Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	357,589	G→A	intergenic (+135/+202)	codA → / ← cynR	cytosine/isoguanine deaminase/DNA‑binding transcriptional dual regulator CynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	357,589	0	G	A	100.0%	179.6 / NA	52	intergenic (+135/+202)	codA/cynR	cytosine/isoguanine deaminase/DNA‑binding transcriptional dual regulator CynR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/28);  total (24/28)

GGTTACAGCGAGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAG  >  NC_000913/357462‑357721                                                                                                                                |                                                                                                                                     ggTTACAGCGAGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGatt                                                                                                                          >  2:749416/1‑140 (MQ=255)      cAGCGAGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGattcattc                                                                                                                     <  2:618108/140‑1 (MQ=255)       aGCGAGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTcc                                                                                                                    <  2:826910/140‑1 (MQ=255)       aGCGAGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTcc                                                                                                                    <  2:35879/140‑1 (MQ=255)        gCGAGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTccc                                                                                                                   <  1:99294/140‑1 (MQ=255)           aGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGcc                                                                                                                <  1:749416/140‑1 (MQ=255)           aGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGcc                                                                                                                >  1:71807/1‑140 (MQ=255)           aGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGcc                                                                                                                >  2:530069/1‑140 (MQ=255)             cTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCcaa                                                                                                              >  1:403848/1‑140 (MQ=255)                  tttATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAAcc                                                                                                         <  2:1150707/140‑1 (MQ=37)                      tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTccc                                                                                                                   <  1:505395/126‑1 (MQ=255)                      tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTccc                                                                                                                   >  2:505395/1‑126 (MQ=38)                        ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCAt                                                                                                   <  2:222373/140‑1 (MQ=38)                        ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCAt                                                                                                   <  2:1046621/140‑1 (MQ=38)                           gATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATtcc                                                                                                >  1:457234/1‑140 (MQ=255)                            aTGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATtcct                                                                                               >  1:880607/1‑140 (MQ=255)                                    tGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGcc                                                                                       <  2:403848/140‑1 (MQ=255)                                    tGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGcc                                                                                       <  1:530069/140‑1 (MQ=255)                                            ttATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCaa                                                                               >  2:225529/1‑140 (MQ=255)                                               tCCGGCTTACTTAGCGCCCTGAACTCGTAGCGCTGTTAAGCGTAGCACATCAGCCATTTCCAGCCAATGATCTGTGTCAGCAGCTTCCGTCATTCATTCCCGCCAAAAACCGCGCATTCCTCCAACGCCATGTGCaaaaa                                                                            <  2:735730/140‑1 (MQ=14)                                                       aCGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCtt                                                                      <  2:893309/138‑1 (MQ=255)                                                       aCGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCtt                                                                      >  1:893309/1‑138 (MQ=255)                                                              gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGc                                                             >  1:64585/1‑140 (MQ=255)                                                                  tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                         >  2:1111442/1‑140 (MQ=255)                                                                  tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                         >  2:1131700/1‑140 (MQ=255)                                                                  tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                         >  1:273122/1‑140 (MQ=255)                                                                   gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCt                                                        >  1:778326/1‑140 (MQ=255)                                                                    aaCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCt                                                        >  2:1002770/1‑139 (MQ=255)                                                                    aaCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCt                                                        <  1:1002770/139‑1 (MQ=255)                                                                    aaCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTg                                                       >  2:1020725/1‑140 (MQ=255)                                                                      cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGcc                                                     <  1:1111442/140‑1 (MQ=255)                                                                          tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                 <  1:1131700/140‑1 (MQ=255)                                                                           aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTg                                                <  2:880607/140‑1 (MQ=255)                                                                           aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTg                                                <  1:570172/140‑1 (MQ=255)                                                                              ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAg                                             >  1:597888/1‑140 (MQ=255)                                                                                     aGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGc                                      <  2:64585/140‑1 (MQ=255)                                                                                            cgcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAATAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgc                               >  2:413928/1‑140 (MQ=255)                                                                                            cgcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGTCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgc                               >  1:988044/1‑140 (MQ=255)                                                                                              cATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGa                                  >  2:403215/1‑135 (MQ=255)                                                                                              cATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGa                                  <  1:403215/135‑1 (MQ=255)                                                                                                 cAGGCAATTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGCCGt                          >  1:372593/1‑140 (MQ=255)                                                                                                      aaTTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGTCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACg                     >  1:433611/1‑140 (MQ=255)                                                                                                       aTTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                    <  2:372593/140‑1 (MQ=255)                                                                                                       aTTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                    <  2:764635/140‑1 (MQ=255)                                                                                                       aTTCCAGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                    <  2:597888/140‑1 (MQ=255)                                                                                                            aGCCGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGTCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt               <  2:988044/140‑1 (MQ=255)                                                                                                              ccGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATcc             >  1:477764/1‑140 (MQ=255)                                                                                                              ccGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATcc             <  2:273122/140‑1 (MQ=255)                                                                                                               cGCTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCg            <  2:71807/140‑1 (MQ=255)                                                                                                                 cTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAATCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGCTGCGGCGTGAACGCCTTATCCGGc          <  2:688969/140‑1 (MQ=255)                                                                                                                 cTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc          <  2:477764/140‑1 (MQ=255)                                                                                                                 cTGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc          <  2:457234/140‑1 (MQ=255)                                                                                                                  tGATCTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGcc         >  1:651336/1‑140 (MQ=255)                                                                                                                      cTGTGTCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGTCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg     <  2:433611/140‑1 (MQ=255)                                                                                                                         tgtCAGCAGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTag  <  1:417554/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                     GGTTACAGCGAGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAG  >  NC_000913/357462‑357721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑