Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,631,526	A→G	I337T (ATC→ACC)	guaA ←	GMP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,631,526	0	A	G	100.0%	132.4 / NA	38	I337T (ATC→ACC)	guaA	GMP synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/18);  total (20/18)

CGTCTTTGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATC  >  NC_000913/2631389‑2631662                                                                                                                                          |                                                                                                                                         cGTCTTTGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTg                                                                                                                                        >  1:39814/1‑140 (MQ=255)       tGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGccc                                                                                                                                  >  2:617368/1‑140 (MQ=255)           cAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTgcg                                                                                                                              <  1:403581/140‑1 (MQ=255)              ctctTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTgg                                                                                                                                       <  1:621941/128‑1 (MQ=255)              ctctTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTgg                                                                                                                                       >  2:621941/1‑128 (MQ=255)              ctctTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCgcca                                                                                                                           >  1:721436/1‑140 (MQ=255)                       cGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCa                                                                                                                  <  1:1086144/140‑1 (MQ=255)                           tCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACg                                                                                                                <  1:314591/138‑1 (MQ=255)                           tCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACg                                                                                                                >  2:314591/1‑138 (MQ=255)                            cAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACg                                                                                                                >  1:597776/1‑137 (MQ=255)                            cAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACg                                                                                                                <  2:597776/137‑1 (MQ=255)                              aCCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTc                                                                                                           <  1:829077/140‑1 (MQ=255)                                     ccATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTc                                                                                                    <  2:724595/140‑1 (MQ=255)                                       aTCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAg                                                                                                  <  2:39814/140‑1 (MQ=255)                                         cTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAgcg                                                                                                >  1:608697/1‑140 (MQ=255)                                             aTCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGcttc                                                                                            >  1:587910/1‑140 (MQ=255)                                               ctctTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGcttctt                                                                                          >  2:1066101/1‑140 (MQ=255)                                                 ctTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAg                                                                                                  <  2:250889/130‑1 (MQ=255)                                                 ctTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAg                                                                                                  >  1:250889/1‑130 (MQ=255)                                                 ctTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCa                                                                                        <  1:1066101/140‑1 (MQ=255)                                                                      tgtgGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAAcg                                                                   <  1:717006/140‑1 (MQ=255)                                                                      tgtgGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAAcg                                                                   >  1:1140637/1‑140 (MQ=255)                                                                          gtgAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGAc                                                               >  1:530930/1‑140 (MQ=255)                                                                            gagaTTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCg                                                             <  2:608697/140‑1 (MQ=255)                                                                               aTTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCgatg                                                          >  2:830907/1‑140 (MQ=255)                                                                                  tgatgaCGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGAtt                                                       >  1:623327/1‑140 (MQ=255)                                                                                         gtgtGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCga                                                            <  2:161675/128‑1 (MQ=255)                                                                                         gtgtGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCga                                                            >  1:161675/1‑128 (MQ=255)                                                                                            tgCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTTATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTg                                             >  2:348230/1‑140 (MQ=255)                                                                                             gCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGc                                            >  1:346417/1‑140 (MQ=255)                                                                                                      gTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCTAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATc                                   >  2:61933/1‑140 (MQ=255)                                                                                                        tGCAGACGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGt                                 <  1:604381/140‑1 (MQ=255)                                                                                                             aCGCCGCAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCg                            <  2:346417/140‑1 (MQ=255)                                                                                                                 cgcAGATTCGATAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCgccag                        >  1:655543/1‑140 (MQ=255)                                                                                                                           aTAACGTCAGGGTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTTATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGa              <  1:348230/140‑1 (MQ=255)                                                                                                                                     ggTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGa    <  1:497363/140‑1 (MQ=255)                                                                                                                                      gTAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGAt   >  1:558169/1‑140 (MQ=255)                                                                                                                                       tAGGTGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGAtc  <  2:1140637/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CGTCTTTGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGATC  >  NC_000913/2631389‑2631662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑