Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,529,127	G→A	G350G (GGC→GGT)	ligA ←	DNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,529,127	0	G	A	100.0%	177.0 / NA	51	G350G (GGC→GGT)	ligA	DNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

GGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGC  >  NC_000913/2528991‑2529261                                                                                                                                         |                                                                                                                                       ggCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCt                                                                                                                                     >  1:885698/1‑140 (MQ=255)     ttCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCaa                                                                                                                                 >  1:878552/1‑140 (MQ=255)     ttCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCaa                                                                                                                                 >  1:810645/1‑140 (MQ=255)      tCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAAc                                                                                                                                >  2:131525/1‑140 (MQ=255)       cAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACa                                                                                                                               <  2:1121587/140‑1 (MQ=255)           aaGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGa                                                                                                                           <  2:885698/140‑1 (MQ=255)           aaGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGa                                                                                                                           <  2:878552/140‑1 (MQ=255)           aaGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGa                                                                                                                           <  2:810645/140‑1 (MQ=255)           aaGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGa                                                                                                                           <  2:1157653/140‑1 (MQ=255)           aaGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGa                                                                                                                           <  1:900015/140‑1 (MQ=255)            aGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGAc                                                                                                                          >  1:736582/1‑140 (MQ=255)             gCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACa                                                                                                                         <  2:409110/140‑1 (MQ=255)              cACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCt                                                                                                                                     <  1:531949/127‑1 (MQ=255)              cACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCt                                                                                                                                     >  2:531949/1‑127 (MQ=255)                    gTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCa                                                                                                                  <  1:1035427/140‑1 (MQ=255)                        accaccTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGa                                                                                                                           >  1:1215179/1‑127 (MQ=255)                        accaccTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGa                                                                                                                           <  2:1215179/127‑1 (MQ=255)                        accaccTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGAcg                                                                                                              <  1:979225/140‑1 (MQ=255)                             cTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCaa                                                                                                         <  1:131525/140‑1 (MQ=255)                             cTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCaa                                                                                                         >  2:777687/1‑140 (MQ=255)                             cTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCaa                                                                                                         >  1:60334/1‑140 (MQ=255)                                        cGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATc                                                                                              >  2:464821/1‑140 (MQ=255)                                             ccAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGcc                                                                                         >  1:140018/1‑140 (MQ=255)                                               aGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAg                                                                                       <  1:208914/140‑1 (MQ=255)                                                   cggcgAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACg                                                                                   >  2:733184/1‑140 (MQ=255)                                                     gcgAATCACCGCTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGcc                                                                                 <  2:572118/140‑1 (MQ=255)                                                           caccacTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCt                                                                           >  1:228281/1‑140 (MQ=255)                                                                 tttATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACt                                                                     <  1:777687/140‑1 (MQ=255)                                                                        ccAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTc                                                              <  2:228281/140‑1 (MQ=255)                                                                        ccAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTc                                                              >  1:570758/1‑140 (MQ=255)                                                                         cAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCa                                                             <  1:733184/140‑1 (MQ=255)                                                                           aTGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACg                                                           <  2:311630/140‑1 (MQ=255)                                                                             gCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGca                                                         <  2:60334/140‑1 (MQ=255)                                                                                   aCCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAgg                                                   <  2:570758/140‑1 (MQ=255)                                                                                        gACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAg                                                    <  1:351560/134‑1 (MQ=255)                                                                                        gACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAg                                                    >  2:351560/1‑134 (MQ=255)                                                                                        gACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATc                                              <  2:388242/140‑1 (MQ=255)                                                                                            ttCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCt                                          <  1:493190/140‑1 (MQ=255)                                                                                                               tgtAAGGTTGCGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa                       <  2:576937/140‑1 (MQ=255)                                                                                                                         cGTTACTCACCAGCACACCTGCAGCATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa                       >  1:317445/1‑130 (MQ=255)                                                                                                                         cGTTACTCACCAGCACACCTGCAGCATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa                       <  2:317445/130‑1 (MQ=255)                                                                                                                         cGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGcc             >  1:950724/1‑140 (MQ=255)                                                                                                                         cGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGcc             >  2:458090/1‑140 (MQ=255)                                                                                                                         cGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGcc             >  1:897919/1‑140 (MQ=255)                                                                                                                         cGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGcc             >  2:627754/1‑140 (MQ=255)                                                                                                                         cGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGcc             >  1:578389/1‑140 (MQ=255)                                                                                                                         cGTTACTCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGcc             >  1:1104839/1‑140 (MQ=255)                                                                                                                               tCACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAgcgc       >  2:581743/1‑140 (MQ=255)                                                                                                                                cACCAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGcgcg      >  2:1144694/1‑140 (MQ=255)                                                                                                                                  ccAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCggg    <  2:91045/140‑1 (MQ=255)                                                                                                                                   cAGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCgggg   >  2:89607/1‑140 (MQ=255)                                                                                                                                    aGCACACCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGc  >  1:986504/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGC  >  NC_000913/2528991‑2529261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑