Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,264,342	A→G	Q10R (CAG→CGG)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,264,342	0	A	G	100.0%	328.5 / NA	91	Q10R (CAG→CGG)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (56/35);  total (56/35)

GAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAG  >  NC_000913/4264210‑4264472                                                                                                                                     |                                                                                                                                   gAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCt                                                                                                                             >  2:1126634/1‑140 (MQ=255)                        tgtCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCcaca                                                                                                      >  2:1158282/1‑140 (MQ=255)                        tgtCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCcaca                                                                                                      <  1:563752/140‑1 (MQ=255)                           cctccAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGt                                                                                                   >  1:707236/1‑140 (MQ=255)                             tccAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACaa                                                                                                     <  1:423027/136‑1 (MQ=255)                             tccAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACaa                                                                                                     >  2:423027/1‑136 (MQ=255)                             tccAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTg                                                                                                 >  2:704353/1‑140 (MQ=255)                              ccAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGc                                                                                                <  1:704353/140‑1 (MQ=255)                                  aaCGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCggg                                                                                            >  2:326793/1‑140 (MQ=255)                                    cGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCt                                                                                          >  2:1034510/1‑140 (MQ=255)                                       aTCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCggg                                                                                            >  2:1020873/1‑135 (MQ=255)                                       aTCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCggg                                                                                            <  1:1020873/135‑1 (MQ=255)                                       aTCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGaa                                                                                       >  1:466388/1‑140 (MQ=255)                                         cGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAg                                                                                     >  1:1088341/1‑140 (MQ=255)                                          gTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGt                                                                                    <  1:1158282/140‑1 (MQ=255)                                            cAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGc                                                                                  >  1:1115793/1‑140 (MQ=255)                                              gggTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCt                                                                                >  1:531352/1‑140 (MQ=255)                                                   tGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCa                                                                           <  1:326793/140‑1 (MQ=255)                                                   tGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCa                                                                           >  2:1026592/1‑140 (MQ=255)                                                    gCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCAc                                                                          >  2:909493/1‑140 (MQ=255)                                                     cTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACt                                                                         >  1:764728/1‑140 (MQ=255)                                                     cTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACt                                                                         >  2:60351/1‑140 (MQ=255)                                                     cTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACt                                                                         >  1:435213/1‑140 (MQ=255)                                                         atatGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACtcgat                                                                     <  2:1088341/140‑1 (MQ=255)                                                         atatGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACtcgat                                                                     <  1:677660/140‑1 (MQ=255)                                                          tatGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACtcgatc                                                                    >  1:52579/1‑140 (MQ=255)                                                          tatGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACtcgatc                                                                    >  1:695858/1‑140 (MQ=255)                                                          tatGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACtcgatc                                                                    >  1:732811/1‑140 (MQ=255)                                                           atGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACtcgatcg                                                                   <  1:871458/140‑1 (MQ=255)                                                               tAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGc                                                               >  2:1143649/1‑140 (MQ=255)                                                               tAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGc                                                               >  2:198843/1‑140 (MQ=255)                                                               tAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGc                                                               >  1:814422/1‑140 (MQ=255)                                                               tAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGc                                                               >  2:491615/1‑140 (MQ=255)                                                               tAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGc                                                               >  2:903564/1‑140 (MQ=255)                                                                  aGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCa                                                                           >  1:982394/1‑125 (MQ=255)                                                                  aGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCa                                                                           <  2:982394/125‑1 (MQ=255)                                                                  aGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGa                                                            >  2:101885/1‑140 (MQ=255)                                                                  aGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGa                                                            <  1:980823/140‑1 (MQ=255)                                                                  aGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGa                                                            <  1:1143649/140‑1 (MQ=255)                                                                  aGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGa                                                            <  1:1026592/140‑1 (MQ=255)                                                                       cGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                       <  2:764728/140‑1 (MQ=255)                                                                       cGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                       <  2:695858/140‑1 (MQ=255)                                                                       cGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                       >  1:726266/1‑140 (MQ=255)                                                                       cGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                       <  2:52579/140‑1 (MQ=255)                                                                             aTTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                       >  1:734777/1‑134 (MQ=255)                                                                             aTTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGt                                                       <  2:734777/134‑1 (MQ=255)                                                                                 tttATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTggg                                             <  2:732811/140‑1 (MQ=255)                                                                                 tttATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTggg                                             <  2:1070754/140‑1 (MQ=255)                                                                                   tATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCg                                           >  2:35575/1‑140 (MQ=255)                                                                                         ggTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTgg                                              >  1:84395/1‑131 (MQ=255)                                                                                         ggTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTgg                                              <  2:84395/131‑1 (MQ=255)                                                                                         ggTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTaa                                     >  1:994660/1‑140 (MQ=255)                                                                                         ggTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTaa                                     >  1:391064/1‑140 (MQ=255)                                                                                         ggTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTaa                                     >  1:380075/1‑140 (MQ=255)                                                                                         ggTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTaa                                     >  1:1015053/1‑140 (MQ=255)                                                                                         ggTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTaa                                     >  2:1159795/1‑140 (MQ=255)                                                                                         ggTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTaa                                     >  1:876689/1‑140 (MQ=255)                                                                                          gTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAAt                                    >  1:13818/1‑140 (MQ=255)                                                                                           tAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATg                                   <  1:491615/140‑1 (MQ=255)                                                                                              cTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTa                                >  1:459233/1‑140 (MQ=255)                                                                                                   ttCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaa                           >  2:650531/1‑140 (MQ=255)                                                                                                   ttCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaa                           >  2:618229/1‑140 (MQ=255)                                                                                                    tCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaac                          <  2:876689/140‑1 (MQ=255)                                                                                                    tCACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaac                          <  2:814422/140‑1 (MQ=255)                                                                                                     cACTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaacg                         <  2:385449/140‑1 (MQ=255)                                                                                                       cTATGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaacgaa                       <  1:60351/140‑1 (MQ=255)                                                                                                         aTGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaacgaacg                     >  2:305912/1‑140 (MQ=255)                                                                                                         aTGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaacgaacg                     >  2:950387/1‑140 (MQ=255)                                                                                                         aTGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaacgaacg                     <  2:13818/140‑1 (MQ=255)                                                                                                         aTGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATaacgaacg                     >  2:720578/1‑140 (MQ=255)                                                                                                          tGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGc                    <  2:391064/140‑1 (MQ=255)                                                                                                          tGGCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGc                    <  1:234193/140‑1 (MQ=255)                                                                                                           ggCAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCt                   >  1:145698/1‑140 (MQ=255)                                                                                                             cAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTgg                 >  1:839878/1‑140 (MQ=255)                                                                                                             cAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTgg                 >  1:406333/1‑140 (MQ=255)                                                                                                             cAGGAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACACTgg                 >  1:365817/1‑140 (MQ=255)                                                                                                               ggAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGga               >  2:739323/1‑140 (MQ=255)                                                                                                                gAAATAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGgat              >  1:471165/1‑140 (MQ=255)                                                                                                                 aaaTAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGgatg             <  2:380075/140‑1 (MQ=255)                                                                                                                  aaTAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTgg                 >  2:898714/1‑135 (MQ=255)                                                                                                                  aaTAAACCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTgg                 <  1:898714/135‑1 (MQ=255)                                                                                                                       aCCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAg       >  2:795108/1‑140 (MQ=255)                                                                                                                       aCCCTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAg       <  1:101885/140‑1 (MQ=255)                                                                                                                         ccTTCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGcc     <  1:1159795/140‑1 (MQ=255)                                                                                                                           ttCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGa   >  2:724411/1‑140 (MQ=255)                                                                                                                           ttCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGa   >  2:787245/1‑140 (MQ=255)                                                                                                                           ttCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGa   <  1:787245/140‑1 (MQ=255)                                                                                                                            tCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAg  >  1:1140056/1‑140 (MQ=255)                                                                                                                            tCAACAAACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAg  <  1:1126634/140‑1 (MQ=255)                                                                                                                             caacaaACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTgg                 <  2:31702/124‑1 (MQ=255)                                                                                                                             caacaaACGGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTgg                 >  1:31702/1‑124 (MQ=255)                                                                                                                                     |                                                                                                                                   GAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTAGATAACGAACGCTGGGATGATGTAGCCGAG  >  NC_000913/4264210‑4264472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑