Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,311,369 T→C D225G (GAC→GGC)  alsB ← D‑allose ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,311,3690TC100.0% 191.4 / NA 52D225G (GAC→GGC) alsBD‑allose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/29);  total (23/29)

CTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTT  >  NC_000913/4311233‑4311504
                                                                                                                                        |                                                                                                                                       
cTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCAt                                                                                                                                      <  1:239006/140‑1 (MQ=255)
  gggCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTc                                                                                                                                    <  2:198408/140‑1 (MQ=255)
   ggCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTcg                                                                                                                                   <  1:624428/140‑1 (MQ=255)
        ccGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCaa                                                                                                                              <  1:921389/140‑1 (MQ=255)
         cGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTcg                                                                                                                                   <  1:696726/134‑1 (MQ=255)
         cGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTcg                                                                                                                                   >  2:696726/1‑134 (MQ=255)
             gcgGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGAt                                                                                                                         <  2:1049276/140‑1 (MQ=255)
             gcgGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGAt                                                                                                                         <  2:730345/140‑1 (MQ=255)
             gcgGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGAt                                                                                                                         <  2:70591/140‑1 (MQ=255)
                      tGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAat                                                                                                                >  2:738694/1‑140 (MQ=255)
                        tCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAatat                                                                                                              >  1:967484/1‑140 (MQ=255)
                             cTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGAt                                                                                                                         <  2:627676/124‑1 (MQ=255)
                             cTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGAt                                                                                                                         >  1:627676/1‑124 (MQ=255)
                             cTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGa                                                                                                         <  1:344278/140‑1 (MQ=255)
                             cTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGa                                                                                                         <  2:639748/140‑1 (MQ=255)
                               tCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGAtt                                                                                                       >  2:988169/1‑140 (MQ=255)
                                 caccaTTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTAc                                                                                                     <  2:470473/140‑1 (MQ=255)
                                      ttttGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGc                                                                                                <  1:837521/140‑1 (MQ=255)
                                       tttGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCa                                                                                               >  1:1077633/1‑140 (MQ=255)
                                        ttGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCaa                                                                                              <  1:145072/140‑1 (MQ=255)
                                           cGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAacac                                                                                           >  2:1162145/1‑140 (MQ=255)
                                             ggCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAaca                                                                                            >  2:847555/1‑137 (MQ=255)
                                             ggCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAaca                                                                                            <  1:847555/137‑1 (MQ=255)
                                                  ccGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTg                                                                                    <  2:347738/140‑1 (MQ=255)
                                                  ccGGAATGCAATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTg                                                                                    <  2:90604/140‑1 (MQ=255)
                                                       aTGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGAc                                                                               <  2:967484/140‑1 (MQ=255)
                                                        tGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACa                                                                              <  1:1076118/140‑1 (MQ=255)
                                                            aTCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCa                                                                          <  1:738694/140‑1 (MQ=255)
                                                               tGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTg                                                                       >  2:498965/1‑140 (MQ=255)
                                                                gTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCa                                                                          <  2:1135790/136‑1 (MQ=255)
                                                                gTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCa                                                                          >  1:1135790/1‑136 (MQ=255)
                                                                  aCCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATc                                                                            >  1:55124/1‑132 (MQ=255)
                                                                  aCCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATc                                                                            <  2:55124/132‑1 (MQ=255)
                                                                  aCCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCtt                                                                    >  1:74012/1‑140 (MQ=255)
                                                                    cgacgaCCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACa                                                                              <  1:1004575/128‑1 (MQ=255)
                                                                    cgacgaCCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACa                                                                              >  2:1004575/1‑128 (MQ=255)
                                                                       cgacCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATg                                                               <  2:74012/140‑1 (MQ=255)
                                                                       cgacCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATg                                                               <  1:417030/140‑1 (MQ=255)
                                                                        gacCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGc                                                              >  2:909329/1‑140 (MQ=255)
                                                                           cAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGt                                                           >  2:1121313/1‑140 (MQ=255)
                                                                                   ttCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCg                                                   >  1:930021/1‑140 (MQ=255)
                                                                                             tCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAggctggc                                         >  1:404219/1‑140 (MQ=255)
                                                                                                  cGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGGGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCg                                    <  2:561723/140‑1 (MQ=255)
                                                                                                            tGCCTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGa                          >  2:53136/1‑140 (MQ=255)
                                                                                                              ccTGAGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATc                        >  1:604325/1‑140 (MQ=255)
                                                                                                                  aGCAACACCCATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGgc                    <  2:404219/140‑1 (MQ=255)
                                                                                                                         cccATTGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCttt             >  1:980208/1‑140 (MQ=255)
                                                                                                                              tGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCtttttgaa        >  2:1122469/1‑137 (MQ=255)
                                                                                                                              tGCCATCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGa        <  1:988169/140‑1 (MQ=255)
                                                                                                                                   tCGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCt   <  2:980208/140‑1 (MQ=255)
                                                                                                                                    cGTGCCATTCGCGCATTAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCtt  >  1:323991/1‑140 (MQ=255)
                                                                                                                                    cGTGCCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCtt  >  2:25215/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTT  >  NC_000913/4311233‑4311504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑