Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,398	(C)5→6	intergenic (‑359/+9)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,393	1	.	C	100.0%	120.9 / NA	34	intergenic (‑354/+14)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (18/16);  total (18/16)

TATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTG‑CCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGAC  >  NC_000913/1351256‑1351518                                                                                                                                           |                                                                                                                              tatTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTG‑cc                                                                                                                             <  2:20717/140‑1 (MQ=255) tatTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTG‑cc                                                                                                                             <  2:862504/140‑1 (MQ=255)      ttACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTG‑cccccct                                                                                                                        <  1:152216/140‑3 (MQ=255)          cccAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGct                                                                                                                     >  2:947189/1‑140 (MQ=255)          cccAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGct                                                                                                                     <  1:947189/140‑1 (MQ=255)            cAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGctct                                                                                                                   >  1:818865/1‑140 (MQ=255)             aGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCtt                                                                                                                  >  1:166759/1‑140 (MQ=255)              gCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCttt                                                                                                                 <  2:818865/140‑1 (MQ=255)                      caGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGgaga                                                                                                         <  1:580224/140‑1 (MQ=255)                                   ccccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACcggcgg                                                                                            >  2:142177/1‑140 (MQ=255)                                   ccccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACcggcgg                                                                                            >  2:1080901/1‑140 (MQ=255)                                   ccccTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACcggcgg                                                                                            >  2:382144/1‑140 (MQ=255)                                        aaCCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAg                                                                                       >  1:1078793/1‑140 (MQ=255)                                           ccTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTc                                                                                    <  2:953710/140‑1 (MQ=255)                                                    gCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGaaa                                                                           >  1:168990/1‑139 (MQ=255)                                                         ggCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATtct                                                                      <  1:142177/140‑1 (MQ=255)                                                               gACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCa                                                                <  1:1080901/140‑1 (MQ=255)                                                                    ttCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGtc                                                           >  1:100970/1‑140 (MQ=255)                                                                    ttCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGt                                                            >  1:251019/1‑139 (MQ=255)                                                                    ttCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGt                                                            <  2:251019/139‑1 (MQ=255)                                                                      cAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGtctc                                                         <  1:1126961/140‑1 (MQ=255)                                                                          ccTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCa                                                     <  2:96185/140‑1 (MQ=255)                                                                              gCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAc                                                 >  2:386862/1‑140 (MQ=255)                                                                              gCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAc                                                 >  1:193474/1‑140 (MQ=255)                                                                                 gAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTCTTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAcaat                                              <  2:1078793/140‑1 (MQ=255)                                                                                                ccGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTcc                               >  2:689334/1‑140 (MQ=255)                                                                                                   tctcCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGa                            >  2:948169/1‑140 (MQ=255)                                                                                                       cATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACg                        <  2:166759/140‑1 (MQ=255)                                                                                                           ttttCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagc                    <  2:776941/140‑1 (MQ=255)                                                                                                            tttCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagca                   <  1:948169/140‑1 (MQ=255)                                                                                                            tttCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagca                   <  1:689334/140‑1 (MQ=255)                                                                                                            tttCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACgcagca                   <  1:386862/140‑1 (MQ=255)                                                                                                              tCACCACAACAGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACg                 >  2:242862/1‑140 (MQ=255)                                                                                                                     aacaGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTTTCCCGCACCGTCTCCCCATTACAAACAATCTCGTTATTCCAGAAAAGCAGCACCGTCCAGc          >  2:7775/1‑140 (MQ=255)                                                                                                                     aacaGCTATTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGc          >  2:459104/1‑140 (MQ=255)                                                                                                                            aTTCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAAAATTTTCCACCACCGTCTCCTCCTTAAAATCAATCTCGTTATTCCAAAAACCCAGCACGGTCCAGCCCTGCgc   >  2:1010484/1‑139 (MQ=255)                                                                                                                             ttCGGCTCGGTCTGCCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGAc  <  1:242862/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                              TATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTG‑CCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGTTATTCCAGAAACGCAGCACGGTCCAGCCCTGCGAC  >  NC_000913/1351256‑1351518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑