Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,236,458	A→G	I167V (ATC→GTC)	ygjP →	putative metal‑dependent hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,236,458	0	A	G	100.0%	303.2 / NA	83	I167V (ATC→GTC)	ygjP	putative metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/35);  total (48/35)

CGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTC  >  NC_000913/3236322‑3236594                                                                                                                                         |                                                                                                                                         cGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                       >  2:222776/1‑140 (MQ=255) cGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                       >  2:1006532/1‑140 (MQ=255) cGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCt                                                                                                                                       >  1:37427/1‑140 (MQ=255)  gAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTg                                                                                                                                      >  1:46053/1‑140 (MQ=255)   aGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCGCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGa                                                                                                                                     >  2:1056656/1‑140 (MQ=255)    gCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGaa                                                                                                                                    >  1:765554/1‑140 (MQ=255)    gCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGaa                                                                                                                                    >  1:1997/1‑140 (MQ=255)       ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAGCAAAGTCTGAAAat                                                                                                                                 <  1:411276/140‑1 (MQ=255)       ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAat                                                                                                                                 >  1:183259/1‑140 (MQ=255)        gcgcACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAatg                                                                                                                                <  1:1006532/140‑1 (MQ=255)            aCCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGACGt                                                                                                                            <  2:778095/140‑1 (MQ=255)             ccTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTc                                                                                                                           <  1:825341/140‑1 (MQ=255)             ccTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTc                                                                                                                           <  2:37427/140‑1 (MQ=255)             ccTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTc                                                                                                                           <  2:962963/140‑1 (MQ=255)                 aaGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGc                                                                                                                       <  2:833901/140‑1 (MQ=255)                 aaGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGc                                                                                                                       <  2:352935/140‑1 (MQ=255)                      gaAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTTCCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAt                                                                                                                  <  1:767507/140‑1 (MQ=255)                        aaGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtct                                                                                                                <  2:1140477/140‑1 (MQ=255)                        aaGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtct                                                                                                                >  2:1155950/1‑140 (MQ=255)                        aaGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtct                                                                                                                >  1:437696/1‑140 (MQ=255)                           agCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTCAtctttt                                                                                                             >  1:494461/1‑140 (MQ=255)                           agCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTAGACACCCGACTGTGGCTAAAGCAGTTGTAGCTTGAGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtctttt                                                                                                             >  2:21666/1‑140 (MQ=255)                                     aGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAg                                                                                                   >  2:392906/1‑140 (MQ=255)                                       cGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAgcg                                                                                                 >  2:170217/1‑140 (MQ=255)                                          tttACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCt                                                                                              >  2:277297/1‑140 (MQ=255)                                          tttACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCt                                                                                              >  1:907603/1‑140 (MQ=255)                                            tACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTaa                                                                                            >  2:32158/1‑140 (MQ=255)                                               cAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAAt                                                                                         <  2:46053/140‑1 (MQ=255)                                                   tgtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAc                                                                                     <  2:1997/140‑1 (MQ=255)                                                    gtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCg                                                                                       <  2:1062843/137‑1 (MQ=255)                                                    gtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCg                                                                                       >  1:1062843/1‑137 (MQ=255)                                                    gtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAcc                                                                                    >  2:927429/1‑140 (MQ=255)                                                    gtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAcc                                                                                    <  1:277297/140‑1 (MQ=255)                                                         gtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTcccc                                                                               >  1:815897/1‑140 (MQ=255)                                                           cATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTcccccc                                                                             <  1:32158/140‑1 (MQ=255)                                                           cATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTcccccc                                                                             <  1:222776/140‑1 (MQ=255)                                                            atatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCt                                                                            >  2:331472/1‑140 (MQ=255)                                                             tatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCttt                                                                           >  2:201736/1‑140 (MQ=255)                                                             tatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                           <  1:331472/140‑1 (MQ=255)                                                                 gAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGt                                                                       >  1:983488/1‑140 (MQ=255)                                                                  aaCCCCAGTACCACCAGCTTGAGTTCGACACCCGGCTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGtt                                                                      >  2:1029677/1‑140 (MQ=255)                                                                  aaCCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGtt                                                                      <  2:437696/140‑1 (MQ=255)                                                                   aCCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCTTTTCGTTa                                                                     <  1:201736/140‑1 (MQ=255)                                                                   aCCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTa                                                                     <  2:907603/140‑1 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAAGGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGAGCTCCCCCCTTTCGTTaa                                                                    >  2:1034832/1‑140 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGGTGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCt                                                                            >  2:180741/1‑132 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCt                                                                            >  1:165159/1‑132 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCt                                                                            <  2:165159/132‑1 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCt                                                                            <  1:180741/132‑1 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                    >  2:912961/1‑140 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                    >  2:622900/1‑140 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                    >  2:867864/1‑140 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                    >  1:1156227/1‑140 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                    >  1:1131279/1‑140 (MQ=255)                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                    >  1:602151/1‑140 (MQ=255)                                                                        aGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGAt                                                                >  1:1148069/1‑140 (MQ=255)                                                                          tACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATaa                                                              <  1:622900/140‑1 (MQ=255)                                                                            ccaccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACg                                                            >  2:1015267/1‑140 (MQ=255)                                                                               ccaGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGAcc                                                         >  1:92494/1‑140 (MQ=255)                                                                                caGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCa                                                        >  1:96336/1‑140 (MQ=255)                                                                                caGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCa                                                        <  1:927429/140‑1 (MQ=255)                                                                                 aGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAt                                                       <  1:1155950/140‑1 (MQ=255)                                                                                 aGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAt                                                       >  2:1007649/1‑140 (MQ=255)                                                                                      gAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtgctgc                                                  >  2:1110205/1‑140 (MQ=255)                                                                                                cccGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGc                                        <  2:1156227/140‑1 (MQ=255)                                                                                                     cTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGcc                                   <  1:1034832/140‑1 (MQ=255)                                                                                                        tgGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttc                                >  1:394690/1‑140 (MQ=255)                                                                                                          gCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttctt                              >  2:923293/1‑140 (MQ=255)                                                                                                               cGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttct                               <  1:1086203/134‑1 (MQ=255)                                                                                                               cGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttct                               >  2:1086203/1‑134 (MQ=255)                                                                                                               cGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCg                         >  1:702853/1‑140 (MQ=255)                                                                                                               cGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCg                         >  1:657456/1‑140 (MQ=255)                                                                                                                    ttGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttctt                              >  2:174293/1‑130 (MQ=255)                                                                                                                    ttGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttctt                              <  1:174293/130‑1 (MQ=255)                                                                                                                    ttGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTAttt                    >  2:234557/1‑140 (MQ=255)                                                                                                                    ttGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTAttt                    >  1:241836/1‑140 (MQ=255)                                                                                                                                 aGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg       <  2:765554/140‑1 (MQ=255)                                                                                                                                 aGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg       <  2:983488/140‑1 (MQ=255)                                                                                                                                 aGAACAAAGTCTGAAAATGATGTCAGGCGTCATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTCCTTCTCCGTATTTGTTTTTCTCGCCg       <  2:494461/140‑1 (MQ=255)                                                                                                                                  gAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCgg      <  2:241836/140‑1 (MQ=255)                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg       >  2:33436/1‑138 (MQ=255)                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg       <  1:33436/138‑1 (MQ=255)                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGa     >  1:834916/1‑140 (MQ=255)                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGa     <  2:1148069/140‑1 (MQ=255)                                                                                                                                      aaagTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGActc  <  2:394690/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTC  >  NC_000913/3236322‑3236594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑