Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,270,481	T→C	noncoding (112/377 nt)	rnpB ←	RNase P catalytic RNA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,481	0	T	C	100.0%	127.2 / NA	35	noncoding (112/377 nt)	rnpB	RNase P catalytic RNA component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/22);  total (13/22)

CGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑T‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGC  >  NC_000913/3270364‑3270617                                                                                                                        |                                                                                                                                          cGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCa                                                                                                                    <  1:650516/140‑1 (MQ=255)   tGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCAcc                                                                                                                  <  1:105583/140‑1 (MQ=255)   tGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCAcc                                                                                                                  <  2:276603/140‑1 (MQ=255)   tGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCAcc                                                                                                                  <  2:895314/140‑1 (MQ=255)    gCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTCC‑CCCCCCCAGGCGTTACCTGGCAcc                                                                                                                  <  2:1017021/140‑1 (MQ=255)      cACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTg                                                                                                               <  1:824206/140‑1 (MQ=255)                         cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCCCTGGTCGT‑GGGGT‑C‑CCCCCCCCCGCGCTTCCCGGCCCCCCCCCCCCTGGGGCCCCGGCtt                                                                                            >  2:865376/1‑140 (MQ=255)                         cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGG‑GGGGT‑C‑CCCCCCCCCGCGGTACCTGGCACCCCCCCCCATTGAGGCCCGGCtt                                                                                            >  2:669948/1‑140 (MQ=255)                                cgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctccc                                                                                     <  1:669948/140‑1 (MQ=255)                                            aCCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTTTTTCCCTCGTCGT‑GGGGT‑C‑CCCCCCCCCGCGGTTCCTGGCACCCCCCCCCCTGGAGCCCCGACCTTTCTCcccccccccccccc                                                                         >  2:851820/1‑136 (MQ=255)                                             gagacagaCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTCC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGtct                                                                         <  1:285147/133‑1 (MQ=255)                                               cTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTccc                                                                      <  2:466859/140‑1 (MQ=255)                                                        ttACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGTCGT‑GGGGT‑T‑CCCCCCCCGGCGGTACCTGGCCCCCCGCCCCATGGAGCCCGGACTTTCCTCCCCCCCCCCCCCCCCCCCCCCAGAgg                                                             >  2:995876/1‑140 (MQ=255)                                                          aCCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGTACTGGTCGG‑GGGTT‑TCCCCCCCCCCGCGGTTCCTGGCACCCCGCCCTCTGGAGCCCCGGCTTTCCTCCCCCCCCCCCCCCTCCCCCCCAGAggg                                                            >  2:952751/1‑139 (MQ=255)                                                                tCCCGCTTGCGCGGGCCATCGGCGGTTTGTTCTCTGTTGCACTCGTCGTGGGGTC‑C‑CCCCCCCCCGCGGTTCCTGGCACCCCCCCCCTTGGAGCCCCGGCTTTTCTCCCCCCCCCCCCTCTCCCCCCAAGAAggcgaagc                                                      >  2:405590/1‑137 (MQ=255)                                                                    gCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAg                                                 <  1:216258/140‑1 (MQ=255)                                                                          gcgGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTCC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGTACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAgcggcg                                            <  1:966277/140‑1 (MQ=255)                                                                          gcgGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGa                                           <  1:865376/140‑1 (MQ=255)                                                                              gCCATCGGCGGTTTTGTTTCTTTTGTCCTGGTCGTGGGGTT‑C‑CCCCCCCCCGGGGTACCTGGGACCCCGCCCCATGGGGGCCGGGCTTTTCTCCCCCCCCCCCCCCTCCCCCCCAGAGGACGACGACGAAgcgggggcgg                                        >  2:283501/1‑134 (MQ=255)                                                                                            gCTCTCTGTTGCACTGGTCGT‑GGGTTCC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCg                          <  1:283501/140‑1 (MQ=255)                                                                                                tctGTTGCACTGGTCGT‑GGGTTCC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgc                        >  1:787102/1‑138 (MQ=255)                                                                                                tctGTTGCACTGGTCGT‑GGGTTCC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgc                        <  2:787102/138‑1 (MQ=255)                                                                                                tctGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcg                     <  2:377533/140‑1 (MQ=255)                                                                                                 ctGTTGCACTGGTCGT‑GGGTTCC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcg                     <  1:952751/140‑1 (MQ=255)                                                                                                 ctGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                    <  1:995876/140‑1 (MQ=255)                                                                                                  tGTTGCACTGGTCGT‑GGGTTCC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                    <  1:430293/140‑1 (MQ=255)                                                                                                  tGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcg                     <  2:404517/138‑1 (MQ=255)                                                                                                  tGTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcg                     >  1:404517/1‑138 (MQ=255)                                                                                                   gTTGCACTGGTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAg                  >  2:857177/1‑140 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                    <  1:1140684/129‑1 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                    >  2:1150653/1‑129 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                    >  2:1140684/1‑129 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc                    <  1:1150653/129‑1 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAggg         >  2:282993/1‑140 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAggg         >  2:564315/1‑140 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAggg         >  2:1095920/1‑140 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAggg         >  1:764885/1‑140 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAggg         >  1:70281/1‑140 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAggg         >  1:220907/1‑140 (MQ=255)                                                                                                            gTCGT‑GGGTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAggg         >  2:992906/1‑140 (MQ=255)                                                                                                                  gggTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTgc    >  1:637249/1‑140 (MQ=255)                                                                                                                    gTT‑C‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTgcgg  <  2:1026908/140‑2 (MQ=255)                                                                                                                        |                                                                                                                                          CGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTT‑T‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGC  >  NC_000913/3270364‑3270617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑