Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,746	A→T	I47F (ATC→TTC)	ykgE →	putative lactate utilization oxidoreductase YkgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,746	0	A	T	100.0%	243.2 / NA	69	I47F (ATC→TTC)	ykgE	putative lactate utilization oxidoreductase YkgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (33/36);  total (33/36)

ATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTG  >  NC_000913/321612‑321880                                                                                                                                       |                                                                                                                                       aTGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAAt                                                                                                                                   <  1:99770/140‑1 (MQ=255) aTGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAAt                                                                                                                                   <  2:1015927/140‑1 (MQ=255)  tGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATa                                                                                                                                  <  1:681979/140‑1 (MQ=255)  tGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATa                                                                                                                                  >  1:57395/1‑140 (MQ=255)    tCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGc                                                                                                                                >  1:914422/1‑140 (MQ=255)       aTTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGt                                                                                                                             <  2:1044459/140‑1 (MQ=255)                  aCCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGGTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAaaga                                                                                                                  <  1:1121985/140‑1 (MQ=255)                    cTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAaagaag                                                                                                                <  1:394253/140‑1 (MQ=255)                     tGTATTGGTGACGCCCTGAAATCAAGAATGGCACGCGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGc                                                                                                               <  2:800283/140‑1 (MQ=255)                     tGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGc                                                                                                               <  1:906182/140‑1 (MQ=255)                          tGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTc                                                                                                          >  2:148305/1‑140 (MQ=255)                               aCGCCCTGAAATCAAGAATGGCACGGGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAggg                                                                                                     >  2:306445/1‑140 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAaagaag                                                                                                                >  1:39634/1‑127 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAaagaag                                                                                                                <  1:471699/127‑1 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAaagaag                                                                                                                >  2:471699/1‑127 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAaagaag                                                                                                                <  2:39634/127‑1 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGAt                                                                                                   >  1:293887/1‑140 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGAt                                                                                                   >  1:441998/1‑140 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGAt                                                                                                   >  1:191403/1‑140 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGAt                                                                                                   >  1:882836/1‑140 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGAt                                                                                                   >  1:506748/1‑140 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGAt                                                                                                   >  1:542889/1‑140 (MQ=255)                                 gCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGAt                                                                                                   >  2:562307/1‑140 (MQ=255)                                   ccTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTc                                                                                                          >  2:123479/1‑131 (MQ=255)                                   ccTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTc                                                                                                          <  1:123479/131‑1 (MQ=255)                                             aGAATGGCACGGGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGAt                                                                                       <  1:306445/140‑1 (MQ=255)                                               aaTGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcg                                                                                     <  2:506748/140‑1 (MQ=255)                                                 tGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcgcc                                                                                   >  1:592965/1‑140 (MQ=255)                                                   gCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcgccgc                                                                                 >  1:641242/1‑140 (MQ=255)                                                    cACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcgccg                                                                                  >  1:485361/1‑138 (MQ=255)                                                    cACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATcgccg                                                                                  <  2:485361/138‑1 (MQ=255)                                                    cACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCa                                                                                >  1:742241/1‑140 (MQ=255)                                                    cACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCa                                                                                <  2:542889/140‑1 (MQ=255)                                                    cACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCa                                                                                <  2:882836/140‑1 (MQ=255)                                                       gagaCTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTg                                                                             <  2:914422/140‑1 (MQ=255)                                                       gagaCTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTg                                                                             <  2:191403/140‑1 (MQ=255)                                                       gagaCTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTg                                                                             <  1:1147675/140‑1 (MQ=255)                                                           cTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGGGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTggagg                                                                         <  1:632006/140‑1 (MQ=255)                                                            tCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTgg                                                                            >  1:475914/1‑136 (MQ=255)                                                            tCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTgg                                                                            <  2:475914/136‑1 (MQ=255)                                                              cGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATa                                                                      >  1:665530/1‑140 (MQ=255)                                                              cGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCCGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATa                                                                      >  1:157319/1‑140 (MQ=255)                                                               gTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATaa                                                                     >  1:12993/1‑140 (MQ=255)                                                                   tgctACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTg                                                                             >  2:1014550/1‑128 (MQ=255)                                                                   tgctACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTg                                                                             <  1:1014550/128‑1 (MQ=255)                                                                     ctACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTg                                                                             <  2:508730/126‑1 (MQ=255)                                                                     ctACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTg                                                                             >  1:508730/1‑126 (MQ=255)                                                                     ctACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAAcgacga                                                               >  1:715358/1‑140 (MQ=255)                                                                      tACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGAt                                                              <  2:665530/140‑1 (MQ=255)                                                                      tACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGAt                                                              <  1:769215/140‑1 (MQ=255)                                                                        cTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATcc                                                            <  2:12993/140‑1 (MQ=255)                                                                               aaCTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCattatt                                                     >  1:228191/1‑140 (MQ=255)                                                                                         tCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCggctg                                           <  1:562307/140‑1 (MQ=255)                                                                                           gcgTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCggctggc                                         <  2:715358/140‑1 (MQ=255)                                                                                              tAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAAGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGctct                                      <  1:247155/140‑1 (MQ=255)                                                                                               aaaTTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCtt                                     <  2:293887/140‑1 (MQ=255)                                                                                                      ccGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCggct                                            <  2:1087124/126‑1 (MQ=255)                                                                                                      ccGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCggct                                            >  1:1087124/1‑126 (MQ=255)                                                                                                       cGGAGAAACAGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTAt                             >  2:91539/1‑140 (MQ=255)                                                                                                                aGGGATGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTaaaa                    <  2:742241/140‑1 (MQ=255)                                                                                                                    aTGCTGCGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGtt                >  1:895918/1‑140 (MQ=255)                                                                                                                       ctgcGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTAcc             >  2:362069/1‑140 (MQ=255)                                                                                                                       ctgcGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTAcc             >  2:1134331/1‑140 (MQ=255)                                                                                                                        tgcGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTAccc            >  1:951343/1‑140 (MQ=255)                                                                                                                         gcGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCg           >  2:454876/1‑140 (MQ=255)                                                                                                                         gcGGTCAGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCg           <  1:362069/140‑1 (MQ=255)                                                                                                                               aGCCTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTAt     <  2:779698/140‑1 (MQ=255)                                                                                                                                  cTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTg  <  2:201168/140‑1 (MQ=255)                                                                                                                                  cTGCGTTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTg  <  1:1134331/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTG  >  NC_000913/321612‑321880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑