Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	520,222	C→T	R164* (CGA→TGA)	ybbA →	putative ABC transporter ATP‑binding protein YbbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	520,222	0	C	T	100.0%	125.4 / NA	37	R164* (CGA→TGA)	ybbA	putative ABC transporter ATP‑binding protein YbbA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/21);  total (16/21)

TAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTG  >  NC_000913/520089‑520353                                                                                                                                      |                                                                                                                                    tAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTg                                                                                                                               <  1:43950/140‑1 (MQ=255) tAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTg                                                                                                                               <  1:615406/140‑1 (MQ=255)         aaaGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTg                                                                                                                               <  2:264636/132‑1 (MQ=255)         aaaGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTg                                                                                                                               >  1:264636/1‑132 (MQ=255)                      ggCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAAc                                                                                                          <  1:641868/140‑1 (MQ=255)                           aaGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCAcc                                                                                                     <  1:49098/140‑1 (MQ=255)                                          aGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGc                                                                                      >  1:741807/1‑140 (MQ=255)                                                    gggTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCg                                                                            >  2:253204/1‑140 (MQ=255)                                                     ggTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGa                                                                           >  1:17504/1‑140 (MQ=255)                                                      gTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGAt                                                                          >  2:696520/1‑140 (MQ=255)                                                      gTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGAt                                                                          >  1:169431/1‑140 (MQ=255)                                                       tAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGA‑CCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATaa                                                                        <  1:1109577/140‑1 (MQ=255)                                                               tGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGcc                                                                 >  2:961621/1‑140 (MQ=255)                                                               tGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGcc                                                                 >  1:187688/1‑140 (MQ=255)                                                                           cGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGttt                                                     <  2:472799/140‑1 (MQ=255)                                                                            ggCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGtttt                                                    >  2:154533/1‑140 (MQ=255)                                                                                caGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGCCCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCt                                                <  1:556085/140‑1 (MQ=255)                                                                                caGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCt                                                <  2:846355/140‑1 (MQ=255)                                                                                caGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCt                                                <  2:187688/140‑1 (MQ=255)                                                                                 aGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTc                                               >  2:379552/1‑140 (MQ=255)                                                                                  gCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCa                                              <  1:253204/140‑1 (MQ=255)                                                                                  gCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCa                                              >  2:671593/1‑140 (MQ=255)                                                                                      tCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCt                                                <  1:972816/134‑1 (MQ=255)                                                                                      tCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCt                                                >  2:972816/1‑134 (MQ=255)                                                                                             gTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACAt                                   >  2:574980/1‑140 (MQ=255)                                                                                                aaCAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGc                                >  1:856298/1‑140 (MQ=255)                                                                                                 aCAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGca                               <  2:17504/140‑1 (MQ=255)                                                                                                  cAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAAcc                                           <  1:712910/127‑1 (MQ=255)                                                                                                  cAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCGGCTGTTTTCCCTCAAcc                                           >  2:712910/1‑127 (MQ=255)                                                                                                     cAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGcaccac                           <  2:325860/140‑1 (MQ=255)                                                                                                     cAACGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGcaccac                           >  2:98652/1‑140 (MQ=255)                                                                                                      aaCGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACg                          <  1:98652/140‑1 (MQ=255)                                                                                                      aaCGAGTGGCGCTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACg                          <  2:169431/140‑1 (MQ=255)                                                                                                                 cTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGt               <  2:856298/140‑1 (MQ=255)                                                                                                                 cTGGCACGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGt               <  1:574980/140‑1 (MQ=255)                                                                                                                       cGAGCCTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCa         <  1:671593/140‑1 (MQ=255)                                                                                                                           ccTTTAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGAc     <  1:154533/140‑1 (MQ=255)                                                                                                                              ttAATGGTTGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTg  <  1:696520/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    TAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTG  >  NC_000913/520089‑520353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑