| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,404,215 | 0 | T | G | 27.3% | 86.0 / 14.1 | 44 | G244G (GGT→GGG) | yhdH | acrylyl‑CoA reductase |
| Reads supporting (aligned to +/- strand): ref base T (3/29); new base G (12/0); total (15/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATT > NC_000913/3404079‑3404352 | atgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGggtggt > 2:895374/1‑140 (MQ=255) ttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAct < 2:307577/140‑1 (MQ=255) ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑Tg < 1:999822/140‑1 (MQ=255) ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑Tg < 2:659058/140‑1 (MQ=255) aTCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTa < 2:26053/131‑1 (MQ=255) aTCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTa > 1:26053/1‑131 (MQ=255) tCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TTCCAAc > 2:999822/1‑140 (MQ=255) cGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TTCCAACCAc > 2:614682/1‑140 (MQ=255) tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGGAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TTCCAAACCCgg > 2:802963/1‑140 (MQ=255) tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TTCCCAcccccg > 2:222121/1‑136 (MQ=255) ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCAt < 1:793412/140‑1 (MQ=255) ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCAt < 1:895374/140‑1 (MQ=255) ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCAt < 1:614682/140‑1 (MQ=255) tGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGGGGCCGCCTGTGGTCTGGCGGGTGGGTTTACTCGTTCAAACCACggcgcg > 2:559349/1‑136 (MQ=255) aaaaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCAt < 1:802963/140‑1 (MQ=255) aaaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TTCCAAACACGGGCATGCCCtt > 2:669055/1‑140 (MQ=255) cAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TGCCCACCACGGGCATGCCAttttt > 2:784621/1‑138 (MQ=255) aaGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTAtt < 1:337757/140‑1 (MQ=255) tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TGCCAACCACGGGCATGCCATTTTTTCtt > 2:436469/1‑139 (MQ=255) ggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTa > 1:6521/1‑140 (MQ=255) ggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTa > 1:95902/1‑140 (MQ=255) tgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATg < 1:452979/140‑1 (MQ=255) ggggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGt < 1:1158731/140‑1 (MQ=255) gggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TGCCAACCACCGTCATGCCATTTATTCTGCGTAATGTc > 1:872312/1‑140 (MQ=255) ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TGCCAACCACGGGCATGCCATTTTTTCTGCGGAATGTcc > 2:464700/1‑140 (MQ=255) ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTC‑TGCCAACCACGGTCATGCCCTTTATTCTGCGTAATGTcc > 1:301608/1‑140 (MQ=255) ttGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTg > 1:26708/1‑140 (MQ=255) gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCa < 2:214313/140‑1 (MQ=255) gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCCGCGTAATGTCCGTTTGCa < 1:896525/140‑1 (MQ=255) acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCaa < 1:68101/140‑1 (MQ=255) cacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAg > 1:1049051/1‑140 (MQ=255) ccGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAggg < 2:757389/140‑1 (MQ=255) gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt < 1:1078287/140‑1 (MQ=255) gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt < 1:222121/140‑1 (MQ=255) ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGa < 2:95902/140‑1 (MQ=255) ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGa < 2:6521/140‑1 (MQ=255) gTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAAt < 1:784621/140‑1 (MQ=255) gTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAAt < 1:464700/140‑1 (MQ=255) gCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGa < 2:26708/140‑1 (MQ=255) cAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGGGGATTCAGTAATGACGCCACCAGAACGCCgcgc > 1:632894/1‑140 (MQ=255) gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TGCCAACCCCCGGCATGCCCTTTTTTCTTCGTAATGTGCGTTTGCAAAGGGGGGGTTTAGTAAAGACGCCACCAGAACGCCGCGCACAAGCCTGGCAg > 2:379422/1‑140 (MQ=255) gcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTC‑TTCCCACCACGGGCATGCCCTTTTTTTTGCGTTATGTTCGTTTTTAAGGGGGGGGTTTAGTAATGACGCCACCAGAAAGCCGCGCACAAGCCTGGGAGCGa > 2:551951/1‑140 (MQ=255) cTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGAc < 1:379422/140‑1 (MQ=255) cTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGAc < 2:872312/140‑1 (MQ=255) gcggcCTGTGGTCTGGCGGGTGGTTTTCCTC‑TGGCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGc < 2:271101/140‑1 (MQ=255) gcCTGTGGTCTGGCGGGCGGGTTTACTC‑TGCCAACCACGGGCATGCCATTTATTCTGCGTAATGGCCGGTTGCAAGGGGTGGATTCAGTAATGAGGCCACCAGAACGCCGGGCACAAGCCTGGCAGCGACTGGTCGCCGa > 2:484514/1‑140 (MQ=255) tgGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTAc < 1:436469/140‑1 (MQ=255) tGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa < 1:551951/140‑1 (MQ=255) tGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa < 2:494651/140‑1 (MQ=255) tGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa < 2:830712/140‑1 (MQ=255) tGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa > 1:830712/1‑140 (MQ=255) ggtggtTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt < 2:632894/140‑1 (MQ=255) | ATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTC‑TGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATT > NC_000913/3404079‑3404352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |