTa                                                        <  1:824667/140‑1 (MQ=255)                                                                               ccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACg                                                     >  1:39474/1‑140 (MQ=255)                                                                                 agGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGc                                                   <  2:583166/140‑1 (MQ=255)                                                                                  ggCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCg                                                  >  2:176273/1‑140 (MQ=255)                                                                                   gCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGt                                                 >  2:700811/1‑140 (MQ=255)                                                                                    cGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGcatc                                                <  2:149189/140‑4 (MQ=255)                                                                                           aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCg                                         <  1:60181/140‑1 (MQ=255)                                                                                               aGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAg                                     <  1:1003137/140‑1 (MQ=255)                                                                                                   tCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCg                                         <  1:1122813/132‑1 (MQ=255)                                                                                                   tCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCg                                         >  2:1122813/1‑132 (MQ=255)                                                                                                     cacaAACTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg                               >  1:79113/1‑140 (MQ=255)                                                                                                         aaCTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCaga                           <  1:176273/140‑1 (MQ=255)                                                                                                          aCTCCCTAACGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCagag                          >  1:394612/1‑140 (MQ=255)                                                                                                                 aaCGGCGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCaa                   >  1:698837/1‑140 (MQ=255)                                                                                                                      cGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCagag                          <  2:184280/128‑1 (MQ=255)                                                                                                                      cGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCagag                          >  1:184280/1‑128 (MQ=255)                                                                                                                      cGTGGTGAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCa              >  1:513081/1‑140 (MQ=255)                                                                                                                        tggtgAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAAt            >  2:325873/1‑140 (MQ=255)                                                                                                                        tggtgAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAAt            >  2:790399/1‑140 (MQ=255)                                                                                                                        tggtgAGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAAt            >  1:1085320/1‑140 (MQ=255)                                                                                                                             aGCGAGTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCa       >  1:921016/1‑140 (MQ=255)                                                                                                                                  gTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGa  >  1:602960/1‑140 (MQ=255)                                                                                                                                  gTCAGGCAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGa  >  2:777843/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      AGCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACGCCAATAGCCAGACGA  >  NC_000913/2329513‑2329781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑