BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,289,528	A→G	D95G (GAT→GGT)	nrfB →	periplasmic nitrite reductase penta‑heme c‑type cytochrome

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,289,528	0	A	G	100.0%	381.4 / NA	106	D95G (GAT→GGT)	nrfB	periplasmic nitrite reductase penta‑heme c‑type cytochrome
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (51/55); total (51/55)

Only 100 of 110 total aligned reads displayed.

GGACTGTCATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAG  >  NC_000913/4289394‑4289663

ggACTGTCATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGa                                                                                                                                    <  1:833921/140‑1 (MQ=255)

      tCATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCt                                                                                                                              >  2:950261/1‑140 (MQ=255)

      tCATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCt                                                                                                                              >  1:1085384/1‑140 (MQ=255)

      tCATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCt                                                                                                                              >  2:24425/1‑140 (MQ=255)

       cATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCtt                                                                                                                             >  1:1024470/1‑140 (MQ=255)

       cATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCtt                                                                                                                             >  2:649413/1‑140 (MQ=255)

       cATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCtt                                                                                                                             >  2:50050/1‑140 (MQ=255)

          aaaCCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTaa                                                                                                                          <  1:771362/140‑1 (MQ=255)

            aCCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACg                                                                                                                        <  2:28989/140‑1 (MQ=255)

             ccAGATACCGAAGTTATGCATGAAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCCCGCCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGTTTTAACGa                                                                                                                       <  2:297364/140‑1 (MQ=255)

              cAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAg                                                                                                                      <  1:24425/140‑1 (MQ=255)

                gATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGcc                                                                                                                    >  2:861732/1‑140 (MQ=255)

                gATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGcc                                                                                                                    <  1:950261/140‑1 (MQ=255)

                gATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGcc                                                                                                                    >  1:80028/1‑140 (MQ=255)

                 aTACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCg                                                                                                                   <  2:993927/140‑1 (MQ=255)

                    ccGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACAAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATg                                                                                                                <  1:1132992/140‑1 (MQ=255)

                     cGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGt                                                                                                               >  1:483290/1‑140 (MQ=255)

                       aaGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTAc                                                                                                             >  1:964330/1‑140 (MQ=255)

                       aaGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTAc                                                                                                             >  1:588393/1‑140 (MQ=255)

                        aGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAg                                                                                                                      <  2:129313/130‑1 (MQ=255)

                        aGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAg                                                                                                                      >  1:129313/1‑130 (MQ=255)

                         ggTATGCTTAGAAATCATGTTACCGTTATCAGCCCGAATAACAAACTGCCGGTCACCTGCACCACCGGCCACGGCCAGCCATCCCCGCAACACCGGGAAGGGGTAAACGGTGTGATGCGCTTTAACGAGCCGATGTACaa                                                                                                           <  2:499734/140‑1 (MQ=255)

                           tATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                                                         >  1:1001509/1‑140 (MQ=255)

                           tATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                                                         >  2:252676/1‑140 (MQ=255)

                            aTGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGt                                                                                                        >  2:404769/1‑140 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAg                                                                                                                      <  1:1528/125‑1 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAg                                                                                                                      >  2:1528/1‑125 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACaa                                                                                                           <  1:588573/136‑1 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACaa                                                                                                           >  2:588573/1‑136 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                                                         <  2:149776/138‑1 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                                                         <  2:1077249/138‑1 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                                                         >  1:149776/1‑138 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAgg                                                                                                         >  1:1077249/1‑138 (MQ=255)

                             tGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGtt                                                                                                       >  1:499734/1‑140 (MQ=255)

                              gCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTg                                                                                                      <  2:82794/140‑1 (MQ=255)

                              gCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTg                                                                                                      <  2:893401/140‑1 (MQ=255)

                                aTGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGga                                                                                                    <  2:80028/140‑1 (MQ=255)

                                   gAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGagag                                                                                                 <  1:234465/140‑1 (MQ=255)

                                   gAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGagag                                                                                                 <  1:5852/140‑1 (MQ=255)

                                    aaaaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGc                                                                                                <  1:170113/140‑1 (MQ=255)

                                    aaaaCATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGc                                                                                                >  1:736493/1‑140 (MQ=255)

                                        cATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGaa                                                                                            <  1:404769/140‑1 (MQ=255)

                                           gCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGaa                                                                                            <  1:216267/137‑1 (MQ=255)

                                           gCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGaa                                                                                            >  2:216267/1‑137 (MQ=255)

                                           gCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAg                                                                                         >  2:854790/1‑140 (MQ=255)

                                           gCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAg                                                                                         <  1:649413/140‑1 (MQ=255)

                                           gCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAg                                                                                         >  1:979037/1‑140 (MQ=255)

                                            cTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGc                                                                                        <  1:861732/140‑1 (MQ=255)

                                            cTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGc                                                                                        >  2:1162489/1‑140 (MQ=255)

                                             ttCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCg                                                                                       <  1:1162489/140‑1 (MQ=255)

                                             ttCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCg                                                                                       <  1:52635/140‑1 (MQ=255)

                                             ttCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCg                                                                                       <  1:623044/140‑1 (MQ=255)

                                               ccGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTc                                                                                     <  2:588393/140‑1 (MQ=255)

                                                    aTCAACCCGAATAACAACCTGCCGGTCACTTGCACCAACTGTCACGGCAAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGAGGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTAt                                                                                <  2:979037/140‑1 (MQ=255)

                                                      cAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTAt                                                                                <  2:1025983/138‑1 (MQ=255)

                                                      cAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTAt                                                                                >  1:1025983/1‑138 (MQ=255)

                                                       aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTc                                                                             >  2:123114/1‑140 (MQ=255)

                                                       aaCCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTc                                                                             <  1:20535/140‑1 (MQ=255)

                                                         cccGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCt                                                                           >  1:142742/1‑140 (MQ=255)

                                                         cccGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCt                                                                           >  1:150421/1‑140 (MQ=255)

                                                             aaTAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTc                                                                                     <  1:66503/126‑1 (MQ=255)

                                                             aaTAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTc                                                                                     >  2:66503/1‑126 (MQ=255)

                                                               tAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATc                                                                     <  2:419923/140‑1 (MQ=255)

                                                               aaaCAAACTGCCGGTCCCCTGCCCCAACTGTCCCGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATc                                                                     <  2:1085384/139‑1 (MQ=255)

                                                                   aaaCTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGcc                                                                 >  1:373901/1‑140 (MQ=255)

                                                                     aCTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCgg                                                               <  2:115111/140‑1 (MQ=255)

                                                                       tGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGc                                                                  <  1:305867/135‑1 (MQ=255)

                                                                       tGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGc                                                                  >  2:305867/1‑135 (MQ=255)

                                                                       tGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGaa                                                             <  2:142742/140‑1 (MQ=255)

                                                                       tGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGaa                                                             <  2:373901/140‑1 (MQ=255)

                                                                            gTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGtt                                                        >  1:997741/1‑140 (MQ=255)

                                                                            gTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGtt                                                        >  1:990572/1‑140 (MQ=255)

                                                                                 cTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCaaa                                                   >  2:635731/1‑140 (MQ=255)

                                                                                 cTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCaaa                                                   >  2:141643/1‑140 (MQ=255)

                                                                                   gCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCaaaaa                                                 >  2:1091595/1‑140 (MQ=255)

                                                                                      ccAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCg                                              <  2:964330/140‑1 (MQ=255)

                                                                                        aaCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGtt                                            >  1:925262/1‑140 (MQ=255)

                                                                                        aaCTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGtt                                            <  1:141643/140‑1 (MQ=255)

                                                                                           tGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTg                                         >  2:689970/1‑140 (MQ=255)

                                                                                            gTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTgg                                        <  1:854790/140‑1 (MQ=255)

                                                                                              cACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGcc                                      >  2:58534/1‑140 (MQ=255)

                                                                                                 ggCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCa                                   <  1:1091595/140‑1 (MQ=255)

                                                                                                   ccagccaTCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACg                                 >  2:168795/1‑140 (MQ=255)

                                                                                                    cagccaTCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGa                                >  1:927274/1‑140 (MQ=255)

                                                                                                     agcccTCCCCCCAACACCGCGAAGGGGGGAAAGGTGTGATGCGCTTTACCGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTTCCGGAACAGGTGCAAAAAGCATTCTGGCCGCACGAt                               <  2:144797/140‑1 (MQ=255)

                                                                                                       ccaTCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGt                             >  2:82990/1‑140 (MQ=255)

                                                                                                        caTCACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTc                            <  1:689970/140‑1 (MQ=255)

                                                                                                           cACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCAc                         <  1:58534/140‑1 (MQ=255)

                                                                                                           cACCGCAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCAc                         <  2:150421/140‑1 (MQ=255)

                                                                                                                cAACACCGCGAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAAc                    >  1:1054381/1‑140 (MQ=255)

                                                                                                                        cgAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTgg            >  1:989984/1‑140 (MQ=255)

                                                                                                                        cgAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTgg            >  2:487807/1‑140 (MQ=255)

                                                                                                                        cgAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTgg            >  2:1034090/1‑140 (MQ=255)

                                                                                                                        cgAAGGGGTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTgg            >  1:1142083/1‑140 (MQ=255)

                                                                                                                            ggggTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTTATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTg        <  1:123114/140‑1 (MQ=255)

                                                                                                                              ggTGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTgcg      <  1:252676/140‑1 (MQ=255)

                                                                                                                                tGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGcc    >  1:183475/1‑140 (MQ=255)

                                                                                                                                tGAAAGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGcc    <  2:183475/140‑1 (MQ=255)

                                                                                                                                  aaaGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGGGCCAg  <  2:383342/140‑1 (MQ=255)

                                                                                                                                  aaaGGTGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAg  <  1:168795/140‑1 (MQ=255)

GGACTGTCATAAACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAACTGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAG  >  NC_000913/4289394‑4289663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`