Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,658,432	G→A	T168T (ACC→ACT)	hscA ←	iron‑sulfur cluster biosynthesis chaperone HscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,658,432	0	G	A	100.0%	138.6 / NA	40	T168T (ACC→ACT)	hscA	iron‑sulfur cluster biosynthesis chaperone HscA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/18);  total (22/18)

TCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGG  >  NC_000913/2658295‑2658560                                                                                                                                          |                                                                                                                                 tCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGt                                                                                                                                >  1:101131/1‑140 (MQ=255)   aaaCGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGc                                                                                                                              >  2:1179042/1‑140 (MQ=255)         cccGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGAc                                                                                                                        >  2:87402/1‑140 (MQ=255)          ccGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACg                                                                                                                       >  2:1203556/1‑140 (MQ=255)          ccGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACg                                                                                                                       >  1:185773/1‑140 (MQ=255)          ccGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACg                                                                                                                       >  1:762501/1‑140 (MQ=255)            gCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCt                                                                                                                     >  2:764541/1‑140 (MQ=255)               aCCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTggg                                                                                                                  >  1:716258/1‑140 (MQ=255)                ccGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGc                                                                                                                 <  2:716258/140‑1 (MQ=255)                       cATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCa                                                                                                          <  1:102813/140‑1 (MQ=255)                        aTAAACGGCGATCACGCCTTCCTGACCGGAATCCAGTCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCa                                                                                                                <  2:1136341/133‑1 (MQ=255)                        aTAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCa                                                                                                                >  1:1136341/1‑133 (MQ=255)                          aaaCGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAg                                                                                                       <  1:701806/140‑1 (MQ=255)                                  aTCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCACAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCgg                                                                                               >  1:618478/1‑140 (MQ=255)                                    cACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGaa                                                                                             <  1:87402/140‑1 (MQ=255)                                    cACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGaa                                                                                             <  1:764541/140‑1 (MQ=255)                                      cGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACg                                                                                           <  2:988128/140‑1 (MQ=255)                                        ccTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt                                                                                         <  2:185773/140‑1 (MQ=255)                                        ccTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt                                                                                         <  1:1083803/140‑1 (MQ=255)                                        ccTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt                                                                                         <  2:762501/140‑1 (MQ=255)                                        ccTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt                                                                                         <  1:1179042/140‑1 (MQ=255)                                        ccTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt                                                                                         >  1:15064/1‑140 (MQ=255)                                        ccTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt                                                                                         <  2:101131/140‑1 (MQ=255)                                             cTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGa                                                                                              <  1:572229/130‑1 (MQ=255)                                             cTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGa                                                                                              >  2:572229/1‑130 (MQ=255)                                             cTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGCCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATaa                                                                                    >  2:48068/1‑140 (MQ=255)                                                     aaTCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt                                                                                         >  1:887864/1‑127 (MQ=255)                                                     aaTCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGt                                                                                         <  2:887864/127‑1 (MQ=255)                                                      aTCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCAt                                                                           >  1:1184998/1‑140 (MQ=255)                                                                 aGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATGACTACACCATCCAGCTCGCCt                                                                >  2:1114766/1‑140 (MQ=255)                                                                        aGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAggg                                                         >  1:207139/1‑140 (MQ=255)                                                                         gCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGc                                                        <  1:717974/140‑1 (MQ=255)                                                                                              agtaagCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAg                                   <  1:48068/140‑1 (MQ=255)                                                                                              agtaagCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAg                                   <  1:1114766/140‑1 (MQ=255)                                                                                                     gcAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTg                            >  2:614720/1‑140 (MQ=255)                                                                                                          aCGTGAGGATCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGAt                       >  2:582613/1‑140 (MQ=255)                                                                                                          aCGTGAAGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGAt                       <  2:1184998/140‑1 (MQ=255)                                                                                                               aaGGCCCGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCg                  >  1:1197647/1‑140 (MQ=255)                                                                                                                   cccGCCAGACGCGCCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGa              >  2:317838/1‑140 (MQ=255)                                                                                                                             gcgcCGCGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCAcc    >  1:660854/1‑140 (MQ=255)                                                                                                                               gccgcGTCTTTAGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCgg  >  2:687434/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 TCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGTCAAAGTACGCCGGAACGGTGATAACTACACCATCCAGCTCGCCTGCCAGGGCTTCAGTTGCCCGCGCCGCCAGTGCTTTGAGGATGTCCGCAGAAACGCGCACCGG  >  NC_000913/2658295‑2658560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑