Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,237,643	(A)7→6	coding (10/2097 nt)	yjbH →	YjbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,237,637	0	A	.	100.0%	256.0 / NA	59	coding (4/2097 nt)	yjbH	YjbH family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (36/23);  total (36/23)

GCGTCACGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATGAAAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACGCCCACCGCGCG  >  NC_000913/4237508‑4237770                                                                                                                                  |                                                                                                                                      gCGTCACGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACAt                                                                                                                            <  1:1073459/140‑1 (MQ=255) gCGTCACGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACAt                                                                                                                            >  2:985292/1‑140 (MQ=255) gCGTCACGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACAt                                                                                                                            <  1:835012/140‑1 (MQ=255)   gTCACGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCt                                                                                                                          <  1:833636/140‑1 (MQ=255)      aCGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGGCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATGaaaaaagac                                                                                                                               >  2:1019328/1‑130 (MQ=255)      aCGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATGaaaaaagac                                                                                                                               <  1:1019328/133‑4 (MQ=255)      aCGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTgct                                                                                                                       >  2:883611/1‑140 (MQ=255)      aCGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTgct                                                                                                                       <  2:662799/140‑1 (MQ=255)      aCGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTgct                                                                                                                       >  1:134985/1‑140 (MQ=255)              ccGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTAc                                                                                                               <  1:208217/140‑1 (MQ=255)                 aTGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTgg                                                                                                            >  2:1030410/1‑140 (MQ=255)                 aTGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTgg                                                                                                            >  1:7180/1‑140 (MQ=255)                    cccGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGgcgc                                                                                                         <  2:608566/140‑1 (MQ=255)                      cGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTg                                                                                                       <  2:358282/140‑1 (MQ=255)                                atttatGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGca                                                                                             <  1:1030410/140‑1 (MQ=255)                                      gTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCtt                                                                                       >  1:76849/1‑140 (MQ=255)                                      gTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCtt                                                                                       >  1:901743/1‑140 (MQ=255)                                         ggCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCTCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCt                                                                                    >  2:1123210/1‑140 (MQ=255)                                          gCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAg                                                                                               <  1:534010/128‑1 (MQ=255)                                          gCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAg                                                                                               >  2:534010/1‑128 (MQ=255)                                          gCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTa                                                                                   >  1:975170/1‑140 (MQ=255)                                           ccTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTAc                                                                                  >  1:197154/1‑140 (MQ=255)                                           ccTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTAc                                                                                  >  1:387102/1‑140 (MQ=255)                                           ccTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTAc                                                                                  >  2:334526/1‑140 (MQ=255)                                                  gACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGaaa                                                                           <  2:975170/140‑1 (MQ=255)                                                           tGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTg                                                                  <  2:555360/140‑1 (MQ=255)                                                           tGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTg                                                                  >  1:879923/1‑140 (MQ=255)                                                            ggAGTGAGACGCCTGATGCTCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTgc                                                                 <  1:1123210/140‑1 (MQ=255)                                                                 gagaCGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCa                                                            >  2:1054653/1‑140 (MQ=255)                                                                  agaCGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCAt                                                           >  2:338665/1‑140 (MQ=255)                                                                  agaCGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCAt                                                           >  1:732570/1‑140 (MQ=255)                                                                         tGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCg                                                    >  1:596515/1‑140 (MQ=255)                                                                          gATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGt                                                   >  1:744534/1‑140 (MQ=255)                                                                            tGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCg                                                 >  1:19037/1‑140 (MQ=255)                                                                                  tAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGt                                                       >  1:453143/1‑128 (MQ=255)                                                                                  tAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGt                                                       <  2:453143/128‑1 (MQ=255)                                                                                  tAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCg                                           >  1:398565/1‑140 (MQ=255)                                                                                  tAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCg                                           >  2:457289/1‑140 (MQ=255)                                                                                         gACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCg                                    <  2:732570/140‑1 (MQ=255)                                                                                          aCATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCCGCGCCCATTGGTCCGTCGCAGTCGGATTTCgg                                   >  2:1157209/1‑140 (MQ=255)                                                                                                ttcAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGt                             <  2:7180/140‑1 (MQ=255)                                                                                                ttcAGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGt                             >  2:525757/1‑140 (MQ=255)                                                                                                   aGACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAgg                          <  1:527044/140‑1 (MQ=255)                                                                                                    gACTCTGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGa                         <  1:457289/140‑1 (MQ=255)                                                                                                      ctctGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGatt                       >  2:728139/1‑140 (MQ=255)                                                                                                        ctGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGattat                     <  1:728139/140‑1 (MQ=255)                                                                                                         tGACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGattatt                    >  2:1066773/1‑140 (MQ=255)                                                                                                          gACGCAGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGAttatta                   >  2:563611/1‑140 (MQ=255)                                                                                                               aGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTa                            >  2:357564/1‑126 (MQ=255)                                                                                                               aGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTa                            <  1:357564/126‑1 (MQ=255)                                                                                                               aGCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAAc              >  2:1103034/1‑140 (MQ=255)                                                                                                                gCGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACg             <  1:1103034/140‑1 (MQ=255)                                                                                                                 cGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAg                           >  2:831417/1‑125 (MQ=255)                                                                                                                 cGGATACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAg                           <  1:831417/125‑1 (MQ=255)                                                                                                                    aTACCTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACGCCCa         <  2:19037/140‑1 (MQ=255)                                                                                                                       ccTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACg             <  2:843290/133‑1 (MQ=255)                                                                                                                       ccTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACg             >  1:843290/1‑133 (MQ=255)                                                                                                                       ccTCAATAATG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACGCCCACcg      >  1:406408/1‑140 (MQ=255)                                                                                                                           aataatG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACGCCCACcgcgcg  >  1:1141189/1‑140 (MQ=255)                                                                                                                           aataatG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACGCCCACcgcgcg  >  2:417485/1‑140 (MQ=255)                                                                                                                           aataatG‑AAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACGCCCACcgcgcg  >  2:239620/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                      GCGTCACGTAGAGCCGATGCCCGGCAGCATTATTTATGTTGGCCTCGCGGACTCCGTCTGGAGTGAGACGCCTGATGCCCTTAACGCCGACATTCTTCAGACTCTGACGCAGCGGATACCTCAATAATGAAAAAAAGACATCTGCTTAGCTTACTGGCGCTGGGCATTAGCACAGCTTGCTACGGCGAAACATATCCTGCGCCCATTGGTCCGTCGCAGTCGGATTTCGGTGGCGTAGGATTATTACAAACGCCCACCGCGCG  >  NC_000913/4237508‑4237770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑