Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,463,948	T→C	R9R (CGT→CGC)	gspL →	Type II secretion system protein GspL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,463,948	0	T	C	100.0%	320.4 / NA	86	R9R (CGT→CGC)	gspL	Type II secretion system protein GspL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/45);  total (41/45)

CAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGTTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTT  >  NC_000913/3463811‑3464083                                                                                                                                          |                                                                                                                                        cAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGctc                                                                                                                                       >  1:63789/1‑140 (MQ=255)      acgaACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCt                                                                                                                                  <  2:569762/140‑1 (MQ=255)        gaACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCg                                                                                                                                >  1:484728/1‑140 (MQ=255)         aaCAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGa                                                                                                                               <  1:816813/140‑1 (MQ=255)          aCAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGAc                                                                                                                              <  2:325919/140‑1 (MQ=255)          aCAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGAc                                                                                                                              <  2:728138/140‑1 (MQ=255)          aCAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGAc                                                                                                                              <  2:334004/140‑1 (MQ=255)           cAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACg                                                                                                                             <  1:643444/140‑1 (MQ=255)           cAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACg                                                                                                                             <  2:484728/140‑1 (MQ=255)           cAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACg                                                                                                                             <  1:857358/140‑1 (MQ=255)                ccAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCg                                                                                                                        <  2:15449/140‑1 (MQ=255)                  aGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTa                                                                                                                      <  1:382077/140‑1 (MQ=255)                   gggATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTaa                                                                                                                     >  1:127935/1‑140 (MQ=255)                      aTGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCa                                                                                                                  >  1:970350/1‑140 (MQ=255)                      aTGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCa                                                                                                                  <  2:127935/140‑1 (MQ=255)                        ggCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCAtt                                                                                                                >  2:322492/1‑140 (MQ=255)                        ggCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCAtt                                                                                                                >  2:1106924/1‑140 (MQ=255)                        ggCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCAtt                                                                                                                >  2:993328/1‑140 (MQ=255)                        ggCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCAtt                                                                                                                >  2:59810/1‑140 (MQ=255)                        ggCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCAtt                                                                                                                >  2:975893/1‑140 (MQ=255)                        ggCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCAtt                                                                                                                >  1:1103224/1‑140 (MQ=255)                        ggCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCAtt                                                                                                                >  2:870095/1‑140 (MQ=255)                          cAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTgg                                                                                                              <  2:1103224/140‑1 (MQ=255)                            aaGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGa                                                                                                            <  2:696962/140‑1 (MQ=255)                            aaGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGa                                                                                                            <  2:972343/140‑1 (MQ=255)                            aaGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGa                                                                                                            <  1:528356/140‑1 (MQ=255)                               ccATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATg                                                                                                         >  1:26357/1‑140 (MQ=255)                                  tatTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGAt                                                                                                      >  2:130980/1‑140 (MQ=255)                                  tatTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACACTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGAt                                                                                                      >  1:1067566/1‑140 (MQ=255)                                               cGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCa                                                                                         <  1:870095/140‑1 (MQ=255)                                                 tAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGa                                                                                       <  1:59810/140‑1 (MQ=255)                                                       gcagAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGt                                                                                 <  1:130980/140‑1 (MQ=255)                                                       gcagAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGt                                                                                 <  1:975893/140‑1 (MQ=255)                                                       gcagAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGt                                                                                 <  2:970350/140‑1 (MQ=255)                                                        cagAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTa                                                                                >  2:387424/1‑140 (MQ=255)                                                        cagAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTa                                                                                <  1:924904/140‑1 (MQ=255)                                                        cagAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTa                                                                                >  1:84548/1‑140 (MQ=255)                                                        cagAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTa                                                                                >  1:305535/1‑140 (MQ=255)                                                           agCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTAt                                                                               <  1:569787/138‑1 (MQ=255)                                                           agCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTAt                                                                               >  2:569787/1‑138 (MQ=255)                                                             cgcCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTc                                                                           >  2:348967/1‑140 (MQ=255)                                                              gcCCAAATCTATCGGCGTACACTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCg                                                                          <  2:1067566/140‑1 (MQ=255)                                                               cccAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCgg                                                                         >  1:541057/1‑140 (MQ=255)                                                                        aTCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGc                                                                <  2:681862/140‑1 (MQ=255)                                                                         tCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCt                                                               <  2:63789/140‑1 (MQ=255)                                                                               tACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTGCATACGCTCACGGa                                                         <  1:1106924/140‑1 (MQ=255)                                                                                    tGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATc                                                    <  1:591413/140‑1 (MQ=255)                                                                                     gCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCt                                                   <  2:805781/140‑1 (MQ=255)                                                                                         ctctACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCg                                               >  2:360622/1‑140 (MQ=255)                                                                                             actaATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTgg                                           <  2:305535/140‑1 (MQ=255)                                                                                                aaTCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGt                                        >  2:107476/1‑140 (MQ=255)                                                                                                aaTCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGt                                        >  1:1013899/1‑140 (MQ=255)                                                                                                   cATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGc                                     >  2:19955/1‑140 (MQ=255)                                                                                                   cATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGc                                     >  2:553427/1‑140 (MQ=255)                                                                                                    aTAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCt                                    >  2:749327/1‑140 (MQ=255)                                                                                                     tAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTg                                   <  1:19955/140‑1 (MQ=255)                                                                                                      aaGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTgg                                  <  1:1014256/140‑1 (MQ=255)                                                                                                      aaGGATCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTgg                                  >  1:725062/1‑140 (MQ=255)                                                                                                        ggACCGTGAAGCCTGATTCGGTGATGGTCCTACGCTCCACCTCGACGCTGCGGAAACATTTGGAATGGCTGACGTTCTCCGCAGACACCGTATCTTCGGTACATACGCACACGGACGATCTTCCGCTGGAGTCGCTCGcc                                >  2:772718/1‑140 (MQ=255)                                                                                                           tCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGAt                             >  1:844077/1‑140 (MQ=255)                                                                                                           tCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGAt                             <  1:1138836/140‑1 (MQ=255)                                                                                                           tCGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGAt                             >  1:271099/1‑140 (MQ=255)                                                                                                           tCGTGATGCCTGAATCGTTGATGGTCATACGCACTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGAt                             >  2:622693/1‑140 (MQ=255)                                                                                                            cGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTg                                   <  1:949257/133‑1 (MQ=255)                                                                                                            cGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTg                                   >  2:949257/1‑133 (MQ=255)                                                                                                            cGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATc                            >  1:918974/1‑140 (MQ=255)                                                                                                            cGTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATc                            >  2:259179/1‑140 (MQ=255)                                                                                                             gTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAg                                         >  2:169516/1‑126 (MQ=255)                                                                                                             gTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAg                                         <  1:169516/126‑1 (MQ=255)                                                                                                             gTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCa                           >  2:605451/1‑140 (MQ=255)                                                                                                             gTGATGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCa                           >  1:230027/1‑140 (MQ=255)                                                                                                                aTGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGcc                        >  1:735192/1‑140 (MQ=255)                                                                                                                aTGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGcc                        <  2:735192/140‑1 (MQ=255)                                                                                                                 tGCCTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGccc                       <  2:84548/140‑1 (MQ=255)                                                                                                                   ccTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTGAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCgg                     >  2:723373/1‑140 (MQ=255)                                                                                                                    cTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGt                    >  2:776597/1‑140 (MQ=255)                                                                                                                    cTGAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGt                    >  2:345852/1‑140 (MQ=255)                                                                                                                      gAATCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGc                  <  1:360622/140‑1 (MQ=255)                                                                                                                       aaTCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGcc                 >  1:239007/1‑140 (MQ=255)                                                                                                                         tCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCgg               <  2:918974/140‑1 (MQ=255)                                                                                                                         tCGTTGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCgg               <  2:1013899/140‑1 (MQ=255)                                                                                                                            ttGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCgg                     >  2:1163045/1‑131 (MQ=255)                                                                                                                            ttGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCgg                     <  1:1163045/131‑1 (MQ=255)                                                                                                                            ttGATGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGaaa            <  1:466175/140‑1 (MQ=255)                                                                                                                                tGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGtt        <  1:345852/140‑1 (MQ=255)                                                                                                                                tGGTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGtt        <  1:776597/140‑1 (MQ=255)                                                                                                                                 ggTCATACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTc       <  2:747240/140‑1 (MQ=255)                                                                                                                                      tACGCTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCtt  <  1:344270/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CAGCGACGAACAATCCCAGGGATGGCAAAGCCATATTTTTTTCAACCGTAAACAGCAGAGCGCCCAAATCTATCGGCGTACGCTGCAACTCTACTAATCATAAGGATCGTGATGCCTGAATCGTTGATGGTCATACGTTCTTCCTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTT  >  NC_000913/3463811‑3464083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑