Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,466,074	A→G	V36A (GTC→GCC)	treB ←	trehalose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,466,074	0	A	G	100.0%	317.8 / NA	87	V36A (GTC→GCC)	treB	trehalose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/45);  total (42/45)

GCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGG  >  NC_000913/4465939‑4466208                                                                                                                                        |                                                                                                                                       gCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATGGGCCGGTTGGTTGAGGGCaaa                                                                                                                                    >  2:1058161/1‑140 (MQ=255)    aTCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCg                                                                                                                                 <  1:1058161/140‑1 (MQ=255)    aTCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCg                                                                                                                                 >  1:222465/1‑140 (MQ=255)     tCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGt                                                                                                                                <  2:357875/140‑1 (MQ=255)       aGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAg                                                                                                                              <  2:232769/140‑1 (MQ=255)        gTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAgg                                                                                                                             <  2:727489/140‑1 (MQ=255)                   tAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCg                                                                                                                                 <  2:1065273/125‑1 (MQ=255)                   tAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCg                                                                                                                                 >  1:1065273/1‑125 (MQ=255)                   tAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCg                                                                                                                           <  2:735962/131‑1 (MQ=255)                   tAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCg                                                                                                                           >  1:735962/1‑131 (MQ=255)                   tAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACa                                                                                                                  <  2:446741/140‑1 (MQ=255)                     aTCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGt                                                                                                                >  2:944646/1‑140 (MQ=255)                         cccACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCt                                                                                                            <  2:554828/140‑1 (MQ=255)                          ccACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTc                                                                                                           >  1:1008496/1‑140 (MQ=255)                          ccACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTc                                                                                                           >  1:557559/1‑140 (MQ=255)                            aCGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCAc                                                                                                         <  1:944646/140‑1 (MQ=255)                             cGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTaa                                                                                                                      >  1:885053/1‑126 (MQ=255)                             cGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTaa                                                                                                                      <  2:885053/126‑1 (MQ=255)                              gTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCg                                                                                                       >  2:279903/1‑140 (MQ=255)                               ttGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGt                                                                                                      <  2:294360/140‑1 (MQ=255)                               ttGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGt                                                                                                      <  1:624335/140‑1 (MQ=255)                                 ggTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCg                                                                                                    <  2:237265/140‑1 (MQ=255)                                 ggTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCg                                                                                                    <  1:678712/140‑1 (MQ=255)                                             caccTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCcgc                                                                                        <  2:864510/140‑1 (MQ=255)                                                  gAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGcgcccg                                                                                   <  2:557559/140‑1 (MQ=255)                                                  gAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGcgcccg                                                                                   <  1:290430/140‑1 (MQ=255)                                                  gAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGcgcccg                                                                                   >  2:396428/1‑140 (MQ=255)                                                    aaTTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGcgcccgcc                                                                                 >  2:235568/1‑140 (MQ=255)                                                      ttGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCCCCGTCGCAATATTGCCGCGCCCGCCGa                                                                               <  1:1071537/140‑1 (MQ=255)                                                      ttGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGa                                                                               <  2:222465/140‑1 (MQ=255)                                                      ttGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGa                                                                               <  2:508791/140‑1 (MQ=255)                                                      ttGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGa                                                                               <  2:86233/140‑1 (MQ=255)                                                       tGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAc                                                                              <  1:396428/140‑1 (MQ=255)                                                         cccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGa                                                                               >  1:832953/1‑137 (MQ=255)                                                         cccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGa                                                                               <  2:832953/137‑1 (MQ=255)                                                         cccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCa                                                                            >  2:136592/1‑140 (MQ=255)                                                         cccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCa                                                                            >  1:880169/1‑140 (MQ=255)                                                         cccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCa                                                                            >  1:223476/1‑140 (MQ=255)                                                         cccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCa                                                                            >  1:526338/1‑140 (MQ=255)                                                          ccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAg                                                                           <  1:235568/140‑1 (MQ=255)                                                          ccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAg                                                                           >  1:468624/1‑140 (MQ=255)                                                              cATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCcgc                                                                                        <  2:322452/123‑1 (MQ=255)                                                              cATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCcgc                                                                                        >  1:322452/1‑123 (MQ=255)                                                              cATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTtca                                                                       <  2:1125314/140‑1 (MQ=255)                                                                ttGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTtcaat                                                                     <  1:136592/140‑1 (MQ=255)                                                                 tGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTtcaatc                                                                    <  1:534685/140‑1 (MQ=255)                                                                     gAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAAcc                                                                <  2:880169/140‑1 (MQ=255)                                                                     gAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAAcc                                                                <  2:223476/140‑1 (MQ=255)                                                                        aCAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACcgat                                                             <  2:468624/140‑1 (MQ=255)                                                                             cTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGata                                                        >  1:588753/1‑140 (MQ=255)                                                                             cTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGata                                                        >  2:17309/1‑140 (MQ=255)                                                                             cTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGata                                                        >  1:333529/1‑140 (MQ=255)                                                                                      tAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTgg                                               >  1:790784/1‑140 (MQ=255)                                                                                         ggAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGttt                                            >  1:39931/1‑140 (MQ=255)                                                                                         ggAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGttt                                            >  2:73206/1‑140 (MQ=255)                                                                                          gAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTa                                           >  2:1098362/1‑140 (MQ=255)                                                                                           aGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGt                                              >  2:258593/1‑136 (MQ=255)                                                                                           aGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGt                                              <  1:258593/136‑1 (MQ=255)                                                                                            gTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAtt                                         <  1:73206/140‑1 (MQ=255)                                                                                             ttGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAttt                                        >  1:865602/1‑140 (MQ=255)                                                                                              tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTa                                           <  2:88931/136‑1 (MQ=255)                                                                                              tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTa                                           >  1:88931/1‑136 (MQ=255)                                                                                              tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAtttt                                       >  2:749082/1‑140 (MQ=255)                                                                                               gCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTg                                      <  2:1008496/140‑1 (MQ=255)                                                                                               gCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTg                                      >  1:825991/1‑140 (MQ=255)                                                                                                cTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGc                                     >  2:347691/1‑140 (MQ=255)                                                                                                 tCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCt                                    <  2:218282/140‑1 (MQ=255)                                                                                                 tCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCt                                    <  2:526338/140‑1 (MQ=255)                                                                                                 tCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCt                                    <  1:965505/140‑1 (MQ=255)                                                                                                 tCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCt                                    <  2:790784/140‑1 (MQ=255)                                                                                                   aaTTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCtca                                  <  2:865602/140‑1 (MQ=255)                                                                                                   aaTTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCtca                                  >  1:610461/1‑140 (MQ=255)                                                                                                   aaTTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCtca                                  <  1:279903/140‑1 (MQ=255)                                                                                                     tttctttcGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCt                                    >  2:26979/1‑136 (MQ=255)                                                                                                     tttctttcGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCt                                    <  1:26979/136‑1 (MQ=255)                                                                                                       tctttcGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGt                                              >  2:955051/1‑124 (MQ=255)                                                                                                       tctttcGGTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGt                                              <  1:955051/124‑1 (MQ=255)                                                                                                             ggTCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTACTCATCATAAAGcc                        >  2:749334/1‑140 (MQ=255)                                                                                                               tCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATaa                            >  2:198189/1‑134 (MQ=255)                                                                                                               tCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATaa                            <  1:198189/134‑1 (MQ=255)                                                                                                               tCTGGCATTGGCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGcccc                      >  2:1001354/1‑140 (MQ=255)                                                                                                                        ggCCGGTTGGTTGAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGAt             >  2:1140718/1‑140 (MQ=255)                                                                                                                              ttggttgAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGa              >  2:709646/1‑133 (MQ=255)                                                                                                                              ttggttgAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGa              >  2:794259/1‑133 (MQ=255)                                                                                                                              ttggttgAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGa              <  1:794259/133‑1 (MQ=255)                                                                                                                              ttggttgAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGa              <  1:709646/133‑1 (MQ=255)                                                                                                                              ttggttgAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACAtt       >  1:24849/1‑140 (MQ=255)                                                                                                                                   tgAGGGCAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGGTGACATTTttgg  >  1:1091948/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGG  >  NC_000913/4465939‑4466208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑