Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,614,768	T→C	F368L (TTC→CTC)	nikA →	Ni(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,614,768	0	T	C	100.0%	189.5 / NA	52	F368L (TTC→CTC)	nikA	Ni(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/28);  total (24/28)

CGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGTTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGTATCTATGCTCGTCAGCGCGACGGTCGTT  >  NC_000913/3614632‑3614900                                                                                                                                         |                                                                                                                                     cGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCa                                                                                                                                   >  1:838563/1‑140 (MQ=255) cGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCa                                                                                                                                   >  2:590341/1‑140 (MQ=255)  gCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCAt                                                                                                                                  <  1:590341/140‑1 (MQ=255)  gCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCAt                                                                                                                                  <  2:30042/140‑1 (MQ=255)     aaCCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCgg                                                                                                                               <  2:838563/140‑1 (MQ=255)     aaCCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCgg                                                                                                                               <  2:681622/140‑1 (MQ=255)           ggCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGa                                                                                                                         >  2:481932/1‑140 (MQ=255)           ggCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAACCGGTTGGACGCTGCCTGCGGGCAAAGACATGCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGa                                                                                                                         >  2:1013074/1‑140 (MQ=255)               tGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCg                                                                                                                     <  2:1072529/140‑1 (MQ=255)                gAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGt                                                                                                                    >  1:565810/1‑140 (MQ=255)                gAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGt                                                                                                                    >  1:1088120/1‑140 (MQ=255)                 aaaCCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGtt                                                                                                                   >  2:936163/1‑140 (MQ=255)                            gTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGa                                                                                                        <  2:1101361/140‑1 (MQ=255)                             tACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGATATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGAt                                                                                                       >  2:1021549/1‑140 (MQ=255)                                  tCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCgg                                                                                                  >  1:324589/1‑140 (MQ=255)                                    cGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGaa                                                                                                <  2:324589/140‑1 (MQ=255)                                       aaaaaGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATc                                                                                             >  2:639186/1‑140 (MQ=255)                                          aagcgaaagcgTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCAtt                                                                                          <  1:220379/140‑1 (MQ=255)                                          aagcgaaagcgTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCAtt                                                                                          <  2:1088120/140‑1 (MQ=255)                                          aagcgaaagcgTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCAtt                                                                                          <  1:83368/140‑1 (MQ=255)                                          aagcgaaagcgTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCAtt                                                                                          <  2:565810/140‑1 (MQ=255)                                                              aaaGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGAt                                                                      <  2:738479/140‑1 (MQ=255)                                                               aaGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGAtt                                                                     >  2:499573/1‑140 (MQ=255)                                                               aaGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCAATCAGGCTGata                                                                                >  1:634674/1‑129 (MQ=255)                                                               aaGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCAATCAGGCTGata                                                                                <  2:634674/129‑1 (MQ=255)                                                                aGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATgcgc                                                                           >  2:443353/1‑133 (MQ=255)                                                                aGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATgcgc                                                                           <  1:443353/133‑1 (MQ=255)                                                                 gCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTgg                                                                   <  2:936405/140‑1 (MQ=255)                                                                                    cGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGAtt                                                >  2:981991/1‑140 (MQ=255)                                                                                        cAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGAt                                                 <  1:554840/135‑1 (MQ=255)                                                                                        cAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGAt                                                 >  2:554840/1‑135 (MQ=255)                                                                                        cAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCg                                            >  2:680090/1‑140 (MQ=255)                                                                                          aaGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCgaa                                          <  2:561439/140‑1 (MQ=255)                                                                                                 ccGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAagaga                                   >  2:958032/1‑140 (MQ=255)                                                                                                 ccGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAagaga                                   >  1:1105876/1‑140 (MQ=255)                                                                                                 ccGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAagaga                                   >  1:489459/1‑140 (MQ=255)                                                                                                  cgcgAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAagagag                                  <  2:1105876/140‑1 (MQ=255)                                                                                                  cgcgAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAagagag                                  <  2:707512/140‑1 (MQ=255)                                                                                                  cgcgAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAagagag                                  <  2:489459/140‑1 (MQ=255)                                                                                                    cgAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCa                                <  1:936163/140‑1 (MQ=255)                                                                                                    cgAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCa                                <  1:481932/140‑1 (MQ=255)                                                                                                    cgAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCa                                <  1:958032/140‑1 (MQ=255)                                                                                                    cgAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCa                                <  1:1013074/140‑1 (MQ=255)                                                                                                     gagaAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAg                               >  2:380825/1‑140 (MQ=255)                                                                                                      agaAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGt                              >  1:268051/1‑140 (MQ=255)                                                                                                       gaAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGaagaag                                      <  1:709297/131‑1 (MQ=255)                                                                                                       gaAAAATGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGaagaag                                      >  2:709297/1‑131 (MQ=255)                                                                                                        aaaaaTGGTCAGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGTAt                            <  2:141101/140‑1 (MQ=255)                                                                                                                  aGCCGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGTATCTATGCTCGt                  >  1:219632/1‑140 (MQ=255)                                                                                                                    ccGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCa                                >  2:446782/1‑124 (MQ=255)                                                                                                                    ccGCTGCGCATTGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCa                                <  1:446782/124‑1 (MQ=255)                                                                                                                              ttGAACTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGTATCTATGCTCGTCAGCGCGACGGt      <  1:680090/140‑1 (MQ=255)                                                                                                                                  aCTTTCGCTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGTATCTATGCTCGTCAGCGCGACGGTCGtt  <  1:1021549/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGTTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGTATCTATGCTCGTCAGCGCGACGGTCGTT  >  NC_000913/3614632‑3614900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑