BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,379,256	A→G	G37G (GGT→GGC)	frdD ←	fumarate reductase membrane protein FrdD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,379,256	0	A	G	100.0%	405.7 / NA	110	G37G (GGT→GGC)	frdD	fumarate reductase membrane protein FrdD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (45/65); total (45/65)

Only 100 of 113 total aligned reads displayed.

TACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCA  >  NC_000913/4379120‑4379392

tACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGccc                                                                                                                                       <  2:71107/140‑1 (MQ=255)

tACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGccc                                                                                                                                       <  2:791043/140‑1 (MQ=255)

 aCGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCcca                                                                                                                                      <  1:1117436/140‑1 (MQ=255)

 aCGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCcca                                                                                                                                      >  2:402435/1‑140 (MQ=255)

   ggTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCccacc                                                                                                                                    >  2:648860/1‑140 (MQ=255)

    gtgtAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCccacca                                                                                                                                   <  2:1114828/140‑1 (MQ=255)

    gtgtAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCccacca                                                                                                                                   <  2:150837/140‑1 (MQ=255)

    gtgtAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCccacca                                                                                                                                   <  2:954898/140‑1 (MQ=255)

      gtAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGccc                                                                                                                                       >  2:137346/1‑134 (MQ=255)

      gtAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACcagc                                                                                                                                 <  1:391669/140‑1 (MQ=255)

       tAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACcagca                                                                                                                                <  1:648860/140‑1 (MQ=255)

       tAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACcagca                                                                                                                                >  1:764768/1‑140 (MQ=255)

              caccacaGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGatcat                                                                                                                         >  1:331161/1‑140 (MQ=255)

               accacaGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGatcatc                                                                                                                        >  1:676889/1‑140 (MQ=255)

               accacaGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGatcatc                                                                                                                        >  2:1656/1‑140 (MQ=255)

               accacaGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGatcatc                                                                                                                        >  2:157053/1‑140 (MQ=255)

                 cacaGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCAc                                                                                                                      <  1:340671/140‑1 (MQ=255)

                 cacaGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCAc                                                                                                                      <  2:97521/140‑1 (MQ=255)

                 cacaGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCAc                                                                                                                      <  1:653866/140‑1 (MQ=255)

                   caGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAAAGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCg                                                                                                                    >  1:1016862/1‑140 (MQ=255)

                    aGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCgg                                                                                                                   >  1:187885/1‑140 (MQ=255)

                    aGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCgg                                                                                                                   >  1:125021/1‑140 (MQ=255)

                      cGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGgcg                                                                                                                 >  1:175009/1‑140 (MQ=255)

                      cGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGgcg                                                                                                                 >  1:816760/1‑140 (MQ=255)

                         cAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCaa                                                                                                              >  2:1163418/1‑140 (MQ=255)

                         cAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCaa                                                                                                              >  1:181269/1‑140 (MQ=255)

                           gAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAatg                                                                                                            >  1:1149027/1‑140 (MQ=255)

                            aaCGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAatga                                                                                                           <  1:157053/140‑1 (MQ=255)

                              cGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAatgatg                                                                                                         <  2:187885/140‑1 (MQ=255)

                               gATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATgg                                                                                                        <  2:8402/140‑1 (MQ=255)

                                atcatcAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGgc                                                                                                       <  2:1016862/140‑1 (MQ=255)

                                 tcatcaGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCGCCGGCGCAATGATGgcg                                                                                                      <  1:1089702/140‑1 (MQ=255)

                                 tcatcaGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGgcg                                                                                                      <  2:676889/140‑1 (MQ=255)

                                   atcaGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCt                                                                                                    <  2:1126834/140‑1 (MQ=255)

                                   atcaGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCt                                                                                                    <  1:274880/140‑1 (MQ=255)

                                     caggaacaggaaTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTcc                                                                                                  <  2:279217/140‑1 (MQ=255)

                                      aggaacaggaaTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCt                                                                                                    >  2:277200/1‑137 (MQ=255)

                                      aggaacaggaaTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCt                                                                                                    <  1:277200/137‑1 (MQ=255)

                                       ggaacaggaaTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCcac                                                                                                >  2:653430/1‑140 (MQ=255)

                                           caggaaTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATac                                                                                            >  2:952131/1‑140 (MQ=255)

                                            aggaaTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATacc                                                                                           >  2:1128762/1‑140 (MQ=255)

                                             ggaaTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGgcg                                                                                                      <  1:711651/128‑1 (MQ=255)

                                             ggaaTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGgcg                                                                                                      >  2:711651/1‑128 (MQ=255)

                                                aTACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCg                                                                                       <  2:125021/140‑1 (MQ=255)

                                                  aCGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGgcg                                                                                                      <  1:754704/123‑1 (MQ=255)

                                                  aCGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGgcg                                                                                                      >  2:754704/1‑123 (MQ=255)

                                                   cgcgACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGcc                                                                                    >  1:1046297/1‑140 (MQ=255)

                                                     cgACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGcccc                                                                                  <  1:454143/140‑1 (MQ=255)

                                                      gACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCg                                                                                 >  1:955069/1‑140 (MQ=255)

                                                      gACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCg                                                                                 >  1:224468/1‑140 (MQ=255)

                                                      gACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCg                                                                                 >  1:212740/1‑140 (MQ=255)

                                                        ccAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGaa                                                                               <  1:426176/140‑1 (MQ=255)

                                                        ccAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGaa                                                                               <  2:816760/140‑1 (MQ=255)

                                                         cAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAag                                                                              <  2:764768/140‑1 (MQ=255)

                                                            tGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAgg                                                                           <  2:175009/140‑1 (MQ=255)

                                                                  tctGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGccc                                                                        >  2:975773/1‑137 (MQ=255)

                                                                     gcgcGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAAt                                                                  >  1:960665/1‑140 (MQ=255)

                                                                     gcgcGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAAt                                                                  >  1:683208/1‑140 (MQ=255)

                                                                      cgcgAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATa                                                                 >  2:561557/1‑140 (MQ=255)

                                                                      cgcgAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATa                                                                 >  1:328891/1‑140 (MQ=255)

                                                                      cgcgAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATa                                                                 >  2:926328/1‑140 (MQ=255)

                                                                       gcgAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATAc                                                                <  2:683208/140‑1 (MQ=255)

                                                                       gcgAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATAc                                                                <  2:960665/140‑1 (MQ=255)

                                                                          aaCGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCgg                                                             <  2:517112/140‑1 (MQ=255)

                                                                            cGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGtt                                                           <  2:766441/140‑1 (MQ=255)

                                                                                   aCGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGa                                                    <  1:1656/140‑1 (MQ=255)

                                                                                       gcTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGc                                                >  1:304918/1‑140 (MQ=255)

                                                                                       gcTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGc                                                >  1:660740/1‑140 (MQ=255)

                                                                                       gcTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGc                                                >  2:448154/1‑140 (MQ=255)

                                                                                         tCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtt                                              <  2:1046297/140‑1 (MQ=255)

                                                                                         tCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtt                                              <  1:1163418/140‑1 (MQ=255)

                                                                                           gTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCACCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttg                                            >  2:538364/1‑140 (MQ=255)

                                                                                                 cAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttggatttg                                      >  2:407180/1‑140 (MQ=255)

                                                                                                   gcgcATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttgga                                          >  1:509983/1‑134 (MQ=255)

                                                                                                   gcgcATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttgga                                          <  2:509983/134‑1 (MQ=255)

                                                                                                     gcATCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTtggatttggatt                                  >  1:714502/1‑140 (MQ=255)

                                                                                                       aTCACCCGGAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTaa                                <  2:328891/140‑1 (MQ=255)

                                                                                                               gAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCa                        <  2:955069/140‑1 (MQ=255)

                                                                                                               gAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCa                        <  2:660740/140‑1 (MQ=255)

                                                                                                               gAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCa                        <  1:926328/140‑1 (MQ=255)

                                                                                                               gAAACAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCa                        <  1:441992/140‑1 (MQ=255)

                                                                                                                aaaCAACCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGACGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCtttggatttgga                                    >  2:733803/1‑127 (MQ=255)

                                                                                                                 aacaacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAgg                      <  2:331161/140‑1 (MQ=255)

                                                                                                                 aacaacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAgg                      <  1:402435/140‑1 (MQ=255)

                                                                                                                    aacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGccc                   <  2:224468/140‑3 (MQ=255)

                                                                                                                    aacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                   <  1:243330/140‑1 (MQ=255)

                                                                                                                    aacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                   <  1:1128762/140‑1 (MQ=255)

                                                                                                                    aacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                   <  2:104917/140‑1 (MQ=255)

                                                                                                                    aacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                   <  1:448154/140‑1 (MQ=255)

                                                                                                                    aacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                   <  1:538364/140‑1 (MQ=255)

                                                                                                                    aacCCCAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                   <  2:304918/140‑1 (MQ=255)

                                                                                                                         cAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTAc              <  1:653430/140‑1 (MQ=255)

                                                                                                                         cAGTGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTAc              <  2:649366/140‑1 (MQ=255)

                                                                                                                            tGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCa                        <  1:9862/127‑1 (MQ=255)

                                                                                                                            tGGCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAg           >  2:502178/1‑140 (MQ=255)

                                                                                                                             ggCAGCAGAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGt          >  2:829476/1‑140 (MQ=255)

                                                                                                                                agcagAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                   >  1:545053/1‑128 (MQ=255)

                                                                                                                                agcagAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTc                   <  2:545053/128‑1 (MQ=255)

                                                                                                                                 gcagAATGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAg      >  1:960806/1‑140 (MQ=255)

                                                                                                                                     aaTGCCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGca  <  1:829476/140‑1 (MQ=255)

TACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAACCCCAGTGGCAGCAGAATACCCACCAGCAGGATCATCACCGGCGCAATGATGGCGCTCCACATACCACCGGCCCCGAAGAGGCCCCAGAATACCGGTTCGTCAGAACGCTTTGGATTTGGATTAATCATCTCAGGCTCCTTACCAGTACAGGGCA  >  NC_000913/4379120‑4379392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`