Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,019	T→C	V217V (GTA→GTG)	ftsP ←	cell division protein required during stress conditions

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,019	0	T	C	100.0%	177.8 / NA	50	V217V (GTA→GTG)	ftsP	cell division protein required during stress conditions
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/28);  total (22/28)

GGCAGGAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAA  >  NC_000913/3161882‑3162142                                                                                                                                          |                                                                                                                            ggcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCac                                                                                                                           >  1:144586/1‑140 (MQ=255) ggcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCac                                                                                                                           >  1:126850/1‑140 (MQ=255)  gcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGcaca                                                                                                                          <  1:371575/140‑1 (MQ=255)  gcaggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGcaca                                                                                                                          >  1:317265/1‑140 (MQ=255)   caggAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCacac                                                                                                                         <  2:126850/140‑1 (MQ=255)          cccTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAAc                                                                                                                  <  2:981250/140‑1 (MQ=255)            cTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACca                                                                                                                >  1:878417/1‑140 (MQ=255)            cTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACca                                                                                                                >  2:129430/1‑140 (MQ=255)             tGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACcag                                                                                                               <  1:617515/140‑1 (MQ=255)              gATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACcagc                                                                                                              <  1:46815/140‑1 (MQ=255)                         aTCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCAcc                                                                                                   >  1:108/1‑140 (MQ=255)                                 tAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAgc                                                                                           >  1:734257/1‑140 (MQ=255)                                        cGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCt                                                                                    >  1:968388/1‑140 (MQ=255)                                        cGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCt                                                                                    >  1:765293/1‑140 (MQ=255)                                          aCCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTc                                                                                  >  2:408851/1‑140 (MQ=255)                                          aCCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTc                                                                                  >  2:1134806/1‑140 (MQ=255)                                               cGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGt                                                                             <  2:968388/140‑1 (MQ=255)                                                  tCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCg                                                                          <  1:1150336/140‑1 (MQ=255)                                                         tAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTAtt                                                                   >  1:297600/1‑140 (MQ=255)                                                             tGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTgg                                                               <  2:108/140‑1 (MQ=255)                                                             tGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTgg                                                               <  1:1134806/140‑1 (MQ=255)                                                                tAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTg                                                                <  1:876959/136‑1 (MQ=255)                                                                tAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTg                                                                >  2:876959/1‑136 (MQ=255)                                                                tAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGt                                                            >  2:15163/1‑140 (MQ=255)                                                                tAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGt                                                            >  2:270249/1‑140 (MQ=255)                                                                 aGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTa                                                           >  1:1037915/1‑140 (MQ=255)                                                                  gCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTAc                                                          <  2:103083/140‑1 (MQ=255)                                                                   cgacgaGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTAcc                                                         >  2:78288/1‑140 (MQ=255)                                                                      cgaGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCCCACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTgg                                                               <  2:1037967/131‑1 (MQ=255)                                                                      cgaGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTgg                                                               >  1:1037967/1‑131 (MQ=255)                                                                                   cgTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGt                                         <  1:78288/140‑1 (MQ=255)                                                                                   cgTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGt                                         <  1:408851/140‑1 (MQ=255)                                                                                   cgTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGt                                         <  1:270249/140‑1 (MQ=255)                                                                                   cgTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGt                                         <  1:15163/140‑1 (MQ=255)                                                                                   cgTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGt                                         <  2:363690/140‑1 (MQ=255)                                                                                     ttCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGttt                                       >  2:226333/1‑140 (MQ=255)                                                                                       cagcagTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTAt                                     <  2:932500/140‑1 (MQ=255)                                                                                        agcagTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATc                                    <  2:878417/140‑1 (MQ=255)                                                                                          cagTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCt                                  <  2:765293/140‑1 (MQ=255)                                                                                          cagTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCt                                  <  2:734257/140‑1 (MQ=255)                                                                                                cAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATaa                            >  1:975074/1‑140 (MQ=255)                                                                                                  aGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATg                          >  1:156863/1‑140 (MQ=255)                                                                                                       aCCCAGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCgg                     >  1:499747/1‑140 (MQ=255)                                                                                                           aGCCACGCGAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGaaaa                 <  2:6477/140‑1 (MQ=255)                                                                                                                  cgAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCca          <  2:297600/140‑1 (MQ=255)                                                                                                                  cgAGACTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCca          <  2:950827/140‑1 (MQ=255)                                                                                                                     gaCTTCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCacac       <  2:975074/140‑1 (MQ=255)                                                                                                                         tCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATa   <  2:499747/140‑1 (MQ=255)                                                                                                                         tCAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATa   <  2:156863/140‑1 (MQ=255)                                                                                                                          cAACGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATaa  <  1:703868/140‑1 (MQ=255)                                                                                                                           aaCGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCca          <  2:1020078/131‑1 (MQ=255)                                                                                                                           aaCGTACGGGCTTTGCACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCca          >  1:1020078/1‑131 (MQ=255)                                                                                                                                          |                                                                                                                            GGCAGGAATCCCTGATCGCCAGAAATCACATGTAACGGGCGACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAA  >  NC_000913/3161882‑3162142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑