Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,170,207 T→C L127P (CTG→CCG)  qseB → DNA‑binding transcriptional activator QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,170,2070TC98.1% 181.2 / NA 52L127P (CTG→CCG) qseBDNA‑binding transcriptional activator QseB
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (26/25);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAA  >  NC_000913/3170072‑3170343
                                                                                                                                       |                                                                                                                                        
aTGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATTTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTgcgc                                                                                                                                      <  2:1139805/140‑1 (MQ=255)
       ggCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTa                                                                                                                               <  1:772344/140‑1 (MQ=255)
                 gtAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCt                                                                                                                     <  1:22612/140‑1 (MQ=255)
                     aaGGGCTGCGTCTGGGAGGTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGAc                                                                                                                 <  2:209441/140‑1 (MQ=255)
                          cTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCgg                                                                                                            >  1:796799/1‑140 (MQ=255)
                             cGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCCCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCaa                                                                                                         >  2:1155847/1‑140 (MQ=255)
                                cTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACg                                                                                                      <  2:869698/140‑1 (MQ=255)
                                 tGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGt                                                                                                     >  1:950319/1‑140 (MQ=255)
                                    gAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATc                                                                                                  >  1:234/1‑140 (MQ=255)
                                       cTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGcc                                                                                               >  2:674331/1‑140 (MQ=255)
                                       cTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGcc                                                                                               >  1:187588/1‑140 (MQ=255)
                                         gACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCAc                                                                                             >  1:97552/1‑140 (MQ=255)
                                                   tgtgTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCtggctggc                                                                                   <  2:466298/140‑1 (MQ=255)
                                                     tgtAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGa                                                                                 <  1:674331/140‑1 (MQ=255)
                                                     tgtAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGa                                                                                 <  2:187588/140‑1 (MQ=255)
                                                          aCCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCt                                                                            >  2:599059/1‑140 (MQ=255)
                                                               ttGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAATGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAaca                                                                       >  2:321095/1‑140 (MQ=255)
                                                               ttGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAaca                                                                       >  1:567488/1‑140 (MQ=255)
                                                               ttGCGTCGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAaca                                                                       >  2:787610/1‑140 (MQ=255)
                                                                      gATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAAc                                                                >  1:1057532/1‑140 (MQ=255)
                                                                                 ccgccAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAAttt                                                     >  2:271264/1‑140 (MQ=255)
                                                                                 ccgccAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAAttt                                                     >  2:254926/1‑140 (MQ=255)
                                                                                 ccgccAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAAttt                                                     >  2:178549/1‑140 (MQ=255)
                                                                                  cgccAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTg                                                    <  1:253054/140‑1 (MQ=255)
                                                                                    ccAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGcc                                                  <  1:599059/140‑1 (MQ=255)
                                                                                      aGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCCCGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCct                                                <  2:942104/140‑1 (MQ=255)
                                                                                       ggCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCctg                                               <  2:97552/140‑1 (MQ=255)
                                                                                        gCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCctgc                                              <  1:367256/140‑1 (MQ=255)
                                                                                         cTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGTCctgct                                             <  2:567488/140‑1 (MQ=255)
                                                                                                ctctGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTa                                      <  1:1140346/140‑1 (MQ=255)
                                                                                                ctctGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTa                                      <  1:223898/140‑1 (MQ=255)
                                                                                                  ctGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACt                                    >  2:518083/1‑140 (MQ=255)
                                                                                                  ctGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACt                                    >  2:386540/1‑140 (MQ=255)
                                                                                                  ctGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGGAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTAcc                                    >  2:695751/1‑139 (MQ=255)
                                                                                                   tGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTg                                   >  2:990199/1‑140 (MQ=255)
                                                                                                      tGCGCCGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATg                                >  1:719728/1‑140 (MQ=255)
                                                                                                          ccGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGAt                                 <  1:948423/135‑1 (MQ=255)
                                                                                                          ccGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGAt                                 >  2:948423/1‑135 (MQ=255)
                                                                                                          ccGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTa                            >  1:469175/1‑140 (MQ=255)
                                                                                                          ccGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTa                            >  1:150777/1‑140 (MQ=255)
                                                                                                           cGAACCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTaa                           <  1:178549/140‑1 (MQ=255)
                                                                                                             aaCCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGt                             >  1:337581/1‑136 (MQ=255)
                                                                                                             aaCCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGt                             <  2:337581/136‑1 (MQ=255)
                                                                                                             aaCCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACg                         <  1:990199/140‑1 (MQ=255)
                                                                                                              aCCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGc                        <  1:969442/140‑1 (MQ=255)
                                                                                                              aCCAACGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGc                        <  2:758428/140‑1 (MQ=255)
                                                                                                                 aaCGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCTCGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGCTgg                     <  2:719728/140‑1 (MQ=255)
                                                                                                                 aaCGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGCTgg                     <  2:69634/140‑1 (MQ=255)
                                                                                                                   cGGCCAGGCCAGCAACGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGCTGGTc                   <  1:254926/140‑1 (MQ=255)
                                                                                                                                aaCGAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGCTGGTCGGGTACTGTcgcg      <  1:221348/140‑1 (MQ=255)
                                                                                                                                   gAGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGCTGGTCGGGTACTGTCGCGCaa   >  2:1130619/1‑140 (MQ=255)
                                                                                                                                    aGCCGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGCTGGTCGGATACGGTCGCGCaaa  >  2:714912/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
ATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGAAGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGCTCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAA  >  NC_000913/3170072‑3170343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑