Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	306,909	T→C	E808E (GAA→GAG)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	306,909	0	T	C	100.0%	189.4 / NA	52	E808E (GAA→GAG)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/28);  total (24/28)

TTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCC  >  NC_000913/306775‑307036                                                                                                                                       |                                                                                                                                ttAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCag                                                                                                                            <  2:307241/140‑1 (MQ=255)     cgcgAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGcc                                                                                                                        >  1:1165/1‑140 (MQ=255)      gcgAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCa                                                                                                                       <  2:215347/140‑1 (MQ=255)          aaaTTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTc                                                                                                                   >  2:63397/1‑140 (MQ=255)              tATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAg                                                                                                               <  2:774455/140‑1 (MQ=255)                        attaGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGc                                                                                                     <  2:1165/140‑1 (MQ=255)                        attaGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGc                                                                                                     <  2:596859/140‑1 (MQ=255)                        attaGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGc                                                                                                     <  2:218905/140‑1 (MQ=255)                          tagtagGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCa                                                                                                   <  1:872943/140‑1 (MQ=255)                            gtagGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCCCCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACt                                                                                                 <  1:81622/140‑1 (MQ=255)                               ggTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTa                                                                                              >  1:1149300/1‑140 (MQ=255)                                gTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAg                                                                                             >  2:1145571/1‑140 (MQ=255)                                 ttAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCACGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGc                                                                                            >  1:282769/1‑140 (MQ=255)                                   aaCTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGa                                                                                          <  1:178654/140‑1 (MQ=255)                                    aCTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGaa                                                                                         >  2:596826/1‑140 (MQ=255)                                          aTTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGa                                                                                   <  1:852077/140‑1 (MQ=255)                                          aTTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGa                                                                                   >  2:866318/1‑140 (MQ=255)                                              tcttctcCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGTTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGt                                                                               <  1:63397/140‑1 (MQ=255)                                              tcttctcCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGt                                                                               <  1:1085378/140‑1 (MQ=255)                                                 tctcCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGA‑CCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTgggg                                                                           <  2:534139/140‑1 (MQ=255)                                                  ctcCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTgggg                                                                           <  1:909787/140‑1 (MQ=255)                                                  ctcCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTgggg                                                                           <  1:556949/140‑1 (MQ=255)                                                  ctcCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCCCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTgggg                                                                           <  2:119926/140‑1 (MQ=255)                                                        tCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTc                                                                     <  1:866318/140‑1 (MQ=255)                                                        tCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTc                                                                     >  2:280509/1‑140 (MQ=255)                                                          tGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCtt                                                                   <  1:1145571/140‑1 (MQ=255)                                                             gTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATc                                                                >  1:1057699/1‑140 (MQ=255)                                                                aCCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATcc                                                               <  1:746770/138‑1 (MQ=255)                                                                aCCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATcc                                                               <  1:72010/138‑1 (MQ=255)                                                                aCCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATcc                                                               >  2:746770/1‑138 (MQ=255)                                                                aCCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATcc                                                               >  2:72010/1‑138 (MQ=255)                                                                   gccgccCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTc                                                          <  2:141267/140‑1 (MQ=255)                                                                                      cGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGttt                                       >  2:1144886/1‑140 (MQ=255)                                                                                        cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTc                                     >  2:624533/1‑140 (MQ=255)                                                                                              aCCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCggt                               >  2:300211/1‑140 (MQ=255)                                                                                                  caTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCggttcgg                           >  2:960464/1‑140 (MQ=255)                                                                                                  caTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCggttcgg                           >  1:10881/1‑140 (MQ=255)                                                                                                  caTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCggttcgg                           >  1:618799/1‑140 (MQ=255)                                                                                                   aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCggttcggg                          >  2:660991/1‑140 (MQ=255)                                                                                                     cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                        >  1:643131/1‑140 (MQ=255)                                                                                                      aCCGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCggttcgggttc                       <  1:960464/140‑1 (MQ=255)                                                                                                       ccGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCggttcgggttcg                      <  1:300211/140‑1 (MQ=255)                                                                                                        cGACCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGgttcgggttcgg                     >  2:1127096/1‑140 (MQ=255)                                                                                                          aCCCAGACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTAGGGTTCGGcc                   >  1:75283/1‑140 (MQ=255)                                                                                                               gACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat              <  1:1144886/140‑1 (MQ=255)                                                                                                               gACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat              <  2:618799/140‑1 (MQ=255)                                                                                                               gACGGCACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat              <  1:1127096/140‑1 (MQ=255)                                                                                                                    cACCGCGCGCCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttg         >  1:209851/1‑140 (MQ=255)                                                                                                                        gcgcgcCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTaa     >  1:647703/1‑140 (MQ=255)                                                                                                                         cgcgcCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATg             >  2:1114652/1‑131 (MQ=255)                                                                                                                         cgcgcCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATg             <  1:1114652/131‑1 (MQ=255)                                                                                                                         cgcgcCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt    >  1:831377/1‑140 (MQ=255)                                                                                                                           cgcCTGGTTGAGCTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc  <  2:647703/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                TTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCC  >  NC_000913/306775‑307036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑