Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,936,864	A→G	N196S (AAC→AGC)	rbsB →	ribose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,936,864	0	A	G	100.0%	265.4 / NA	72	N196S (AAC→AGC)	rbsB	ribose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/36);  total (36/36)

AGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGT  >  NC_000913/3936735‑3937000                                                                                                                                  |                                                                                                                                         aGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACCAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAg                                                                                                                                <  1:1015316/140‑1 (MQ=255) aGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAg                                                                                                                                <  2:1049679/140‑1 (MQ=255)   cTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGaa                                                                                                                              >  2:955950/1‑140 (MQ=255)   cTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGaa                                                                                                                              >  2:793229/1‑140 (MQ=255)                cTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCt                                                                                                                 >  1:378353/1‑140 (MQ=255)                 tGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTc                                                                                                                <  2:90366/140‑1 (MQ=255)                      cATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCg                                                                                                           <  1:793229/140‑1 (MQ=255)                        tCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGa                                                                                                         <  1:805816/140‑1 (MQ=255)                        tCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGa                                                                                                         >  1:999750/1‑140 (MQ=255)                        tCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGa                                                                                                         >  1:189474/1‑140 (MQ=255)                        tCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGa                                                                                                         >  2:832248/1‑140 (MQ=255)                             aGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGtt                                                                                                     >  2:580715/1‑139 (MQ=255)                             aGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGtt                                                                                                     <  1:580715/139‑1 (MQ=255)                              gCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCa                                                                                                   >  2:951198/1‑140 (MQ=255)                                 cGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGc                                                                                                <  2:780678/140‑1 (MQ=255)                                   tGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTg                                                                                              >  2:467746/1‑140 (MQ=255)                                   tGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTg                                                                                              >  2:272521/1‑140 (MQ=255)                                          ggCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGtt                                                                                                     <  2:135501/126‑1 (MQ=255)                                          ggCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGtt                                                                                                     >  1:135501/1‑126 (MQ=255)                                          ggCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgc                                                                                       >  1:1067558/1‑140 (MQ=255)                                           gCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                      <  1:808584/140‑1 (MQ=255)                                           gCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                      <  2:378353/140‑1 (MQ=255)                                           gCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                      <  2:1067558/140‑1 (MQ=255)                                           gCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                      <  2:1020506/140‑1 (MQ=255)                                             gAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCa                                                                                    >  2:168060/1‑140 (MQ=255)                                             gAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGAGCa                                                                                    <  1:272521/140‑1 (MQ=255)                                             gAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAAAGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCa                                                                                    <  1:1009718/140‑1 (MQ=255)                                             gAAGGCTTCCAGCAGGCCATTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCa                                                                                    <  1:425619/140‑1 (MQ=255)                                               aGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                      >  2:993963/1‑136 (MQ=255)                                               aGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                      <  1:993963/136‑1 (MQ=255)                                                ggCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                      <  2:1040403/135‑1 (MQ=255)                                                ggCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTcgcg                                                                                      >  1:1040403/1‑135 (MQ=255)                                                        gcagGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGaaa                                                                         <  1:951198/140‑1 (MQ=255)                                                           ggCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATgg                                                                      >  1:629616/1‑140 (MQ=255)                                                             ccGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGgcg                                                                    >  1:374427/1‑140 (MQ=255)                                                              cGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAatga                                                                              <  2:751909/129‑1 (MQ=255)                                                              cGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAatga                                                                              >  1:751909/1‑129 (MQ=255)                                                              cGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGgcgc                                                                   >  2:1074050/1‑140 (MQ=255)                                                                ttGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTg                                                                 <  2:374427/140‑1 (MQ=255)                                                                      ctCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcg                                                           >  1:646719/1‑140 (MQ=255)                                                                        cacaAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCt                                                         <  1:955950/140‑1 (MQ=255)                                                                          caAGTTTAATGTTCTTGTCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgc                                                       >  1:344337/1‑140 (MQ=255)                                                                            aGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgc                                                     <  1:168060/140‑1 (MQ=255)                                                                              tttAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgcgc                                                   <  2:646719/140‑1 (MQ=255)                                                                                tAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCAc                                                 >  1:476121/1‑140 (MQ=255)                                                                                    gTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCa                                             <  2:189474/140‑1 (MQ=255)                                                                                       cTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAc                                          >  1:442791/1‑140 (MQ=255)                                                                                       cTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAc                                          >  1:833979/1‑140 (MQ=255)                                                                                        ttGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACt                                         >  1:962289/1‑140 (MQ=255)                                                                                         tGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCa                                             >  1:867161/1‑135 (MQ=255)                                                                                         tGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCa                                             <  2:867161/135‑1 (MQ=255)                                                                                             agccagccagcAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCgg                                    <  1:1074050/140‑1 (MQ=255)                                                                                                  gccagcAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAt                               >  2:247746/1‑140 (MQ=255)                                                                                                  gccagcAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAAt                               >  1:1081253/1‑140 (MQ=255)                                                                                                   ccagcAGATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATc                              <  1:467746/140‑1 (MQ=255)                                                                                                     agcagATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCgg                            <  2:476121/140‑1 (MQ=255)                                                                                                     agcagATTTTGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCgg                            <  1:501267/140‑1 (MQ=255)                                                                                                             ttGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATg                    >  2:492327/1‑140 (MQ=255)                                                                                                             ttGATCGCATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATg                    >  1:807758/1‑140 (MQ=255)                                                                                                                    cATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGtcgtcg             >  1:506047/1‑140 (MQ=255)                                                                                                                    cATTAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGtcgtcg             >  1:7233/1‑140 (MQ=255)                                                                                                                      ttAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGa           <  1:832248/140‑1 (MQ=255)                                                                                                                      ttAAAGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGa           >  2:389669/1‑140 (MQ=255)                                                                                                                        aaaGGTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGAtt         <  2:794585/140‑1 (MQ=255)                                                                                                                           ggTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGa      <  1:492327/140‑1 (MQ=255)                                                                                                                           ggTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGa      <  1:247300/140‑1 (MQ=255)                                                                                                                           ggTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGa      <  1:912469/140‑1 (MQ=255)                                                                                                                           ggTTTGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGa      <  2:994651/140‑1 (MQ=255)                                                                                                                               tGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTTGGATTTGACgga  >  1:12724/1‑139 (MQ=255)                                                                                                                               tGAGCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGt  >  2:499002/1‑140 (MQ=255)                                                                                                                               tGAGCGTAATGCAGAACCTGTTGACCACTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGAtt         <  1:496949/133‑1 (MQ=255)                                                                                                                               tGAGCGTAATGCAGAACCTGTTGACCACTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGAtt         >  2:496949/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                                         AGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCGGATTTGACGGT  >  NC_000913/3936735‑3937000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑