Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,567,343	A→G	G255G (GGT→GGC)	eutH ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,567,343	0	A	G	100.0%	195.1 / NA	54	G255G (GGT→GGC)	eutH	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/25);  total (29/25)

TTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGC  >  NC_000913/2567208‑2567478                                                                                                                                        |                                                                                                                                        tttttCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCgg                                                                                                                                     >  2:115582/1‑140 (MQ=255)     tCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGAt                                                                                                                                 >  1:145884/1‑140 (MQ=255)     tCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGAt                                                                                                                                 <  2:145884/140‑1 (MQ=255)           cAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTc                                                                                                                           >  1:970149/1‑140 (MQ=255)              cGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCa                                                                                                                        >  1:1146002/1‑140 (MQ=255)                     gcagcagcaCCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCa                                                                                                                               <  1:428604/126‑1 (MQ=255)                     gcagcagcaCCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCa                                                                                                                               >  2:428604/1‑126 (MQ=255)                      cagcagcaCCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCa                                                                                                                <  2:970149/140‑1 (MQ=255)                       agcagcaCCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAg                                                                                                               >  2:1185308/1‑140 (MQ=255)                                  cGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTa                                                                                                    >  1:769135/1‑140 (MQ=255)                                  cGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTa                                                                                                    >  1:734104/1‑140 (MQ=255)                                      tACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc                                                                                                <  2:769135/140‑1 (MQ=255)                                      tACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc                                                                                                <  1:562660/140‑1 (MQ=255)                                      tACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc                                                                                                <  2:491534/140‑1 (MQ=255)                                      tACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc                                                                                                <  2:734104/140‑1 (MQ=255)                                      tACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc                                                                                                <  1:115582/140‑1 (MQ=255)                                      tACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGc                                                                                                <  1:1145418/140‑1 (MQ=255)                                              tAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGc                                                                                          >  2:1119586/1‑138 (MQ=255)                                              tAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGc                                                                                          <  1:1119586/138‑1 (MQ=255)                                                 cAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGAc                                                                                     <  1:252932/140‑1 (MQ=255)                                                 cAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGAc                                                                                     <  1:160404/140‑1 (MQ=255)                                                 cAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGAc                                                                                     <  2:957207/140‑1 (MQ=255)                                                   gAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCg                                                                                   >  1:23949/1‑140 (MQ=255)                                                    aaCGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGc                                                                                          >  1:978275/1‑132 (MQ=255)                                                    aaCGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGc                                                                                          <  2:978275/132‑1 (MQ=255)                                                    aaCGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGa                                                                                  >  1:1030561/1‑140 (MQ=255)                                                     aCGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGAc                                                                                     <  1:1177962/136‑1 (MQ=255)                                                     aCGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGAc                                                                                     >  2:1177962/1‑136 (MQ=255)                                                      cGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAgg                                                                                <  1:476403/140‑1 (MQ=255)                                                           gagaTAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGAt                                                                           >  2:956926/1‑140 (MQ=255)                                                           gagaTAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGAt                                                                           <  2:546494/140‑1 (MQ=255)                                                                aGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATg                                                                      <  1:1185308/140‑1 (MQ=255)                                                                   aCCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCaa                                                                   >  2:159981/1‑140 (MQ=255)                                                                       aTAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGt                                                                              >  2:346721/1‑125 (MQ=255)                                                                       aTAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGt                                                                              <  1:346721/125‑1 (MQ=255)                                                                       aTAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAg                                                               >  2:744981/1‑140 (MQ=255)                                                                       aTAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAg                                                               >  1:264203/1‑140 (MQ=255)                                                                        tAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAgg                                                              >  2:8178/1‑140 (MQ=255)                                                                                 tGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg                                                     <  2:23949/140‑1 (MQ=255)                                                                                 tGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg                                                     <  2:863085/140‑1 (MQ=255)                                                                                 tGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg                                                     <  2:357035/140‑1 (MQ=255)                                                                                 tGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCg                                                     <  2:264203/140‑1 (MQ=255)                                                                                  ggCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGa                                                    <  1:956926/140‑1 (MQ=255)                                                                                             cctcaccGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAg                                         >  1:1045875/1‑140 (MQ=255)                                                                                             cctcaccGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAg                                         >  1:664758/1‑140 (MQ=255)                                                                                                   cGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTg                                   >  1:26916/1‑140 (MQ=255)                                                                                                          aTCGCCAGGGGCCATAAAGATAGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCAttt                            >  1:37478/1‑140 (MQ=255)                                                                                                                               aGGATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGcc       >  1:746808/1‑140 (MQ=255)                                                                                                                                 gATCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGcccc     >  2:734058/1‑140 (MQ=255)                                                                                                                                  aTCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGcc       >  2:616177/1‑137 (MQ=255)                                                                                                                                  aTCCAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGcc       <  1:616177/137‑1 (MQ=255)                                                                                                                                    ccAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAgc  >  2:670143/1‑140 (MQ=255)                                                                                                                                    ccAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAgc  >  2:81958/1‑140 (MQ=255)                                                                                                                                    ccAGGCCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAgc  >  1:411707/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGGAGATAGAACCGATAACTTCAATGGCGCGCATCACCTCACCGGGTTTATCGCCAGGGGCCATAAAGATAGGATCCAGACCGGGGATCAGTTCCCAGCCAAGCAGGAATTTCACTACCGCAGCGGCAAGACCGAGGGTGATCAATGCAACGAGGAATTTGGCGAAGATCTGGAAGCCGTTGATCATTTTTTCCGGGATGAATTTCAGCCCCAGC  >  NC_000913/2567208‑2567478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑