Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,380,178	A→G	intergenic (‑181/‑13)	zapE ← / → yhcB	cell division factor ZapE with ATPase activity/conserved protein YhcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,380,178	0	A	G	100.0%	226.9 / NA	61	intergenic (‑181/‑13)	zapE/yhcB	cell division factor ZapE with ATPase activity/conserved protein YhcB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/33);  total (28/33)

GTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAA  >  NC_000913/3380043‑3380308                                                                                                                                        |                                                                                                                                   gTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCggg                                                                                                                                <  2:774817/140‑1 (MQ=255)  ttGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGga                                                                                                                               >  1:935811/1‑140 (MQ=255)   tGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGgag                                                                                                                              <  2:413606/140‑1 (MQ=255)     aCGAAAAAAAGGCCGTTCTACACTACACGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGAt                                                                                                                            >  1:899528/1‑140 (MQ=255)           aaaaGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCAt                                                                                                                      <  1:268019/140‑1 (MQ=255)                 ccGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTg                                                                                                                <  2:899528/140‑1 (MQ=255)                          acTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATgc                                                                                                       >  2:50655/1‑140 (MQ=255)                           cTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATgcg                                                                                                      <  1:50655/140‑1 (MQ=255)                              cgcgATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTa                                                                                                   >  1:100737/1‑140 (MQ=255)                              cgcgATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTa                                                                                                   >  1:1006871/1‑140 (MQ=255)                                     tGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGt                                                                                            >  1:232208/1‑140 (MQ=255)                                        aGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAg                                                                                         >  1:538308/1‑140 (MQ=255)                                           cGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATg                                                                                                        <  1:862824/122‑1 (MQ=255)                                           cGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATg                                                                                                        >  2:862824/1‑122 (MQ=255)                                                ttCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGc                                                                                 <  2:614505/140‑1 (MQ=255)                                                ttCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGc                                                                                 <  2:232208/140‑1 (MQ=255)                                                 tCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGca                                                                                <  2:935811/140‑1 (MQ=255)                                                  ccACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGcat                                                                               <  2:210422/140‑1 (MQ=255)                                                        cTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCattat                                                                         <  2:112836/140‑1 (MQ=255)                                                        cTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCattat                                                                         <  2:100737/140‑1 (MQ=255)                                                          gtgGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCGACGGAAACAAAAGACAGCGTGAGATGTTCATGACCTGGGAATATACGCTAATTGGGTTAGTCGTCGTCATCATTATTg                                                                       <  2:182023/140‑1 (MQ=255)                                                           tgGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCattat                                                                         >  1:185778/1‑137 (MQ=255)                                                           tgGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCattat                                                                         <  2:185778/137‑1 (MQ=255)                                                           tgGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTgg                                                                      >  1:242711/1‑140 (MQ=255)                                                                ttAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGc                                                                                 >  1:61555/1‑124 (MQ=255)                                                                ttAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGc                                                                                 <  2:61555/124‑1 (MQ=255)                                                                ttAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGccg                                                                 >  1:450859/1‑138 (MQ=255)                                                                  aaCGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCtgtg                                                               >  2:318258/1‑140 (MQ=255)                                                                       ttATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCAt                                                          <  2:920210/140‑1 (MQ=255)                                                                       ttATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCAt                                                          <  1:318258/140‑1 (MQ=255)                                                                          taGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCg                                                       <  1:883014/140‑1 (MQ=255)                                                                                cATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTgg                                                 <  1:286265/140‑1 (MQ=255)                                                                                 aTAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCa                                                           <  2:1027711/129‑1 (MQ=255)                                                                                 aTAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCa                                                           >  1:1027711/1‑129 (MQ=255)                                                                                 aTAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGt                                                >  2:114308/1‑140 (MQ=255)                                                                                 aTAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGt                                                <  2:715192/140‑1 (MQ=255)                                                                                 aTAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGt                                                >  1:715192/1‑140 (MQ=255)                                                                                     tCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTgg                                                 <  2:544548/135‑1 (MQ=255)                                                                                     tCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTgg                                                 >  1:544548/1‑135 (MQ=255)                                                                                     tCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATc                                            >  1:1141752/1‑140 (MQ=255)                                                                                       aGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCg                                           >  1:179568/1‑139 (MQ=255)                                                                                       aGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCg                                           <  2:179568/139‑1 (MQ=255)                                                                                         ccGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTaa                                        >  1:237880/1‑140 (MQ=255)                                                                                           gCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAAc                                      <  2:538308/140‑1 (MQ=255)                                                                                              ggCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTaa                                              >  1:813100/1‑129 (MQ=255)                                                                                              ggCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTaa                                              <  2:813100/129‑1 (MQ=255)                                                                                               gCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACg                                  <  2:237880/140‑1 (MQ=255)                                                                                               gCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACg                                  <  1:114308/140‑1 (MQ=255)                                                                                                     aGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCa                            <  2:1141752/140‑1 (MQ=255)                                                                                                     aGCCTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCa                            <  1:826322/140‑1 (MQ=255)                                                                                                       ccTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCaa                           <  2:127439/139‑1 (MQ=255)                                                                                                       ccTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCaa                           >  1:127439/1‑139 (MQ=255)                                                                                                       ccTGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAAc                          <  2:698262/140‑1 (MQ=255)                                                                                                         tGAAGCCAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAg                        >  1:845293/1‑140 (MQ=255)                                                                                                               cAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAgg                       <  2:80684/135‑1 (MQ=255)                                                                                                               cAACACCCTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAgg                       >  1:80684/1‑135 (MQ=255)                                                                                                                      cTACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACg           >  2:990759/1‑140 (MQ=255)                                                                                                                       tACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGa          >  2:573438/1‑140 (MQ=255)                                                                                                                       tACGGAAACAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGa          >  2:1163481/1‑140 (MQ=255)                                                                                                                              aCAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGaa   <  1:573438/140‑1 (MQ=255)                                                                                                                              aCAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGaa   <  1:1163481/140‑1 (MQ=255)                                                                                                                               cAAAAGACAGCGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGaaa  >  2:943978/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   GTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAA  >  NC_000913/3380043‑3380308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑