Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,367,230	(G)5→6	coding (305/717 nt)	yhcF →	DUF1120 domain‑containing protein YhcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,367,225	1	.	G	100.0%	199.2 / NA	55	S100R (AGT→AGG)	yhcF	DUF1120 domain‑containing protein YhcF
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (35/20);  total (35/20)

AATAGACTTTCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑GGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAA  >  NC_000913/3367088‑3367356                                                                                                                                           |                                                                                                                                     aaTAGACTTTCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑gg                                                                                                                                    <  1:819732/140‑1 (MQ=255)   tAGACTTTCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑g                                                                                                                                     >  1:117249/1‑137 (MQ=255)   tAGACTTTCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑g                                                                                                                                     <  2:117249/137‑1 (MQ=255)        tttCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑ggggggtca                                                                                                                             <  2:419927/140‑5 (MQ=255)         ttCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑ggggggtcag                                                                                                                            >  1:1153067/1‑135 (MQ=255)         ttCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑ggggggtcag                                                                                                                            >  2:537455/1‑135 (MQ=255)         ttCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑ggggggt                                                                                                                               >  1:579747/1‑135 (MQ=255)         ttCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑ggggggt                                                                                                                               <  2:579747/137‑3 (MQ=255)           cAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCagag                                                                                                                          <  1:816297/140‑1 (MQ=255)           cAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCagag                                                                                                                          <  1:201352/140‑1 (MQ=255)           cAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCagag                                                                                                                          >  1:139915/1‑140 (MQ=255)            aaCGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAgt                                                                                                                         <  1:139881/140‑1 (MQ=255)             aCGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAgtg                                                                                                                        >  2:1046604/1‑140 (MQ=255)                cagcaCTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAgtggtg                                                                                                                     <  1:770514/140‑1 (MQ=255)                               aTTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTaa                                                                                                      >  2:563703/1‑140 (MQ=255)                               aTTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTaa                                                                                                      >  2:105769/1‑140 (MQ=255)                                  ggAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATg                                                                                                   >  1:128907/1‑140 (MQ=255)                                  ggAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATg                                                                                                   >  2:156264/1‑140 (MQ=255)                                    aaCACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGc                                                                                                 >  2:171384/1‑140 (MQ=255)                                         gTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAgg                                                                                            >  2:563178/1‑140 (MQ=255)                                         gTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAgg                                                                                            <  1:563178/140‑1 (MQ=255)                                                  atatGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAg                                                                                   >  2:19873/1‑140 (MQ=255)                                                  atatGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAg                                                                                   >  2:776546/1‑140 (MQ=255)                                                       aCTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGa                                                                              >  1:454868/1‑140 (MQ=255)                                                       aCTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGa                                                                              >  1:451677/1‑140 (MQ=255)                                                       aCTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGa                                                                              >  2:262494/1‑140 (MQ=255)                                                       aCTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGa                                                                              >  2:301059/1‑140 (MQ=255)                                                           taACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAgg                                                                                            >  2:192547/1‑122 (MQ=255)                                                           taACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAgg                                                                                            <  1:192547/122‑1 (MQ=255)                                                            aaCTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAAcg                                                                         >  2:904266/1‑140 (MQ=255)                                                            aaCTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAAcg                                                                         <  1:301059/140‑1 (MQ=255)                                                            aaCTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGCTATATTAGCCAGACAAcg                                                                         >  2:158807/1‑140 (MQ=255)                                                               ttGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACgcgc                                                                      <  2:1153067/140‑1 (MQ=255)                                                                 gCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACa                                                                            <  2:261942/132‑1 (MQ=255)                                                                 gCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACa                                                                            >  1:261942/1‑132 (MQ=255)                                                                 gCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCtt                                                                    >  1:378669/1‑140 (MQ=255)                                                                 gCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCtt                                                                    >  1:194843/1‑140 (MQ=255)                                                                 gCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCtt                                                                    >  1:471461/1‑140 (MQ=255)                                                                   gAATCCGCTACCGGCATTGTGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGCGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTAt                                                                  >  1:452179/1‑140 (MQ=255)                                                                   gAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTAt                                                                  <  2:378669/140‑1 (MQ=255)                                                                         gCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCt                                                            >  1:14830/1‑140 (MQ=255)                                                                               ggCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGa                                                      <  1:19873/140‑1 (MQ=255)                                                                                gCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGaa                                                     >  2:514705/1‑140 (MQ=255)                                                                                              gCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGataata                                       <  1:336105/140‑1 (MQ=255)                                                                                               cTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGAtaataa                                      >  1:877254/1‑140 (MQ=255)                                                                                                tCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATaaa                                     >  1:620399/1‑140 (MQ=255)                                                                                                tCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATaaa                                     <  2:620399/140‑1 (MQ=255)                                                                                                      tACACGAATGGATAGTCTGACCTCCTGAAAAGATAGTGGGGGG‑TCAGAGTGGTGGAAAATATGCCTTAAATGGCGTAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATc                               <  2:661371/140‑1 (MQ=255)                                                                                                      tACACGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATc                               <  2:14830/140‑1 (MQ=255)                                                                                                        cacGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCgg                             <  2:390791/140‑1 (MQ=255)                                                                                                        cacGAATGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCgg                             <  2:194843/140‑1 (MQ=255)                                                                                                            aaTGGATAGTATGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGt                         >  1:432268/1‑140 (MQ=255)                                                                                                                     tATGACCACCGGAAAAGATAGT‑GGGGGATCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGTTAGTTATGCCgtg                >  2:624578/1‑140 (MQ=255)                                                                                                                       tGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGc                    >  2:1152098/1‑134 (MQ=255)                                                                                                                       tGACCACCGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGc                    <  1:1152098/134‑1 (MQ=255)                                                                                                                         accaccGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTaa            >  2:563815/1‑140 (MQ=255)                                                                                                                          ccaccGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGc                    >  1:813587/1‑131 (MQ=255)                                                                                                                          ccaccGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGc                    <  2:443095/131‑1 (MQ=255)                                                                                                                          ccaccGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGc                    >  1:443095/1‑131 (MQ=255)                                                                                                                          ccaccGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGc                    <  2:813587/131‑1 (MQ=255)                                                                                                                          ccaccGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAAt           >  1:59268/1‑140 (MQ=255)                                                                                                                              cGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGAt       >  2:282688/1‑140 (MQ=255)                                                                                                                              cGGAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGAt       >  1:709718/1‑140 (MQ=255)                                                                                                                               ggAAAAGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATa      >  1:44661/1‑140 (MQ=255)                                                                                                                                   aaGATAGTGGGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGcaa  <  1:262494/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     AATAGACTTTCAACGCAGCACTACACACAGATTGGAACACGTAATATTAATATGACTATAACTTGCGAATCCGCTACCGGCATTGCGATTACCGCTCGTGATACACGAATGGATAGTATGACCACCGGAAAAGATAGT‑GGGGG‑TCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAA  >  NC_000913/3367088‑3367356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑