Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT31.6% 54.7 / 19.6 38intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (2/24);  new base T (12/0);  total (14/24)
Fisher's exact test for biased strand distribution p-value = 3.36e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206296‑3206566
                                                                                                                                           |                                                                                                                                   
gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTc                                                                                                                                     <  2:1091728/140‑1 (MQ=255)
  aaaaCATTGCCATTCCCTGCTCTGGCGCATTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                   >  1:423157/1‑140 (MQ=255)
  aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                   <  1:879501/140‑1 (MQ=255)
  aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                   >  2:198634/1‑140 (MQ=255)
           ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                          <  1:884906/140‑1 (MQ=255)
                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAaaaaaaaaaa                                                                                                                     >  2:30900/1‑140 (MQ=255)
                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAAAAAAAACAAAAAATggg                                                                                                                >  2:736671/1‑139 (MQ=255)
                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAaaaaaaaaaaaatga                                                                                                                >  1:667490/1‑135 (MQ=255)
                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAAAAaaaaacaaaaacacga                                                                                                                >  2:351868/1‑136 (MQ=255)
                       ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCTAcaaaaacaaaaaaatgagg                                                                                                              >  2:203873/1‑133 (MQ=255)
                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGc                                                                                                           >  1:917218/1‑140 (MQ=255)
                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAACAAAAAATGGAGTTGc                                                                                                           >  1:903823/1‑140 (MQ=255)
                               atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                      <  2:398106/140‑1 (MQ=255)
                               atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                      <  1:378103/140‑1 (MQ=255)
                                   tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                  <  1:924333/140‑1 (MQ=255)
                                   tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                  <  1:440813/140‑1 (MQ=255)
                                   tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                  <  2:917218/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                               <  2:413877/140‑1 (MQ=255)
                                       tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaa                                                                                              <  1:202191/140‑1 (MQ=255)
                                           aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGCATTTTTTCCAACAAAAACAAAAACACGAGTTGTCATGATGAGCGGAAgaa                                                                                          >  1:614909/1‑138 (MQ=255)
                                           aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                          <  2:355999/140‑1 (MQ=255)
                                           aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTTCCACAAAAAAAAAAAAATGAATTGCCATGATTTGGGAAAgaa                                                                                          >  1:1132374/1‑138 (MQ=255)
                                            ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGaaaaaaa                                                                                         >  2:202191/1‑136 (MQ=255)
                                                    ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                 <  1:38281/140‑1 (MQ=255)
                                                     tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                <  2:828228/140‑1 (MQ=255)
                                                              aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                       <  2:605179/140‑1 (MQ=255)
                                                               aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGACAAAAAAAAAAAAAGGAGTTGGCATGATGAGGGAAAGAAAAAAACAAAAAGGAGTGaaa                                                                      >  1:754420/1‑139 (MQ=255)
                                                                 tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGGGGTTCCATGATGAGGGAAAGTAATAAACAACAAGCAGTGAaaaa                                                                    >  2:94350/1‑140 (MQ=255)
                                                                 tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAGACATAAAAAAAAAATGGAGGTGGCATAATGAGCGGAAATAATAAACAAAAAGCAATGAaaaa                                                                    >  2:1017525/1‑140 (MQ=255)
                                                                  gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                   <  1:198634/140‑1 (MQ=255)
                                                                  gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                   <  2:423157/140‑1 (MQ=255)
                                                                   ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAACAAAAAATGGAGGTTCCATGATGAGCGGAAATAAAAAACAAAAAGCAGTGAAAAAga                                                                  >  2:1086065/1‑139 (MQ=255)
                                                                   ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTCCCAACAAAAACAAAAACCGACATTTCCACCATGAGCGAAAGTAAAAAACAACAAGCAGCGAATaaat                                                                  >  2:1018046/1‑138 (MQ=255)
                                                                           atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAAGAGGTGTCATGATGAGGGAAAAAAAAAAAAAACAAACAGGGAAAAAGATGACagaa                                                          >  2:256234/1‑139 (MQ=255)
                                                                            tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                         <  1:168448/140‑1 (MQ=255)
                                                                                  cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                   <  1:1086065/140‑1 (MQ=255)
                                                                                    gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGCTGCCCAGGTGGGGGAAAGGAATAAACAAAAGCCCATGGATAAACTGAAAGAAATTGTTGCt                                                 >  2:327861/1‑140 (MQ=255)
                                                                                     tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTAATTTTTTAGACAAAAAAAAAAAAATGGGATTCGATGGTGAGCGAAAATAAAAAACAAAAGGCGGTGAATAAATTGAAAGAGAttattccta                                                >  2:155605/1‑133 (MQ=255)
                                                                                     tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAGGAGGTGCCATGATGAGCGGAAGGAATAAAAAAAAGGCAGGGAATAAAATGACAGAGATTGTTGCTa                                                >  2:401499/1‑140 (MQ=255)
                                                                                       gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCATGATGGGGGAAAGTAAAAAACAACAAGCAGTGAAAAAATTGACAGAGAATGTTGCTaaa                                              >  2:898154/1‑139 (MQ=255)
                                                                                                   aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                  <  2:903823/140‑1 (MQ=255)
                                                                                                               tAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                      <  1:94350/140‑1 (MQ=255)
                                                                                                                aGTGGTAGTTTGTGGCGGTGATTTTTTCAAGACAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAAAAAGAAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTACTTTTACCGCCATGATTTCTACCCGaa                     <  2:1132374/140‑1 (MQ=255)
                                                                                                                 gtggtgTTTTGGGGCGTTGAATTTTGCAAGAAAAATAAAAAAGTGGGTGTGGCCATGATGAGCGAAAGAAATAAGCAAAAGGCGGTGAAAAGGTGGACGGATTTTGTCGCAATTTTTACCGCCATGATTTCTACCCGAAt                    <  2:614909/140‑1 (MQ=255)
                                                                                                                 gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                    <  1:401499/140‑1 (MQ=255)
                                                                                                                        tttGTGGCGGTGGATTTTTTCAGACAAATACAAAAAATGGAGGTGGCATGATGAGCGGAAGAAAAAAAAAACAGGCAGTGAATAAATTGAAAAAGATTGTCGCTAACTTTACCCCCATGATTTTTACCCCAAAGCCtgat             >  2:970975/1‑140 (MQ=255)
                                                                                                                         ttGTGGCGGTGAATTTTTCCAGAAAAATACAAAACCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg            <  1:351868/140‑1 (MQ=255)
                                                                                                                         ttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg            <  1:30900/140‑1 (MQ=255)
                                                                                                                         ttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg            <  1:736671/140‑1 (MQ=255)
                                                                                                                                  tGAATTTTTTCCGACAAAAAAAAAAAATGGAGTTGGCCTGATGAGCGGAAGTAATAAACAACAAGCAGGGAATAAATTGACAGAGATTGTTGCTAACTTTACCGCCATGATTTTTACCCGCATGCCTGATGACGgggggg   >  2:933553/1‑140 (MQ=255)
                                                                                                                                   gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa  <  1:160853/140‑1 (MQ=255)
                                                                                                                                   aaaTTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa  <  2:793160/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                   
GCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206296‑3206566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑