Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,307,253	A→G	C183R (TGC→CGC)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,307,253	0	A	G	100.0%	174.8 / NA	48	C183R (TGC→CGC)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/19);  total (29/19)

GTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCACCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAC  >  NC_000913/4307118‑4307381                                                                                                                                         |                                                                                                                                  gTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCa                                                                                                                               >  1:691402/1‑140 (MQ=255)  tGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAg                                                                                                                              >  2:18950/1‑140 (MQ=255)           ttCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCcac                                                                                                                     <  1:880750/140‑1 (MQ=255)            tCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCcaca                                                                                                                    >  2:791654/1‑140 (MQ=255)            tCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGCCGCCCAGGATTGCcaca                                                                                                                    >  2:439667/1‑140 (MQ=255)               aGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACAcgc                                                                                                                 <  2:248019/140‑1 (MQ=255)                gCAGACTCTGGACGAAAGGGGCGTTTGCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACAcgcg                                                                                                                <  2:1199/140‑1 (MQ=255)                 cAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACgcgc                                                                                                               <  1:195208/140‑1 (MQ=255)                 cAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACgcgc                                                                                                               <  1:616126/140‑1 (MQ=255)                   gACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAg                                                                                                             <  2:949518/140‑1 (MQ=255)                    aCTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAgt                                                                                                            <  2:519535/140‑1 (MQ=255)                     ctctGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAgtg                                                                                                           <  1:791654/140‑1 (MQ=255)                      tctGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACAcgcg                                                                                                                >  1:84686/1‑134 (MQ=255)                      tctGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACAcgcg                                                                                                                <  2:84686/134‑1 (MQ=255)                      tctGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAgtgt                                                                                                          <  1:543709/140‑1 (MQ=255)                        tGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAgtg                                                                                                           >  1:969308/1‑137 (MQ=255)                        tGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAgtg                                                                                                           <  2:969308/137‑1 (MQ=255)                           aCGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGt                                                                                                    >  2:705627/1‑140 (MQ=255)                              aaaGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCa                                                                                                              >  2:503563/1‑128 (MQ=255)                              aaaGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCa                                                                                                              <  1:503563/128‑1 (MQ=255)                               aaGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCata                                                                                                <  1:436839/140‑1 (MQ=255)                                aGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCatat                                                                                               <  1:1000289/140‑1 (MQ=255)                                aGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCGTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCatat                                                                                               >  2:807976/1‑140 (MQ=255)                                    gCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTcc                                                                                           <  1:18950/140‑1 (MQ=255)                                     cGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCatat                                                                                               <  1:1067521/135‑1 (MQ=255)                                     cGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCatat                                                                                               >  2:1067521/1‑135 (MQ=255)                                       ttttCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAg                                                                                        <  1:705627/140‑1 (MQ=255)                                              cATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGata                                                                                 <  1:807976/140‑1 (MQ=255)                                                  gACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGata                                                                                 <  2:981100/136‑1 (MQ=255)                                                  gACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGata                                                                                 >  1:981100/1‑136 (MQ=255)                                                        aaGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGtt                                                                       >  1:632879/1‑140 (MQ=255)                                                             cgcgCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCt                                                                  >  1:569783/1‑140 (MQ=255)                                                               cgcAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGc                                                                >  1:705999/1‑140 (MQ=255)                                                                          aTTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGtgtg                                                     >  1:193924/1‑140 (MQ=255)                                                                              cGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAcc                                                 >  1:600267/1‑140 (MQ=255)                                                                              cGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAcc                                                 >  1:1074659/1‑140 (MQ=255)                                                                                                cAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGtt                               >  1:127829/1‑140 (MQ=255)                                                                                                  gCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCa                             >  2:910901/1‑140 (MQ=255)                                                                                                   cGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCAt                            >  2:818972/1‑140 (MQ=255)                                                                                                     tCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATcc                          >  1:840767/1‑140 (MQ=255)                                                                                                      tttAGCGCCATTCAAGAGCAATTGGTTTCC‑GGGCGCCCAGGATTCCCACACGCGCGGTGTG‑GGTTAATATCCCCGGGGGGGATATGACCCAGTTCCCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCca                         <  2:193924/139‑1 (MQ=255)                                                                                                        tAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCcaca                       >  1:95119/1‑140 (MQ=255)                                                                                                        tAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCcaca                       >  1:859378/1‑140 (MQ=255)                                                                                                        tAGCCCCATCCCAGAGCAATTGGTTCCC‑AGGCGCCCAGGATTCCCACCCGCGCAGTGTT‑GGTTCATCCCCCCCGGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCcaca                       <  2:95119/140‑1 (MQ=255)                                                                                                         aGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCacac                      >  2:325413/1‑140 (MQ=255)                                                                                                                ttCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCg                                 >  2:89124/1‑122 (MQ=255)                                                                                                                ttCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCg                                 <  1:89124/122‑1 (MQ=255)                                                                                                                ttCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAc               >  2:1128445/1‑140 (MQ=255)                                                                                                                ttCCAGAGCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAc               >  1:356130/1‑140 (MQ=255)                                                                                                                  ccAGAGCATTTGGTTCCC‑AGGCGCCCAGCATTCCCCCCCGCGCAGTGTT‑GGCTAATATCCCCCAGGGGGATCGGACCCCGTCCCCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAccc             <  2:127829/140‑1 (MQ=255)                                                                                                                  ccAGAGCAATTGGTTTCC‑GGGCGCCCAGGATTCCCACGCGCGCAGTGTT‑GGGTCATATCTCCCGGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAccc             <  2:1074659/140‑1 (MQ=255)                                                                                                                   cAGAGCAATTGGTTCCC‑GGGCGCCCAGGATGGCCACACGCGCAGTGTT‑GGGTCATACCCCCCGGGGGGATATGCCCCAGTCCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAcccc            <  2:600267/140‑1 (MQ=255)                                                                                                                    agagCAATTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCa           >  2:340631/1‑140 (MQ=255)                                                                                                                       gcttttGTTTCCC‑GGGCGCCCAGGATGGCCCCCCGCGCGGTGTG‑GGGTAATTCCCCCCGGGGGGATATGCCCCAGTTCCCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATcc        <  2:632879/136‑1 (MQ=255)                                                                                                                         atttGGTTCCC‑GGGCCCCCGGGTTTCCCACCCGCG‑GGTGTTGGGGTAATACCTCCGGGGGGGATATGACCCAGTCCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATcccc      <  2:823700/138‑1 (MQ=255)                                                                                                                          aTTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCg     >  1:823700/1‑140 (MQ=255)                                                                                                                          aTTGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCg     >  1:1045431/1‑140 (MQ=255)                                                                                                                           ttGGTTTCC‑AGGCGCCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt    >  2:687013/1‑140 (MQ=255)                                                                                                                            tGGTTTCC‑AGGCCCCAAGGTTCGCCCCCCGGGCGGTGTT‑GGTTCATCTCCCCCGGGGGGATATGACCCTTTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa   <  2:296742/140‑1 (MQ=255)                                                                                                                              gTTTCCGAGGCGCCAAGGATGGCCACGCGCGGGGTGTT‑GGGTCATATCCCCCGGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc  <  2:840767/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCC‑AGGCACCCAGGATTGCCACACGCGCAGTGTT‑GGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAC  >  NC_000913/4307118‑4307381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑