Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,177,509	(C)6→5	coding (402/630 nt)	nudF ←	ADP‑sugar pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,504	0	C	.	100.0%	122.3 / NA	29	coding (407/630 nt)	nudF	ADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/4);  total (25/4)

ACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCA  >  NC_000913/3177372‑3177643                                                                                                                                     |                                                                                                                                             aCCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGcccccggg                                                                                                                                       <  1:958975/140‑4 (MQ=255) aCCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGcccccggg                                                                                                                                       <  1:91358/140‑4 (MQ=255)            tGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGaa                                                                                                                           >  2:630779/1‑140 (MQ=255)                 gTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTa                                                                                                                      >  1:580163/1‑140 (MQ=255)                   tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAgg                                                                                                                             <  1:167808/131‑1 (MQ=255)                   tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAgg                                                                                                                             >  2:167808/1‑131 (MQ=255)                   tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAac                                                                                                                    >  1:469973/1‑140 (MQ=255)                   tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAac                                                                                                                    >  1:643754/1‑140 (MQ=255)                   tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAac                                                                                                                    >  1:880942/1‑140 (MQ=255)                       ggCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAgg                                                                                                                             >  2:727750/1‑127 (MQ=255)                       ggCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAgg                                                                                                                             <  1:727750/127‑1 (MQ=255)                                   gAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGt                                                                                                              >  2:803698/1‑130 (MQ=255)                                   gAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGt                                                                                                              <  1:803698/130‑1 (MQ=255)                                      cgcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAGACTTAACACCGGTTT‑GGTCCGTTTGa                                                                                                >  2:1030035/1‑140 (MQ=255)                                         gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTa                                                                                             <  1:1030035/140‑1 (MQ=255)                                          attatCTTCTTTTTCACCACCCAGCCGGTGAATCCCGTTTGGGGTCGTGGCGCCCCCTCCGCCCCCCATAATTGACGAACGCTCACTGGT‑CCCCCCGGGCTTGCCAGAAAACTTAAC‑CCGGTTTGGGTCCGTTTGACTAt                                                                                            <  2:880942/138‑1 (MQ=255)                                          aaTATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTAt                                                                                            >  1:793630/1‑140 (MQ=255)                                          aaTATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTAt                                                                                            >  1:836995/1‑140 (MQ=255)                                                   gTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTCT‑GGTCCGTTTGACTATCAGTCCCGc                                                                                   >  2:733375/1‑140 (MQ=255)                                                           cagccagcCCGTGAATCCCGCTTGCGGTCGGGGCGCCCCCTCCCCCCACCATAATTGACGAACGCTCCCTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACCCCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCaa                                                                           <  2:469973/140‑1 (MQ=255)                                                                     gtaaaTCCCTCTTGCGGTCGGGGCGTCCCCTTCCCCCACCATAATTGACGAACGCTCACTGGT‑CCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgcg                                                                 <  2:165978/137‑1 (MQ=255)                                                                        aaTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACg                                                              >  1:165978/1‑140 (MQ=255)                                                                              gCTTGGGGTCGGGGCGCCCATTCCCCCCACCATAATTGACGACCGCTCACTGGT‑CCCCCGGGGCTTGCCAGGAAACTTAACCCCGTTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc                                                        <  2:867520/140‑1 (MQ=255)                                                                              gCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc                                                        >  1:867520/1‑140 (MQ=255)                                                                                ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAt                                                      >  1:720935/1‑140 (MQ=255)                                                                                 tGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATc                                                     >  2:405375/1‑140 (MQ=255)                                                                                    ggTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCtat                                                  >  1:1012561/1‑138 (MQ=255)                                                                                          ggCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACt                                            >  1:115302/1‑140 (MQ=255)                                                                                                       cccACCATAATTGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCa                               >  1:33431/1‑140 (MQ=255)                                                                                                          ccaaTAATTGACGAACCCTCCCTGGT‑CCCCCCGGGCTTCCCAGAAAACTTAACCCCGTTTT‑GGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatc                            <  2:643754/137‑1 (MQ=255)                                                                                                                 ttGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCgg                     >  2:323020/1‑140 (MQ=255)                                                                                                                 ttGACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTCT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCgg                     <  1:733375/140‑1 (MQ=255)                                                                                                                   gACGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCa                   <  1:630779/140‑1 (MQ=255)                                                                                                                    gacagcccTCACTGGT‑CCCCCGGGGCTTGCCAGGAAACTTAACCCCGTTTT‑GGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa                  <  2:192976/133‑1 (MQ=255)                                                                                                                     cGAACGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAAc                 >  1:695783/1‑140 (MQ=255)                                                                                                                         cGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCAtc             >  1:479660/1‑140 (MQ=255)                                                                                                                         cGCTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCAtc             >  1:672677/1‑140 (MQ=255)                                                                                                                           cTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCAtctc           >  1:663558/1‑140 (MQ=255)                                                                                                                           cTCACTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCAtctc           >  2:828785/1‑140 (MQ=255)                                                                                                                              ccctggtccccccGGCCTTGCCAGGAAACTAACCCCCGGTTT‑GGTCCGTTTAACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCa         <  2:672677/133‑1 (MQ=255)                                                                                                                              ccTGGTG‑CCCCCGGGCTTGCCAGGAAACTTAACCCCGGTTT‑GGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAg        <  2:663558/139‑1 (MQ=255)                                                                                                                                     ccccccGGGCTTGCCAGAAAACTTACCCCCGGTTT‑GGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCa  <  2:1056682/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                             ACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTT‑GGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCA  >  NC_000913/3177372‑3177643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑