Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,202,974	T→C	I350T (ATT→ACT)	zraS →	sensory histidine kinase ZraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,202,974	0	T	C	100.0%	352.1 / NA	95	I350T (ATT→ACT)	zraS	sensory histidine kinase ZraS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/49);  total (46/49)

GGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAATTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCGGGCGTGAAAATCAGCGTTACCGAC  >  NC_000913/4202837‑4203110                                                                                                                                          |                                                                                                                                         ggTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTc                                                                                                                                        >  1:1157712/1‑140 (MQ=255)  gTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTTATTTCAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGATACGCTTTACCGCCAACGACACATTACCGGAAACTCa                                                                                                                                       >  2:1037282/1‑140 (MQ=255)   ttAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATTTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGGTGCAAACCGCCGGGAGATCCAGTTACGCTTTTCCGCCAACGACACATTACCGGAAACTCAg                                                                                                                                      >  2:551187/1‑140 (MQ=255)    tAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAgg                                                                                                                                     <  1:915099/140‑1 (MQ=255)    tAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAgg                                                                                                                                     <  2:3200/140‑1 (MQ=255)        ccAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGa                                                                                                                                 <  1:80626/140‑1 (MQ=255)        ccAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGa                                                                                                                                 <  2:6022/140‑1 (MQ=255)         cAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGAc                                                                                                                                <  2:157500/140‑1 (MQ=255)            cGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCg                                                                                                                             >  2:243752/1‑140 (MQ=255)            cGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCg                                                                                                                             >  1:193581/1‑140 (MQ=255)            cGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCg                                                                                                                             >  1:447740/1‑140 (MQ=255)            cGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCg                                                                                                                             >  2:419203/1‑140 (MQ=255)            cGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCg                                                                                                                             >  1:943600/1‑140 (MQ=255)            cGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCg                                                                                                                             >  2:86040/1‑140 (MQ=255)              cATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGa                                                                                                                           <  2:452120/140‑1 (MQ=255)                  tGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAgg                                                                                                                       <  1:905601/140‑1 (MQ=255)                  tGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAgg                                                                                                                       <  1:1028234/140‑1 (MQ=255)                   ggCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGc                                                                                                                      <  1:930946/140‑1 (MQ=255)                        tGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGa                                                                                                                           >  1:28627/1‑130 (MQ=255)                        tGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGa                                                                                                                           <  2:28627/130‑1 (MQ=255)                         gCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTc                                                                                                                <  1:86040/140‑1 (MQ=255)                         gCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTc                                                                                                                <  2:1157712/140‑1 (MQ=255)                         gCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTc                                                                                                                <  2:396370/140‑1 (MQ=255)                         gCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTc                                                                                                                <  1:419203/140‑1 (MQ=255)                              cGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTc                                                                                                           <  1:234013/140‑1 (MQ=255)                                     tctcAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGa                                                                                                    <  1:243752/140‑1 (MQ=255)                                         aaCACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAgg                                                                                                             >  1:1143134/1‑127 (MQ=255)                                         aaCACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAgg                                                                                                             <  2:1143134/127‑1 (MQ=255)                                         aaCACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAgg                                                                                                             <  2:842857/127‑1 (MQ=255)                                         aaCACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAgg                                                                                                             >  1:842857/1‑127 (MQ=255)                                          acacGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAAtctc                                                                                               <  2:1120569/140‑1 (MQ=255)                                          acacGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAAtctc                                                                                               <  2:708515/140‑1 (MQ=255)                                          acacGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAAtctc                                                                                               <  2:943600/140‑1 (MQ=255)                                          acacGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAAtctc                                                                                               <  2:193581/140‑1 (MQ=255)                                          acacGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAAtctc                                                                                               <  2:963856/140‑1 (MQ=255)                                           cacGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATctct                                                                                              >  2:344065/1‑140 (MQ=255)                                             cGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTAt                                                                                            >  1:1061494/1‑140 (MQ=255)                                              gCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGt                                                                                                       >  2:166636/1‑128 (MQ=255)                                              gCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGt                                                                                                       <  1:166636/128‑1 (MQ=255)                                              gCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTAtc                                                                                           >  2:711998/1‑140 (MQ=255)                                               cTGATTAACCAATCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTcc                                                                                                          >  2:209292/1‑124 (MQ=255)                                               cTGATTAACCAATCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTcc                                                                                                          <  1:209292/124‑1 (MQ=255)                                                tGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACTACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTAtctc                                                                                         >  2:1140597/1‑140 (MQ=255)                                                tGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGa                                                                                                    <  1:912884/129‑1 (MQ=255)                                                tGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGa                                                                                                    >  2:912884/1‑129 (MQ=255)                                                tGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTAtctc                                                                                         >  1:1064419/1‑140 (MQ=255)                                                tGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTAtctc                                                                                         >  1:394219/1‑140 (MQ=255)                                                    tAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATg                                                                                     <  1:711998/140‑1 (MQ=255)                                                      aCCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCt                                                                                   <  1:4567/140‑1 (MQ=255)                                                         aCTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACTACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTAtt                                                                                <  1:1140597/140‑1 (MQ=255)                                                          cTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAAtct                                                                                                >  2:1010884/1‑123 (MQ=255)                                                          cTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAAtct                                                                                                <  1:1010884/123‑1 (MQ=255)                                                           tCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCGGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCa                                                                              <  2:1078762/140‑1 (MQ=255)                                                           tCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCa                                                                              <  2:402675/140‑1 (MQ=255)                                                             aTTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAgg                                                                            <  1:174576/140‑1 (MQ=255)                                                              ttACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGc                                                                           <  2:250840/140‑1 (MQ=255)                                                               tACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCg                                                                          >  1:1069678/1‑140 (MQ=255)                                                                    cTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTgg                                                                     >  1:82544/1‑140 (MQ=255)                                                                    cTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCACTATCTCAATGCTATTCAGGCGATTgg                                                                     >  2:830114/1‑140 (MQ=255)                                                                     tGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGt                                                                    >  2:791849/1‑140 (MQ=255)                                                                      ggTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTc                                                                   >  1:413580/1‑140 (MQ=255)                                                                       gTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCa                                                                  >  2:1111501/1‑140 (MQ=255)                                                                       gTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCa                                                                  >  2:659569/1‑140 (MQ=255)                                                                        tAAGTCAGGATGCAAACATCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGa                                                                         >  1:683221/1‑132 (MQ=255)                                                                        tAAGTCAGGATGCAAACATCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGa                                                                         <  2:683221/132‑1 (MQ=255)                                                                         aaGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCCACGACACATTACCGGAAACTCAGTCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGc                                                                >  2:874022/1‑140 (MQ=255)                                                                         aaGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGc                                                                <  1:1037282/140‑1 (MQ=255)                                                                            tCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGc                                                                >  2:1106541/1‑137 (MQ=255)                                                                            tCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGc                                                                <  1:1106541/137‑1 (MQ=255)                                                                            tCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATg                                                             >  2:420302/1‑140 (MQ=255)                                                                              aGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGc                                                           >  1:408237/1‑140 (MQ=255)                                                                              aGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGc                                                           >  2:928724/1‑140 (MQ=255)                                                                               ggATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCg                                                          <  2:447740/140‑1 (MQ=255)                                                                                gATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTgg                                                                     >  2:592996/1‑128 (MQ=255)                                                                                gATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTgg                                                                     <  1:592996/128‑1 (MQ=255)                                                                                 aTGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGc                                                           >  2:1080322/1‑137 (MQ=255)                                                                                 aTGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGc                                                           <  1:1080322/137‑1 (MQ=255)                                                                                 aTGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTg                                                        >  1:1116551/1‑140 (MQ=255)                                                                                  tGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGa                                                       <  2:931435/140‑1 (MQ=255)                                                                                          gCCGGGGGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGCATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTg                                               >  2:1029697/1‑140 (MQ=255)                                                                                           ccGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGa                                              >  1:163428/1‑140 (MQ=255)                                                                                              ggAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACgg                                           <  2:1069678/140‑1 (MQ=255)                                                                                              ggAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACgg                                           >  2:1081068/1‑140 (MQ=255)                                                                                                    ccAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCg                                     <  2:827267/140‑1 (MQ=255)                                                                                                     cAGTTACGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGa                                    >  2:342785/1‑140 (MQ=255)                                                                                                          aCGCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAgcg                               >  1:490090/1‑140 (MQ=255)                                                                                                            gCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAgcggc                             >  1:584579/1‑140 (MQ=255)                                                                                                            gCTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAgcggc                             <  1:830114/140‑1 (MQ=255)                                                                                                             cTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAgcggcg                            <  1:252285/140‑1 (MQ=255)                                                                                                             cTTTACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAgcggcg                            >  1:769424/1‑140 (MQ=255)                                                                                                                tACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACgg                                           <  1:866419/122‑1 (MQ=255)                                                                                                                tACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACgg                                           >  2:866419/1‑122 (MQ=255)                                                                                                                tACCGCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCgg                         >  1:532632/1‑140 (MQ=255)                                                                                                                    gCCAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCGGGCGt                     <  2:413580/140‑1 (MQ=255)                                                                                                                     ccAACGACACATTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCGGGCGTg                    >  2:570495/1‑140 (MQ=255)                                                                                                                            cacaTTACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCGGGCGTGAAAATCa             <  1:342785/140‑1 (MQ=255)                                                                                                                                 tACCGGAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCGGGCGTGAAAATCAGCGtt        >  2:119131/1‑140 (MQ=255)                                                                                                                                  aCCGGAAACTCAAGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCGGGCGTGAAAATCAGCGTTa       >  2:183774/1‑140 (MQ=255)                                                                                                                                      gAAACTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCGGGCGTGAAAATCAGCGTTACCGa   >  2:764505/1‑140 (MQ=255)                                                                                                                                       aaaCTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCAAAAGCGGCGCGGGCGTGAAAATCAGCGTTACCGAc  <  1:874022/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTGGTAAGTCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAATTCAGGCCGACCCGGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGACGGCCAGCGAAAGCGGCGCGGGCGTGAAAATCAGCGTTACCGAC  >  NC_000913/4202837‑4203110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑