Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,538,968	T→C	G528G (GGT→GGC)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,538,968	0	T	C	100.0%	200.9 / NA	55	G528G (GGT→GGC)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

GCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTA  >  NC_000913/3538837‑3539102                                                                                                                                    |                                                                                                                                       gCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCa                                                                                                                                <  1:315373/140‑1 (MQ=255)   agtagtAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGa                                                                                                                              >  2:1087633/1‑140 (MQ=255)    gtagtaGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGaa                                                                                                                             <  1:1039403/140‑1 (MQ=255)      agtagAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAAcc                                                                                                                           <  1:578269/140‑1 (MQ=255)            agaCTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGc                                                                                                                     >  2:196222/1‑140 (MQ=255)             gaCTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCa                                                                                                                    >  1:1139594/1‑140 (MQ=255)               cTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAg                                                                                                                               <  2:605482/127‑1 (MQ=255)               cTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAg                                                                                                                               >  1:605482/1‑127 (MQ=255)               cTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAAc                                                                                                                  >  2:553073/1‑140 (MQ=255)                 gCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCa                                                                                                                    <  2:231002/136‑1 (MQ=255)                 gCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCa                                                                                                                    >  1:231002/1‑136 (MQ=255)                  cGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGt                                                                                                               >  1:271638/1‑140 (MQ=255)                  cGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGt                                                                                                               >  1:591324/1‑140 (MQ=255)                          ccGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTg                                                                                                       >  1:414825/1‑140 (MQ=255)                            gTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAg                                                                                                     >  1:494540/1‑140 (MQ=255)                                 cGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTc                                                                                                <  2:1139594/140‑1 (MQ=255)                                      aTCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTgggg                                                                                           <  2:271638/140‑1 (MQ=255)                                          cAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGa                                                                                       >  1:215069/1‑140 (MQ=255)                                           aaCACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGaa                                                                                      <  2:215069/140‑1 (MQ=255)                                           aaCACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGaa                                                                                      <  1:1087633/140‑1 (MQ=255)                                                  cTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTc                                                                               >  2:210955/1‑140 (MQ=255)                                                  cTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTc                                                                               >  2:467815/1‑140 (MQ=255)                                                  cTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATAGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTc                                                                               >  1:591702/1‑140 (MQ=255)                                                     cTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACCTCGTTGCCTGGCGCGCTGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGGGAGCCGTCTGGGGCCGAAAGCCTTCGAg                                                                            >  2:328022/1‑140 (MQ=255)                                                     cTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAg                                                                            >  1:215112/1‑140 (MQ=255)                                                                      cgcgTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGcttctc                                                           <  1:328022/140‑2 (MQ=255)                                                                       gcgTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTg                                                          <  2:591324/140‑1 (MQ=255)                                                                        cgTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTgc                                                         >  1:1098910/1‑140 (MQ=255)                                                                        cgTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTgc                                                         >  1:882244/1‑140 (MQ=255)                                                                          tGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTgcgc                                                       <  1:416366/140‑1 (MQ=255)                                                                             cGGGTCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCAtt                                                    <  2:1070631/140‑1 (MQ=255)                                                                             cGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCAtt                                                    <  1:553073/140‑1 (MQ=255)                                                                                 ccTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAAcc                                                <  2:985053/140‑1 (MQ=255)                                                                                            tgatgGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAAc                                     >  2:822108/1‑140 (MQ=255)                                                                                               tgGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCg                                  >  1:202548/1‑140 (MQ=255)                                                                                                  cgcAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTg                               <  2:1117943/140‑1 (MQ=255)                                                                                                  cgcAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTg                               <  2:882244/140‑1 (MQ=255)                                                                                                  cgcAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTg                               >  1:1117943/1‑140 (MQ=255)                                                                                                    cAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGa                             <  2:202548/140‑1 (MQ=255)                                                                                                    cAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGa                             <  1:822108/140‑1 (MQ=255)                                                                                                    cAAAACATCGTTGCCTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGa                             <  2:414825/140‑1 (MQ=255)                                                                                                                 ccTGGCGCGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGtt                >  1:1062093/1‑140 (MQ=255)                                                                                                                     gcgcGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCAcc            >  1:973758/1‑140 (MQ=255)                                                                                                                     gcgcGATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCAcc            <  1:467815/140‑1 (MQ=255)                                                                                                                      cgcgATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGAc                            >  1:308345/1‑123 (MQ=255)                                                                                                                      cgcgATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGAc                            <  2:308345/123‑1 (MQ=255)                                                                                                                      cgcgATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTc               <  1:840857/136‑1 (MQ=255)                                                                                                                      cgcgATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTc               >  2:840857/1‑136 (MQ=255)                                                                                                                      cgcgATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCAccc           >  2:779597/1‑140 (MQ=255)                                                                                                                        cgATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTCAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCgg         <  2:973758/140‑1 (MQ=255)                                                                                                                         gATGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGa        <  2:1062093/140‑1 (MQ=255)                                                                                                                          aTGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGaa       <  2:1098910/140‑1 (MQ=255)                                                                                                                           tGAGAACGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAg      >  1:676641/1‑140 (MQ=255)                                                                                                                               aaCGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTa  >  1:234566/1‑140 (MQ=255)                                                                                                                               aaCGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTa  <  2:215112/140‑1 (MQ=255)                                                                                                                               aaCGGCCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTa  >  1:1041461/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       GCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTA  >  NC_000913/3538837‑3539102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑