Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,510,259	G→A	L210L (CTG→CTA)	yfeO →	putative transport protein YfeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,510,259	0	G	A	100.0%	149.2 / NA	45	L210L (CTG→CTA)	yfeO	putative transport protein YfeO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/27);  total (18/27)

GGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGA  >  NC_000913/2510124‑2510380                                                                                                                                        |                                                                                                                          ggcggcgTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCa                                                                                                                       <  1:762674/140‑1 (MQ=255)      cgTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCt                                                                                                                  <  2:96785/140‑1 (MQ=255)      cgTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCt                                                                                                                  <  1:705287/140‑1 (MQ=255)             atTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACg                                                                                                           <  2:642769/140‑1 (MQ=255)               tttCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGa                                                                                                         <  2:1106705/140‑1 (MQ=255)               tttCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGa                                                                                                         <  2:652718/140‑1 (MQ=255)                  cGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAg                                                                                                      >  2:206206/1‑140 (MQ=255)                     aaaCCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACgg                                                                                                          <  2:191262/133‑1 (MQ=255)                     aaaCCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACgg                                                                                                          >  1:191262/1‑133 (MQ=255)                        ccTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGaa                                                                                                <  1:1165929/140‑1 (MQ=255)                          ttAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAAt                                                                                              <  1:979399/140‑1 (MQ=255)                               tGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCg                                                                                         <  1:1201604/140‑1 (MQ=255)                                   agtagtGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGatat                                                                                     >  1:448742/1‑140 (MQ=255)                                   agtagtGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGatat                                                                                     >  2:1031233/1‑140 (MQ=255)                                         gAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAg                                                                               <  2:835778/140‑1 (MQ=255)                                            gTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCgg                                                                            >  1:1067869/1‑140 (MQ=255)                                                  cTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAAt                                                                      >  1:1208680/1‑140 (MQ=255)                                                             tctcTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGa                                                           >  2:400223/1‑140 (MQ=255)                                                             tctcTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGa                                                           >  1:459209/1‑140 (MQ=255)                                                                 tttGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGc                                                       >  2:1044451/1‑140 (MQ=255)                                                                   tGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCa                                                     <  1:1044451/140‑1 (MQ=255)                                                                      gcCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCAt                                                    <  1:811546/138‑1 (MQ=255)                                                                      gcCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCAt                                                    >  2:811546/1‑138 (MQ=255)                                                                       ccGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc                                                 >  1:455597/1‑140 (MQ=255)                                                                       ccGTTAATGGCGGCAGCAGCTGGGGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgc                                                 >  2:1132578/1‑140 (MQ=255)                                                                          ttAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCgcagc                                              >  2:746658/1‑140 (MQ=255)                                                                                   gcagcaGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGt                                     <  1:206206/140‑1 (MQ=255)                                                                                     agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCg                                   <  1:746658/140‑1 (MQ=255)                                                                                     agcagcTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCg                                   <  2:459209/140‑1 (MQ=255)                                                                                          cTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTa                              <  1:400223/140‑1 (MQ=255)                                                                                          cTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTa                              <  2:812950/140‑1 (MQ=255)                                                                                          cTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTa                              <  1:131263/140‑1 (MQ=255)                                                                                               gCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTg                         <  1:1031233/140‑1 (MQ=255)                                                                                                        acCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTa                              <  2:390953/126‑1 (MQ=255)                                                                                                        acCGGATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTa                              >  1:390953/1‑126 (MQ=255)                                                                                                           ggATTATTTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGtt             <  2:1208680/140‑1 (MQ=255)                                                                                                             attattTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGc           >  2:189139/1‑140 (MQ=255)                                                                                                             attattTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGc           <  2:571852/140‑1 (MQ=255)                                                                                                             attattTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGc           >  2:1063632/1‑140 (MQ=255)                                                                                                               tattTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCAc         >  2:153322/1‑140 (MQ=255)                                                                                                                attTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCAcg        <  2:455597/140‑1 (MQ=255)                                                                                                                attTTTCCATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCAcg        <  1:153322/140‑1 (MQ=255)                                                                                                                      ccATCCTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCgatga  <  2:485132/140‑1 (MQ=255)                                                                                                                           cTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCa          >  2:639095/1‑127 (MQ=255)                                                                                                                           cTCATTTTTCACTACCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCa          <  1:639095/127‑1 (MQ=255)                                                                                                                                        |                                                                                                                          GGCGGCGTTGATATTTTCGCAAACCTTAAATGGCAGTAGTGAAGTTCCGCTATGGGATCGTCTCTTTGCGCCGTTAATGGCGGCAGCAGCTGGTGCACTTACTACCGGATTATTTTTCCATCCTCATTTTTCACTGCCCATTGCTCATTACGGACAGATGGAAATGACCGATATTCTCAGCGGTGCAATTGTCGCGGCGATTGCCATCGCAGCAGGGATGGTCGCCGTATGGTGCTTACCACGGTTGCACGCGATGA  >  NC_000913/2510124‑2510380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑