| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 589,948 | (T)5→6 | coding (1007/2973 nt) | nfrA ← | bacteriophage N4 receptor, outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 589,943 | 1 | . | T | 84.6% | 112.9 / 14.1 | 39 | coding (1012/2973 nt) | nfrA | bacteriophage N4 receptor, outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (6/0); new base T (2/31); total (8/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.58e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑G‑TTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTT > NC_000913/589818‑590072 | aaGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGcg < 1:733854/140‑1 (MQ=255) aTTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGcgtcg < 1:804888/140‑1 (MQ=255) tttGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGcgtcgt < 1:521491/140‑1 (MQ=255) tGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGCG‑G‑TTTTTTTGCTGCGTCGTAc > 1:1151442/1‑140 (MQ=255) ccGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGGAGG‑TTTTTTCGCTGCGGCGTACTg > 2:432176/1‑140 (MQ=255) ccGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGCAGG‑TTTTTTTGCTGCGCGGTACTg > 2:804888/1‑140 (MQ=255) gTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGAcc < 2:694622/140‑1 (MQ=255) gCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGCAGG‑TTTTTTTGCTGCGTCGTACTGACCTTCttt > 1:931158/1‑140 (MQ=255) aTACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAAc < 1:699675/140‑1 (MQ=255) tACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGGAGG‑TTTTTTTGCTGCGTCGTACTGACCTTCTTTTTACa > 1:1072938/1‑140 (MQ=255) aCAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAg < 2:309979/140‑1 (MQ=255) cAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGGGGCGAGGA‑GTTTTTTTCGCTGCGTCGTACTGGCCTTCTTTTTACAGCAcc > 2:1026041/1‑140 (MQ=255) aTCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGGAGG‑TTTTTTCGCTGCGTCGTACTGACCTTCTTTTTACAACACCgg > 1:500492/1‑140 (MQ=255) tGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGCGGGCA‑GTTTTTTGCCCTGCCGCGCGCTGACCTTCTTTTTACAGCACCGGGAGc > 1:1103375/1‑140 (MQ=255) ccAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGGA‑GTTTTTTTTGCTGCGTCGTGATGTACTTCTTTTTTCAGACCCAGTAGCgg > 2:912516/1‑139 (MQ=255) ccAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGAGGAGG‑TTTTTTTGCTGCGTCGGACTGACCTTCTTTTTAAAGCACCCGTAGCgg > 2:111395/1‑139 (MQ=255) ccAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGt < 2:863061/140‑1 (MQ=255) gCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGcc < 2:287901/140‑1 (MQ=255) agagCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGCAGG‑TTTTTTCGCTGCGTCGTACTGACCCTCTTTTTACAACACCGGGAGCGTCGCGcccc > 2:860371/1‑138 (MQ=255) agagCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCcaa < 1:685438/140‑1 (MQ=255) agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCcaaca < 1:912516/140‑1 (MQ=255) agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCcaaca < 1:111395/140‑1 (MQ=255) gTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGCAGG‑TTTTTTTGCTGCGTCGTACTGACCTTCTTTTTACAGCACCGGGAGCGGCGCGCCAACAACATACAGGCgg > 2:673601/1‑140 (MQ=255) gTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGGGGGCAGG‑TTTTTTTGCTGCGGCGGACTGACCTTCTTTTTAAAACACCCGGAGCGTCGCGCCAACAACAAACTGGCgg > 2:1108028/1‑140 (MQ=255) aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTg < 2:903772/140‑1 (MQ=255) acaggCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCg < 1:201290/136‑1 (MQ=255) gCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGGGGGGGGCGGGCAGG‑TTTTTTTGCTGCGGCTTTCTGACCTTCTTTTTATAGCACCCGGGGCGGCGCCCCAACAACATACTGGCGGgtgttttggg > 2:180214/1‑134 (MQ=255) aCGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACt < 2:772202/140‑1 (MQ=255) gCTGACAGCATAACGCTCCTCAAGCATTTCGTTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGt < 2:63558/140‑1 (MQ=255) gCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGtat < 1:860371/140‑1 (MQ=255) tcAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACg < 2:261243/140‑1 (MQ=255) cAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGc < 1:877682/140‑1 (MQ=255) cAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGc < 1:432176/140‑1 (MQ=255) tttCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGTGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAAc < 1:341498/140‑1 (MQ=255) tttCATTGGC‑GGGGAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAAc < 1:414388/140‑1 (MQ=255) ggggAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAgcg < 1:784634/140‑1 (MQ=255) ggggAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAgcg < 1:677857/140‑1 (MQ=255) ggggAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAgcg < 1:64194/140‑1 (MQ=255) ggggAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAgcg < 1:207382/140‑1 (MQ=255) ggggAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAgcg < 1:180214/140‑1 (MQ=255) ggggAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAgcg < 2:931158/140‑1 (MQ=255) gggAGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAgcgc < 1:610633/140‑1 (MQ=255) aGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGt < 1:1026041/140‑1 (MQ=255) aGGGTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGt < 1:812008/140‑1 (MQ=255) ggTGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTAt < 2:1072938/140‑1 (MQ=255) tGGCGAGCA‑GTTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTTTCGGGCAAATGCACCCCATAAATCGCCAACGCCCGCAAGGGGGGAGCGCTGTtttt > 2:784634/1‑140 (MQ=255) | AAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGC‑GGGGAGGGTGGCGAGCA‑G‑TTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTATAATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTT > NC_000913/589818‑590072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |