Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,602,599	T→C	H87R (CAC→CGC)	yjjP ←	putative succinate exporter YjjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,602,599	0	T	C	100.0%	313.9 / NA	86	H87R (CAC→CGC)	yjjP	putative succinate exporter YjjP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/49);  total (37/49)

TACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCA  >  NC_000913/4602465‑4602731                                                                                                                                       |                                                                                                                                     tACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTgg                                                                                                                                 >  1:352438/1‑140 (MQ=255) tACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTgg                                                                                                                                 >  2:352788/1‑140 (MQ=255)     gAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACtt                                                                                                                             <  2:178210/140‑1 (MQ=255)       aaCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCa                                                                                                                           >  2:439984/1‑140 (MQ=255)        aCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAg                                                                                                                          <  1:689621/140‑1 (MQ=255)         cAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGt                                                                                                                         >  2:536936/1‑140 (MQ=255)         cAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGt                                                                                                                         <  2:667790/140‑1 (MQ=255)          aGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTc                                                                                                                        <  1:147586/140‑1 (MQ=255)            gcgcAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCt                                                                                                                                   >  2:189741/1‑127 (MQ=255)            gcgcAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCt                                                                                                                                   <  1:189741/127‑1 (MQ=255)            gcgcAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTcac                                                                                                                      <  2:521832/140‑1 (MQ=255)            gcgcAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTcac                                                                                                                      <  2:757366/140‑1 (MQ=255)             cgcAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTcacc                                                                                                                     <  1:172021/140‑1 (MQ=255)             cgcAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTcacc                                                                                                                     >  2:101914/1‑140 (MQ=255)             cgcAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTcacc                                                                                                                     <  2:174110/140‑1 (MQ=255)                aaGAAAGGCCAACCATTAAGGCTACCATCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACcaca                                                                                                                  <  2:330980/140‑1 (MQ=255)                aaGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTcaccac                                                                                                                   <  1:109568/139‑1 (MQ=255)                aaGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTcaccac                                                                                                                   >  2:109568/1‑139 (MQ=255)                  gAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATg                                                                                                                <  1:748324/140‑1 (MQ=255)                  gAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATg                                                                                                                <  1:511301/140‑1 (MQ=255)                      ggCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCata                                                                                                            >  1:870505/1‑140 (MQ=255)                            ccATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATg                                                                                                      >  1:848767/1‑140 (MQ=255)                                     cTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCg                                                                                             >  2:283972/1‑140 (MQ=255)                                       aCCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTg                                                                                           >  1:464115/1‑140 (MQ=255)                                       aCCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTg                                                                                           >  1:452345/1‑140 (MQ=255)                                       aCCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTg                                                                                           >  2:435147/1‑140 (MQ=255)                                       aCCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTg                                                                                           >  1:1120323/1‑140 (MQ=255)                                            ccATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATCAATGCCGCGATCGTGAtttt                                                                                      >  1:106436/1‑140 (MQ=255)                                             cATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGAttttt                                                                                     >  2:416577/1‑140 (MQ=255)                                             cATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAAACCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGAttttt                                                                                     >  1:518202/1‑140 (MQ=255)                                              aTCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTa                                                                                    <  1:101914/140‑1 (MQ=255)                                                   gggTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACgtgt                                                                               <  2:848767/140‑1 (MQ=255)                                                   gggTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACgtgt                                                                               <  1:283972/140‑1 (MQ=255)                                                     gTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTATGTGTCg                                                                             <  2:1120323/140‑1 (MQ=255)                                                      tAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGa                                                                            >  1:1039832/1‑140 (MQ=255)                                                         cGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTGCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGt                                                                         >  2:723178/1‑140 (MQ=255)                                                         cGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACgtgt                                                                               <  1:729631/134‑1 (MQ=255)                                                         cGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACgtgt                                                                               >  2:729631/1‑134 (MQ=255)                                                         cGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGt                                                                         >  1:570573/1‑140 (MQ=255)                                                             aTGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATCAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAgg                                                                     <  2:106436/140‑1 (MQ=255)                                                              tGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGc                                                                    >  2:556441/1‑140 (MQ=255)                                                              tGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGc                                                                    >  1:680919/1‑140 (MQ=255)                                                                   gAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGt                                                                         >  1:531296/1‑130 (MQ=255)                                                                   gAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGt                                                                         <  2:531296/130‑1 (MQ=255)                                                                   gAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCAttac                                                               <  1:335150/140‑3 (MQ=255)                                                                   gAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGc                                                               <  1:556441/140‑1 (MQ=255)                                                                   gAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGc                                                               <  2:196692/140‑1 (MQ=255)                                                                    aaTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCAT‑TTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  2:156519/140‑1 (MQ=255)                                                                     aTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  1:536896/140‑1 (MQ=255)                                                                     aTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  2:424406/140‑1 (MQ=255)                                                                     aTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  2:452345/140‑1 (MQ=255)                                                                     aTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  1:301597/140‑1 (MQ=255)                                                                     aTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  1:723178/140‑1 (MQ=255)                                                                     aTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  2:680919/140‑1 (MQ=255)                                                                     aTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  2:243937/140‑1 (MQ=255)                                                                     aTTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGccc                                                             <  1:352788/140‑1 (MQ=255)                                                                      tttGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCa                                                            <  2:883648/140‑1 (MQ=255)                                                                      tttGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCa                                                            <  2:870505/140‑1 (MQ=255)                                                                           cTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCttt                                                       >  1:772694/1‑140 (MQ=255)                                                                               aTCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATa                                                   <  1:416577/140‑1 (MQ=255)                                                                                           cGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACt                                       >  2:872672/1‑140 (MQ=255)                                                                                             ccTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTAt                                     <  2:710248/140‑1 (MQ=255)                                                                                              cTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGtt                                                <  1:1059595/128‑1 (MQ=255)                                                                                              cTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCGTTAATAGtt                                                >  2:1059595/1‑128 (MQ=255)                                                                                               tttGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTGTAATAGTTGTCAGCACTATgg                                   <  1:435147/140‑1 (MQ=255)                                                                                                ttGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATa                                                   <  2:704144/123‑1 (MQ=255)                                                                                                ttGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATa                                                   >  1:704144/1‑123 (MQ=255)                                                                                                ttGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGt                                              >  2:520623/1‑128 (MQ=255)                                                                                                ttGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGt                                              <  1:520623/128‑1 (MQ=255)                                                                                                 tGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCa                                            >  2:376968/1‑129 (MQ=255)                                                                                                 tGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCa                                            <  1:376968/129‑1 (MQ=255)                                                                                                 tGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCg                                 >  1:1005075/1‑140 (MQ=255)                                                                                                   tAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGtt                               <  2:870939/140‑1 (MQ=255)                                                                                                   tAATCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGtt                               <  2:1015814/140‑1 (MQ=255)                                                                                                      tCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCa                                            >  2:828779/1‑124 (MQ=255)                                                                                                      tCCAGCAGATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCa                                            <  1:828779/124‑1 (MQ=255)                                                                                                        cagcagATGATGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAag                          >  2:865342/1‑140 (MQ=255)                                                                                                             gatgatGCTCCGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATa                     <  1:748278/140‑1 (MQ=255)                                                                                                                       cGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGa           >  1:902903/1‑140 (MQ=255)                                                                                                                       cGCAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGa           >  2:972741/1‑140 (MQ=255)                                                                                                                         cAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACg         <  2:1005075/140‑1 (MQ=255)                                                                                                                         cAAGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACg         <  1:865342/140‑1 (MQ=255)                                                                                                                          aaGAATCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGc        <  1:972741/140‑1 (MQ=255)                                                                                                                              aTCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTc    >  2:352540/1‑140 (MQ=255)                                                                                                                               tCACAATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTcc   >  2:482504/1‑140 (MQ=255)                                                                                                                                cacaATGCGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCa  <  1:872672/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCTGGACTTCAGTCACCACATGCATATTAATGCCGCGATCGTGATTTTTACGTGTCGATGTCAGGCATTGCCCATCTTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCA  >  NC_000913/4602465‑4602731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑