Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,548,034	C→T	A98V (GCC→GTC)	fimF →	type 1 fimbriae minor subunit FimF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,548,034	0	C	T	100.0%	281.4 / NA	78	A98V (GCC→GTC)	fimF	type 1 fimbriae minor subunit FimF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/44);  total (34/44)

GAAAACGCGGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGCCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCTGACGCCGGGT  >  NC_000913/4547898‑4548167                                                                                                                                         |                                                                                                                                      gAAAACGCGGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCaa                                                                                                                                    <  1:189348/140‑1 (MQ=255) gAAAACGCGGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCaa                                                                                                                                    <  1:935778/140‑1 (MQ=255) gAAAACGCGGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCaa                                                                                                                                    <  1:657606/140‑1 (MQ=255)   aaaCGCGGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAAcc                                                                                                                                  >  2:212079/1‑140 (MQ=255)      cgcgGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGc                                                                                                                               >  2:26331/1‑140 (MQ=255)      cgcgGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGc                                                                                                                               >  2:805501/1‑140 (MQ=255)        cggcgAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCtt                                                                                                                             >  2:204374/1‑140 (MQ=255)         ggcgAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTg                                                                                                                            <  1:1087048/140‑1 (MQ=255)           cgAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCa                                                                                                                          >  2:221737/1‑140 (MQ=255)           cgAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCa                                                                                                                          <  2:105454/140‑1 (MQ=255)           cgAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCa                                                                                                                          <  1:212079/140‑1 (MQ=255)           cgAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCa                                                                                                                          <  1:26331/140‑1 (MQ=255)           cgAAGCAATTCAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCa                                                                                                                          <  1:796811/140‑1 (MQ=255)             aaGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACt                                                                                                                        <  1:250952/140‑1 (MQ=255)                   tttAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGaaaa                                                                                                                  >  2:660654/1‑140 (MQ=255)                   tttAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGaaaa                                                                                                                  <  2:1136736/140‑1 (MQ=255)                      aacaacATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATAc                                                                                                               >  2:612257/1‑140 (MQ=255)                             ttGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGCTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCa                                                                                                        >  2:836305/1‑140 (MQ=255)                                gcgcGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCg                                                                                                     >  2:151051/1‑140 (MQ=255)                                    gacgacTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCtt                                                                                                 <  2:723190/140‑1 (MQ=255)                                     acgacTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTc                                                                                                >  1:316639/1‑140 (MQ=255)                                     acgacTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTc                                                                                                >  1:410188/1‑140 (MQ=255)                                      cgacTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCg                                                                                               <  1:431246/140‑1 (MQ=255)                                      cgacTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCg                                                                                               >  1:831150/1‑140 (MQ=255)                                              gttgttCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTggg                                                                                       <  1:612257/140‑1 (MQ=255)                                                  ttCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATa                                                                                   <  2:410188/140‑1 (MQ=255)                                                  ttCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATa                                                                                   <  1:221737/140‑1 (MQ=255)                                                  ttCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATa                                                                                   <  1:894233/140‑1 (MQ=255)                                                       tttCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCggg                                                                                             >  1:685383/1‑125 (MQ=255)                                                       tttCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCggg                                                                                             <  2:685383/125‑1 (MQ=255)                                                       tttCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCt                                                                              >  1:914188/1‑140 (MQ=255)                                                       tttCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCAACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCt                                                                              >  2:465916/1‑140 (MQ=255)                                                            tATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGa                                                                         >  2:148340/1‑140 (MQ=255)                                                                 tGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGag                                                                    <  1:805501/140‑1 (MQ=255)                                                                   cTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATg                                                                      >  1:688045/1‑136 (MQ=255)                                                                   cTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATg                                                                      <  2:688045/136‑1 (MQ=255)                                                                    tGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGagcag                                                                 <  1:204374/140‑1 (MQ=255)                                                                    tGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGagcag                                                                 <  2:665101/140‑1 (MQ=255)                                                                      tCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAgcagca                                                               <  2:914188/140‑1 (MQ=255)                                                                         cccTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCaaaa                                                            <  2:530397/140‑1 (MQ=255)                                                                              tgGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCaaa                                                       <  1:93522/140‑1 (MQ=255)                                                                              tgGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCaaa                                                       <  2:264208/140‑1 (MQ=255)                                                                                   aTGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATAccc                                                  >  1:791803/1‑140 (MQ=255)                                                                                   aTGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATAccc                                                  >  2:442359/1‑140 (MQ=255)                                                                                    tGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATAcccc                                                 >  2:610589/1‑140 (MQ=255)                                                                                    tGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATAcccc                                                 >  1:1151745/1‑140 (MQ=255)                                                                                     gCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                <  1:926120/140‑1 (MQ=255)                                                                                     gCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                <  1:610589/140‑1 (MQ=255)                                                                                     gCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                <  1:527861/140‑1 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAgcagca                                                               <  1:895520/122‑1 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAgcagca                                                               >  2:895520/1‑122 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCGAAATCAAATACCCCTGaa                                             >  2:1005226/1‑140 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                <  2:752019/137‑1 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                <  1:985672/137‑1 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                >  2:985672/1‑137 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                >  1:752019/1‑137 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTaa                                             >  1:238847/1‑140 (MQ=255)                                                                                        gTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTaa                                             >  2:678941/1‑140 (MQ=255)                                                                                         tttCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAAt                                            >  2:335647/1‑140 (MQ=255)                                                                                           tCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                >  1:194263/1‑134 (MQ=255)                                                                                           tCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCt                                                <  2:194263/134‑1 (MQ=255)                                                                                           tCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGc                                          >  2:293882/1‑140 (MQ=255)                                                                                           tCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGc                                          <  2:791803/140‑1 (MQ=255)                                                                                             tGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTc                                        <  1:293882/140‑1 (MQ=255)                                                                                                cGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCAt                                     >  1:954166/1‑140 (MQ=255)                                                                                                                aCTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTg                     <  2:16141/140‑1 (MQ=255)                                                                                                                 cTGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTgg                    <  2:491204/140‑1 (MQ=255)                                                                                                                  tGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCcgcg                             >  2:1038109/1‑130 (MQ=255)                                                                                                                  tGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCcgcg                             <  1:1038109/130‑1 (MQ=255)                                                                                                                  tGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGga                   >  1:894129/1‑140 (MQ=255)                                                                                                                  tGGCGTTGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGga                   <  2:894129/140‑1 (MQ=255)                                                                                                                       ttGCAGATAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGAcc              >  1:1111595/1‑140 (MQ=255)                                                                                                                         gCAGATAGCAACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCt            <  1:465916/140‑1 (MQ=255)                                                                                                                             aTAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCTGACg        <  1:836305/140‑1 (MQ=255)                                                                                                                             aTAGCCACAATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCTGACg        <  2:238847/140‑1 (MQ=255)                                                                                                                                  cacaATGTCAACCTGCTTGCACTTGTAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCTGAc         <  2:1011941/134‑1 (MQ=255)                                                                                                                                  cacaATGTCAACCTGCTTGCACTTGTAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCTGAc         >  1:1011941/1‑134 (MQ=255)                                                                                                                                  cacaATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCTGACGCCggg   <  1:442359/140‑1 (MQ=255)                                                                                                                                   acaATGTCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCTGACGCCGGGt  <  2:1151745/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GAAAACGCGGCGAAGCAATTTAACAACATTGGCGCGACGACTCCTGTTGTTCCATTTCGTATTTTGCTGTCACCCTGTGGTAATGCCGTTTCTGCCGTAAAGGTTGGGTTTACTGGCGTTGCAGATAGCCACAATGCCAACCTGCTTGCACTTGAAAATACGGTGTCAGCGGCTTCGGGACTGGGAATACAGCTTCTGAATGAGCAGCAAAATCAAATACCCCTTAATGCTCCATCGTCCGCGCTTTCGTGGACGACCCTGACGCCGGGT  >  NC_000913/4547898‑4548167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑